Application of multilineage-differentiating stress-enduring cell, drug for treating diabetes and preparation method of drug

A diabetes drug and a technology for diabetes, which are applied to the drug for the treatment of diabetes and the preparation thereof. The application field of multi-lineage differentiation and continuous stress cells in the preparation of the drug for the treatment of diabetes achieves strong self-renewal ability, protection of pancreatic islet cells, and great clinical application value. Effect

Pending Publication Date: 2019-12-20
NANTONG UNIVERSITY
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, there is no drug that can treat diabetes thoroughly, long-term, and without side effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of multilineage-differentiating stress-enduring cell, drug for treating diabetes and preparation method of drug
  • Application of multilineage-differentiating stress-enduring cell, drug for treating diabetes and preparation method of drug
  • Application of multilineage-differentiating stress-enduring cell, drug for treating diabetes and preparation method of drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of multilineage differentiated persistent stress cells

[0027] Professional doctors performed bone marrow aspiration on the donor's anterior superior iliac spine, and extracted 1ml of bone marrow. Mix the bone marrow and phosphate buffered saline solution at a ratio of 1:1 and add it to the upper layer of the lymphocyte separation medium. After centrifugation, the liquid is divided into four layers. The uppermost layer accounts for about 1 / 2 of the liquid column and is a light red plasma layer; Accounting for 1 / 4 of the liquid column, it is a clear lymphocyte separation liquid layer; at the junction of the two layers is a milky white cloud-like layer with a height of about 5 mm, which is the mononuclear cell layer (this layer is the cell layer to be extracted); The bottom layer is tightly attached to the wall of the test tube and is dark red, mainly composed of denser red blood cell layer. Take the second layer of cells, use DMEM / F12 complete medium contai...

Embodiment 2

[0030] Multilineage differentiated continuous stress cells for the treatment of diabetic model mice

[0031] 1) Streptozotocin-induced mouse diabetes model

[0032] Streptozotocin (STZ) injection modeling: STZ selectively destroys certain species of pancreatic islet β cells, which can cause diabetes in many animals. Because of its low tissue toxicity and high animal survival rate, it is an ideal animal model that is widely used at home and abroad to study diabetes and its chronic complications. The preparation of animal models of type 1 diabetes and type 2 diabetes is related to the dose of STZ injection: when a large dose of STZ is injected, it will directly cause extensive damage to the islet β cells, resulting in a type 1 diabetes model; while injecting a small amount of STZ, due to It only destroys the function of a part of the islet β cells, making the peripheral tissue insensitive to insulin. At the same time, it is fed with high-calorie feed. The combination of the two...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides application of a multilineage-differentiating stress-enduring cell in preparation of a drug for treating diabetes and further provides the drug for treating the diabetes. The drug is characterized by at least comprising a cell suspension of the multilineage-differentiating stress-enduring cell. The drug for treating the diabetes can also contain pharmaceutically acceptable auxiliary materials. Besides, the invention further provides a preparation method of the drug for treating the diabetes. The preparation method is characterized by comprising the following steps: (1) preparing and culturing the multilineage-differentiating stress-enduring cell; and (2) preparing the cell suspension of the multilineage-differentiating stress-enduring cell. The application of the multilineage-differentiating stress-enduring cell in preparation of the drug for treating the diabetes provides a new direction for treatment of the diabetes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the application of multilineage differentiated persistent stress cells in the preparation of drugs for treating diabetes, the drug for treating diabetes and a preparation method thereof. Background technique [0002] Diabetes is caused by genetic factors, immune dysfunction, microbial infection and its toxins, free radical toxins, mental factors and other pathogenic factors acting on the body to cause hypofunction of pancreatic islets and insulin resistance. Clinically, hyperglycemia is the main feature. Typical cases may show polyuria, polydipsia, polyphagia, weight loss, etc., that is, "three more and one less" symptoms. Once diabetes (blood sugar) Poor control will lead to complications, leading to failure of the kidneys, eyes, feet and other parts of the disease, and can not be cured. Diabetes is divided into type 1 diabetes, type 2 diabetes, gestational diabe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/10A61K35/51A61P25/00A61P29/00C12N5/0775
CPCA61K35/28A61K9/10A61K35/51A61P29/00A61P25/00C12N5/0663
Inventor 陈罡王晓冬赵雅玉戴玉娟
Owner NANTONG UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap