Standard substance for calibration, and preparation method and application thereof

Abstract

The invention provides a standard substance for calibration, and a preparation method and application thereof. The standard substance for calibration is polystyrene particle dispersion liquid; and thepolystyrene particle dispersion liquid is used as the standard substance, wherein the particle diameter of polystyrene particles in the polystyrene particle dispersion liquid is similar to the average diameter of bacteria, bacterium particles can be well simulated, and the polystyrene particle dispersion liquid has high uniformity, high dispersibility, high stability and sedimentation and agglomeration difficulty. Compared with a conventionally used standard substance using barium sulfate particles for simulating bacterium turbidity, the standard substance for calibration has the advantages that the stability is higher; the long-time storage and use are more convenient; a preparation method of the polystyrene particle dispersion liquid is simple; the raw materials can be easily obtained;the price is low; the realization is easy; and the application is convenient. Through the calibration on a McFarland turbidity meter by the polystyrene particle dispersion liquid, the polystyrene particle dispersion liquid can well simulate the bacterium turbidity, so that the McFarland turbidity meter calibrated through the polystyrene particle dispersion liquid has high test accuracy.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and relates to a standard substance for calibration and a preparation method and application thereof. Background technique [0002] The McFarland turbidity meter is mainly used to measure the McFarland turbidity of bacteria. By measuring the degree of light scattering produced by bacteria, the content of bacteria can be quantitatively characterized. It can be widely used in the pharmaceutical industry, epidemic prevention industry, and hospitals. High levels will have a greater adverse effect on human health, and severe cases can be fatal. Therefore, it is necessary to detect the content of bacteria, especially the accurate detection of bacteria content is more important, so it is very important to calibrate the Maxwell turbidimeter. It is necessary, but there is still no certified standard material for bacterial turbidity. Although there are commercial bacterial standard turbidimetr...

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Problems solved by technology

[0002] The McFarland turbidity meter is mainly used to measure the bacterial McFarland turbidity. By measuring the degree of light scattering produced by the bacteria, the content of the bacteria can be quantitatively characterized, which can be widely used It is used in the pharmaceutical industry, epidemic prevention industry and hospitals, etc. Excessive bacterial content will have a greater adverse effect on human health, and severe cases can be fatal. Therefore, it is very necessary to detect the bacterial content, especially those that can accurately detect bacteria The content is more important, so it is very necessary to calibrate the Maxwell turbidimeter, but there is still no certified standard material for bacterial turbidity. Although there are commercial bacterial standard turbidimetric tubes on the market, they have not passed metrological investigation , the quality is uneven, the quantity value lacks traceability, and the stability and uniformity cannot be guaranteed

[0003]The traditional bacterial standard turbidimetric tube uses barium sulfate precipitation as the bacterial simulation particle. The advantage of this material is that it has a mature and classic preparation method. Blocks are required to be fully shaken before each use, which is inconvenient to use and the uniformity cannot be guaranteed, thus greatly affecting the accuracy of the test

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