Detection primer, detection reagent kit and detection method for multiple viruses of parrots, and application

Abstract

The invention belongs to the technical field of detection of animal pathogeny molecules, and particularly relates to a detection primer, a detection reagent kit and a detection method for multiple viruses of parrots, and an application. According to the reagent kit disclosed by the invention, a CP gene for psittacine beak and feather disease viruses, a VP1 gene for budgerigar fledgling disease viruses, and a hexon gene for avian adenovirus are used as formworks, a specific primer is used, through PCR, specific nucleic acid probes are marked through digoxigenin, through dot blot, the probes canbe used for detecting the existence of psittacine beak and feather disease viruses, the budgerigar fledgling disease viruses or avian adenovirus nucleic acid in pathologic material samples. Through the utilization of the reagent kit, DNA in pathologic material tissue samples is extracted to be subjected to dot blot, detection can be completed in 24h-36h, the result is reported, the specificity ishigh, and the accuracy is high. If nucleic acid extracts of the same sample are respectively dropped on three membranes, besides, corresponding nucleic acid probes are added, and three different viruses can be detected at the same time.

Description

technical field [0001] The invention belongs to the technical field of molecular detection of animal pathogens, and in particular relates to a parrot multiple virus detection primer, a detection kit, a virus detection method and an application. Background technique [0002] Parrots belong to the class of Aves, Psittaciformes, and Psittacidae in biological classification, and are pets that people like to keep. All kinds of parrots are kept as pets for their beautiful feathers and good at learning human language. They are artificially bred in all countries in the world and are very important economic animals. In the past few decades, research on diseases in parrots has been scarce compared to large-scale poultry. In fact, caged ornamental birds are very susceptible to various diseases, especially viral infectious diseases, and once infected, the mortality rate is often high. [0003] Common viral infectious diseases in parrots include psittacine beak and featherdisease (PBFD...

AI Technical Summary

Problems solved by technology

The clinical symptoms of several viral diseases that often occur in parrots are very similar. It is difficult to make an accurate diagnosis only through apparent symptoms and gross autopsy. It must be confirmed by pathological observation and PCR identification. However, the existing detection The method has certain limitations and non-specificity

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