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Injectable natural triterpene compound antibacterial hydrogel and preparation method thereof

A triterpene compound and injection-type technology, which is applied in the direction of antibacterial drugs, local antibacterial agents, drug combinations, etc., can solve the problems of increased preparation cost and low application value, and achieve good antibacterial effect and high mechanical strength

Active Publication Date: 2019-12-20
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method often requires cumbersome gel molecule synthesis and purification processes, which increases the cost of preparation and leads to low practical application value.

Method used

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  • Injectable natural triterpene compound antibacterial hydrogel and preparation method thereof
  • Injectable natural triterpene compound antibacterial hydrogel and preparation method thereof
  • Injectable natural triterpene compound antibacterial hydrogel and preparation method thereof

Examples

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Embodiment 1

[0031] After in-depth research, the inventor has prepared a triterpenoid hydrogel with intrinsic antibacterial properties and injectable properties. The present invention makes full use of the antibacterial property and amphiphilic molecular structure of natural triterpene compounds, and selects the hydrogel with injectability and antibacterial property through gelation experiments and hydrogel antibacterial, cell activity and hemolysis experiments. and preparation method.

[0032] In this example, a mixture of glycyrrhizic acid and glycyrrhetinic acid in pentacyclic triterpenoids was used as a gel molecule to prepare a hydrogel, and PBS buffer with pH=7 was used as a solvent. Wherein, the molar fraction of glycyrrhizic acid is 70%, and the molar fraction of glycyrrhetinic acid is 30%, and the specific preparation method is as follows:

[0033] First, a suspension solution of glycyrrhizic acid and glycyrrhetinic acid with a concentration of 3.5 mM was prepared in a container,...

Embodiment 2

[0035] In this example, a mixture of glycyrrhizic acid and ursolic acid in pentacyclic triterpenoids was used as gel molecules to prepare a hydrogel, and PBS buffer with pH=7.4 was used as a solvent. Wherein, the molar fraction of glycyrrhizic acid is 60%, and the molar fraction of ursolic acid is 40%, and the specific preparation method is as follows:

[0036] First, prepare a suspension solution of glycyrrhizic acid and ursolic acid at a concentration of 5 mM in a container, wherein the molar fraction of glycyrrhizic acid is 60%, and the molar fraction of ursolic acid is 40%; The solution was ultrasonically treated for 2 min to promote the uniform dispersion of glycyrrhizic acid and ursolic acid molecules in the aqueous solution; then the suspended solution was placed in an oven at 97 °C and heated for 150 min, at which time the solution changed from a turbid state to a transparent state; finally, the transparent The solution was placed in an environment of 25° C. until the ...

Embodiment 3

[0038] In this example, a mixture of glycyrrhizic acid and ginsenosides in tetracyclic triterpenoids was used as gel molecules to prepare hydrogel, and PBS buffer with pH=7.2 was used as solvent. Wherein, the molar fraction of glycyrrhizic acid is 90%, and the molar fraction of ginsenoside is 10%, and the specific preparation method is as follows:

[0039]First, prepare a mixed suspension solution with a concentration of 2mM glycyrrhizic acid and ginsenoside in a container with PBS buffer solution of pH=7.2, wherein the molar fraction of glycyrrhizic acid is 90%, and the molar fraction of ginsenoside is 10%; The suspension was ultrasonically treated for 15 min in an environment to promote the uniform dispersion of the two gel molecules in the aqueous solution; then the suspension was placed in an oven at 90°C and heated for 120 min, at which time the solution changed from a turbid state to a transparent state; finally The transparent solution was placed in an environment of 25...

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Abstract

The invention relates to the field of biological antibacterial hydrogel materials, in particular to injectable natural triterpene compound antibacterial hydrogel and a preparation method thereof. Thehydrogel disclosed by the invention is obtained by employing a mixture of glycyrrhizic acid and a pentacyclic triterpene compound or a tetracyclic triterpene compound as a gel molecule and phosphoricacid buffer salt solution (PBS) as a gel solvent, and utilizing non-covalent bond acting force formed between mixture through a micromolecule self-assembly mechanism. The prepared hydrogel can show antibacterial property without adding antibacterial drugs, and the mechanical property of the hydrogel is remarkably improved. The preparation method of the injectable antibacterial hydrogel is simple,a preparation process of the injectable antibacterial hydrogel can be simplified, and the application field of the triterpenoids is expanded. Therefore, a novel injectable antibacterial material withbiomedical application characteristics and mechanical strength can be prepared.

Description

Technical field: [0001] The invention belongs to the field of biological antibacterial hydrogel materials, in particular to an injectable natural triterpenoid antibacterial hydrogel. Background technique: [0002] Low-molecular-weight hydrogels (referred to as hydrogels) are generally obtained by small molecular compounds in water interacting through non-covalent bonds to form a relatively stable three-dimensional network structure. Among them, injectable hydrogels are widely used in tissue engineering, drug delivery, wound healing and other fields. However, since the application scenarios of hydrogels are mostly in humid environments, if they do not have antibacterial properties, they will be easily infected by bacteria, which will eventually lead to serious infection of the target site. Therefore, the preparation of an injectable antibacterial hydrogel has become a research hotspot in the field of biomaterials. [0003] In view of the special properties and application s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K31/704A61K31/56A61K31/575A61P31/04A61P31/02A61P17/02A61L26/00
CPCA61K9/0019A61K9/06A61K31/56A61K31/575A61K31/704A61L26/0061A61L26/0066A61L26/008A61L2300/404A61L2300/412A61L2400/06A61P17/02A61P31/02A61P31/04A61K2300/00
Inventor 胡君张浩吴剑桥于霞
Owner BEIJING UNIV OF CHEM TECH
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