Preparation method of dopamine-modified molybdenum oxide quantum dots, and application thereof in trace uranium detection
A molybdenum oxide, quantum dot technology, applied in measurement devices, fluorescence/phosphorescence, instruments, etc., can solve the problems of cumbersome preprocessing steps, complicated and expensive equipment, and achieve the effect of ensuring accuracy
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Embodiment 1
[0034] Preparation and characterization of MoOxQDs-DA:
[0035] (1) Add 10mg molybdenum oxide powder to 10mL10wt%H 2 o 2 Neutralize and disperse evenly, place in a water bath at 37°C and stir for 1 hour, then add NaOH to adjust the pH to 5-6, then centrifuge at 8000r / min for 10min to obtain the supernatant, which is MoO X QD S ;
[0036] (2) Take 10mL of MoO prepared in step (1) X QD S Add 1.25 mL of 8 mg / mL dopamine hydrochloric acid solution, mix well, and react in the dark for 2 hours at room temperature, then dialyze with a dialysis bag with a molecular weight cut-off of 1 kD for 24 hours, and replace deionized water every 6 hours during the period. After the dialysis is completed, the retentate is MoO X QD S -DA.
[0037] For the prepared MoO X QD S -DA for testing, the results are attached figure 1 shown, from the attached figure 1 It can be seen from the absorption spectrum that after dialysis treatment, MoOxQDs alone has no characteristic absorption peak in ...
Embodiment 2
[0039] Detection of the interaction of uranium(VI) with MoOxQDs and MoOxQDs-DA:
[0040] First prepare 132.0 μM uranyl nitrate solution: accurately measure 1.0 mL of 13.2 mM uranium standard solution in a 100 mL volumetric flask, add a certain amount of distilled water and adjust the pH to 4.5 with nitric acid, and finally make up to volume to obtain a 132.0 μM uranyl nitrate solution.
[0041] Interaction of uranium(Ⅵ) with MoOxQDs and MoOxQDs-DA:
[0042] In order to illustrate the positive role of dopamine modification in the detection of uranium (Ⅵ), the fluorescence spectra of the interaction between uranium (Ⅵ) and MoOxQDs and the MoOxQDs-DA prepared in Example 1 were measured respectively. The results are shown in the attached figure 2 shown, from the attached figure 2It can be seen that the fluorescence intensity of MoOxQDs and MoOxQDs-DA decreased after interacting with uranium(Ⅵ), but the decrease rate of MoOxQDs-DA was significantly lower than that of MoOxQDs, in...
Embodiment 3
[0044] Determination of optimal pH:
[0045] Because uranium (Ⅵ) and MoOxQDs-DA may exhibit different forms under different pH conditions, this example experiments the influence of pH on the interaction between the two, as shown in Table 1, in the range of pH 4.0 to 6.0, The degree of fluorescence quenching of MoOxQDs-DA by uranyl ions gradually increased, and then decreased with the increase of pH, and reached the optimal standard at pH6.0.
[0046] Fluorescence intensity of the mixed solution at different pHs in table 1
[0047] pH MoOxQDs-DA MoOxQDs-DA+uranium(Ⅵ) 4.0 2128.29 1685.81 5.0 2264.50 1484.40 6.0 2286.30 1002.66 7.0 2286.83 1031.08 8.0 2301.18 1167.98
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