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Method for jointly producing polyhydroxyalkanoate by residual sludge and Baijiu wastewater

A technology of polyhydroxyalkanoate and excess sludge, applied in the fields of environmental protection and resource utilization, can solve the problems of low VFAs yield, limit the yield of PHA synthesis from activated sludge, etc., achieve high utilization value, and promote output increase. , the effect of promoting synthesis

Pending Publication Date: 2019-12-13
HOHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] A large number of literatures show that liquor wastewater and excess sludge have a synergistic effect on acid production by anaerobic fermentation, and the yield of VFAs produced by anaerobic fermentation using excess sludge or liquor wastewater alone is low, which limits the subsequent synthesis of activated sludge Yield of PHA

Method used

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  • Method for jointly producing polyhydroxyalkanoate by residual sludge and Baijiu wastewater
  • Method for jointly producing polyhydroxyalkanoate by residual sludge and Baijiu wastewater

Examples

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Effect test

Embodiment 1

[0025] The method for joint production of polyhydroxyalkanoate by utilizing excess sludge and liquor waste water comprises the following steps:

[0026] (1) Fill 6.1mL of liquor wastewater and 415mL of residual sludge added with crushed sludge into a sealed brine bottle with an effective volume of 450mL. Adjust the pH to 8. In order to ensure that the reaction is carried out under anaerobic conditions, use nitrogen to purify for about 3 minutes and then cork the bottle tightly. Finally, put the brine bottle in a constant temperature shaker with a temperature of 35 ° C and a speed of 180 rpm for the reaction. The maximum total accumulation of VFAs was 5438mgCOD / L, corresponding to a reaction time of 4 days.

[0027] (2) A sequencing batch reactor with an effective volume of 9L is used to control the SBR reaction platform with a timing controller, so that it can perform "feeding-aeration-sedimentation-drainage-feeding" according to the Feast-Famine mode cycle. The SBR was perf...

Embodiment 2

[0030] The method for joint production of polyhydroxyalkanoate by utilizing excess sludge and liquor waste water comprises the following steps:

[0031] (1) 10.2 mL of liquor wastewater and 346 mL of residual sludge added with crushed sludge were placed in a sealed brine bottle with an effective volume of 450 mL. Adjust the pH to 8. In order to ensure that the reaction is carried out under anaerobic conditions, use nitrogen to purify for about 3 minutes and then cork the bottle tightly. Finally, put the brine bottle in a constant temperature shaker with a temperature of 35 ° C and a speed of 180 rpm for the reaction. The maximum total accumulation of VFAs was 7563mgCOD / L, corresponding to a reaction time of 4 days.

[0032] (2) A sequencing batch reactor with an effective volume of 9L is used to control the SBR reaction platform with a timing controller, so that it can perform "feeding-aeration-sedimentation-drainage-feeding" according to the Feast-Famine mode cycle. The SBR...

Embodiment 3

[0035] The method for joint production of polyhydroxyalkanoate by utilizing excess sludge and liquor waste water comprises the following steps:

[0036] (1) 15.3 mL of liquor wastewater and 260 mL of residual sludge added with crushed sludge were placed in a sealed brine bottle with an effective volume of 450 mL. Adjust the pH to 8. In order to ensure that the reaction is carried out under anaerobic conditions, use nitrogen to purify for about 3 minutes and then cork the bottle tightly. Finally, put the brine bottle in a constant temperature shaker with a temperature of 35 ° C and a speed of 180 rpm for the reaction. The maximum total accumulation of VFAs was 9057mgCOD / L, corresponding to a reaction time of 10 days.

[0037] (2) A sequencing batch reactor with an effective volume of 9L is used to control the SBR reaction platform with a timing controller, so that it can perform "feeding-aeration-sedimentation-drainage-feeding" according to the Feast-Famine mode cycle. The SBR ...

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Abstract

The invention discloses a method for jointly producing polyhydroxyalkanoate by residual sludge and Baijiu wastewater, wherein the method comprises the steps: performing combined anaerobic fermentationculture on the Baijiu wastewater and the residual sludge added with a part of crushed sludge under a mixing condition of a certain proportion, and taking a fermentation product as a substrate for synthesizing polyhydroxyalkanoate; domesticating activated sludge by a sequencing batch reactor, carrying out cyclic operation of feeding, aeration, static settling, drainage and feeding according to a Feast-Famine mode, namely, intermittently controlling the duration of filling and lack of substrates in the reactor; and adding a fermentation product and the domesticated activated sludge into the reactor according to a certain proportion, and synthesizing polyhydroxyalkanoate by taking oxygen as an electron acceptor under the conditions that the pH value is equal to 8 and the synthesis temperature is 25 + / -1 DEG C. According to the method, the resource utilization of the Baijiu wastewater and the reduction of the residual sludge can be realized, the bioplastic polyhydroxyalkanoate with high utilization value can be produced, and the production of the polyhydroxyalkanoate from low-cost raw materials is realized.

Description

technical field [0001] The invention relates to the technical field of environmental protection and resource utilization, in particular to a method for efficiently producing polyhydroxyalkanoate by using the combined fermentation product of excess sludge and liquor wastewater as an electron donor. Background technique [0002] PHA (polyhydroxyalkanoate) is a natural polymer biomaterial. It not only has the physical and chemical properties of synthetic plastics, but also has other unique and excellent properties such as biocompatibility, gas barrier, biodegradability, and optical activity. Therefore, PHA is widely used in the fields of packaging, medical treatment, agriculture, fishery and cosmetics, and is valued by the scientific and industrial circles. At present, industrial synthesis of PHA mainly adopts bacterial fermentation method and genetic engineering method, but its disadvantages such as high operating cost and complicated process seriously restrict the marketizati...

Claims

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Application Information

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IPC IPC(8): C12P7/62C02F11/04C02F3/28
CPCC12P7/625C02F11/04C02F3/28Y02W10/10
Inventor 翁佳玉方芳董锦云李雨笑陈玲珑张晶思杨洁
Owner HOHAI UNIV
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