Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting Polygonatum sibiricum polysaccharides

A technology for polysaccharide polysaccharide and polygonum polysaccharides, which is applied in the field of extracting polysaccharide polysaccharides, can solve the problems of low yield and low extraction efficiency.

Inactive Publication Date: 2019-12-13
湖南天泉生物科技有限公司
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the extraction efficiency of above-mentioned extraction separation method is not high, and yield is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A kind of ultrasonic wave assists the technology of extracting polygonatum polysaccharide, comprises the steps:

[0016] Step 1: Screening, cleaning, slicing and grinding the Rhizoma Polygonatum into powder to obtain Rhizoma Polygonatum powder, the water content of which is 2.1%;

[0017] Step 2: Add Rhizoma Polygonatum Powder to a round bottom flask, add petroleum ether with a material-to-liquid ratio of 1:20, heat and reflux for extraction for 0.5 h, filter through gauze to obtain a filter residue, and reflux the filter residue with 20 times the volume of 95% ethanol at 70°C Extracted for 1.5h, air-dried to obtain the sample.

[0018] Step 3: Weigh the sample and add distilled water. Use an ultrasonic extractor to extract by ultrasonic oscillation for 50 minutes at room temperature. The ultrasonic power is 120W. After completion, centrifuge with a centrifuge at a speed of 4000r / min for 20 minutes. , extracting the supernatant, mixing the lower precipitate with 3 time...

Embodiment 2

[0022] A kind of ultrasonic wave assists the technology of extracting polygonatum polysaccharide, comprises the steps:

[0023] Step 1: Screening, cleaning, slicing and grinding the Rhizoma Polygonatum into powder to obtain Rhizoma Polygonatum powder, the water content of which is 1.8%;

[0024] Step 2: Add Rhizoma Polygonatum powder to a round bottom flask, add petroleum ether at a material-to-liquid ratio of 1:25, heat and reflux for extraction for 1 hour, filter through gauze to obtain a filter residue, and extract the filter residue with 20 times the volume of 95% ethanol at 70°C for 1 hour , air-dried to obtain samples.

[0025] Step 3: Weigh the sample and add distilled water. Use an ultrasonic extractor to extract by ultrasonic oscillation for 50 minutes at room temperature. The ultrasonic power is 150W. After completion, use a centrifuge to centrifuge at a speed of 4000r / min for 25 minutes. , extracting the supernatant, mixing the lower precipitate with 3 times the vo...

Embodiment 3

[0029] A kind of ultrasonic wave assists the technology of extracting polygonatum polysaccharide, comprises the steps:

[0030] Step 1: Screening, cleaning, slicing and grinding the Rhizoma Polygonatum into powder to obtain Rhizoma Polygonatum powder, the water content of which is 1.8%;

[0031] Step 2: Add Rhizoma Polygonatum powder to a round-bottomed flask, add petroleum ether, heat and reflux for extraction for 1 hour, filter through gauze to obtain a filter residue, extract the filter residue with 20 times the volume of 95% ethanol at 70°C for 2 hours, and air-dry to obtain a sample.

[0032] Step 3: Weigh the sample and add distilled water. Use an ultrasonic extractor to perform ultrasonic vibration extraction at room temperature for 60 minutes. The ultrasonic power is 180W. , extracting the supernatant, mixing the lower precipitate with 3 times the volume of 70% ethanol, filtering, mixing the filtrate and the supernatant, and concentrating by distillation under reduced ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a process for ultrasonic wave-assisted extraction of Polygonatum sibiricum polysaccharides. The process comprises: 1, screening Polygonatum filipes, cleaning, slicing, and grinding into powder; 2, adding the Polygonatum filipes powder into a round-bottom flask, adding petroleum ether, carrying out heating reflux extraction for 0.5-1 h, filtering, carrying out reflux extraction on the filter residue for 1-2 h with 95% ethanol at a temperature of 70 DEG C, and carrying out air drying to obtain a sample; 3, weighing the sample, adding distilled water, carrying out ultrasonic oscillation extraction for 30-70 min by using an ultrasonic wave extractor at a room temperature, carrying out standing layering after ultrasonic treatment, extracting the supernatant, mixing the lower-layer precipitate and 70% ethanol accounting for 3 times the volume of the lower-layer precipitate, filtering, mixing the filtrate and the supernatant, and carrying out pressure reducing distillation concentration to prepare a concentrated solution; 4, extracting the concentrated solution with chloroform-n-butyl alcohol 2-3 times, performing liquid separation, adding active carbon into the upper-layer liquid, carrying out decolorization, and performing suction filtration; and 5, centrifugally filtering the filtrate after the suction filtration to obtain a filter cake, and washing the filter cake respectively with 95% ethanol, absolute alcohol and diethyl ether to obtain the Polygonatum sibiricum polysaccharide refined product.

Description

technical field [0001] The invention belongs to the technical field of Polygonatum polysaccharide extraction, in particular to a method for extracting Polygonatum polysaccharide. Background technique [0002] Polygonatum Polygonatum is the dried rhizome of Polygonatum Polygonatum, Polygonatum Polygonatum or Polygonatum Polyflora, which is a traditional Chinese medicine in my country and belongs to Chinese herbal medicine with the same origin of medicine and food. This taste is sweet and flat, and has the effects of nourishing the middle and Qi, invigorating the spleen and moistening the lungs, nourishing the kidneys and strengthening the bones. It mainly contains polygonatum polysaccharides, saponins, niacin, quinones, amino acids and trace elements, etc. It has various pharmacological effects such as delaying aging, enhancing immune function, regulating blood lipids, lowering blood sugar, and anti-tumor. Among them, polysaccharide is the main active ingredient, which has s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 杨理政
Owner 湖南天泉生物科技有限公司