A kind of Bacillus cereus ma23, bacteria agent, preparation method and application
A technology of Bacillus cereus and microbial agent, applied in the field of agricultural microorganisms, can solve the problems of pathogenic bacteria resistance to drugs, easy pollution of the environment, human and animal poisoning, etc., and achieve the effects of reducing the loss of agricultural products, being environmentally friendly and low in cost
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[0039] The preparation method of above-mentioned microbial inoculum, the concrete steps are as follows:
[0040] Step 1. Strain activation:
[0041] The cryopreserved Bacillus cereus MA23 was inoculated on a nutrient agar plate medium for activation and culture, and cultured at 28-32°C for 20-24 hours; then a single colony was picked and inoculated on a nutrient agar slant medium for expansion culture , cultured at 28-32 °C for 20-24 h; finally, the bacteria were eluted with 10 mL of sterile water, and the obtained eluate was used as the inoculum for seed culture;
[0042] Step 2. Preparation of seed solution:
[0043] Inoculate the inoculum obtained in step 1 into the seed medium, the volume ratio of the seed medium and the inoculum used is 1:0.5% to 1:5%, at 28 to 32° C. and the initial pH value is 5.5 to 7.5. Shake culture for 14-20h to obtain seed liquid;
[0044] Wherein, the components of the seed medium used and their mass percentages are: glucose 1.0%-2.0%, peptone ...
Embodiment 1
[0077] The preparation method of MA23 microbial inoculum, the specific steps are as follows:
[0078] Step 1. Bacterial activation: Inoculate the cryopreserved Bacillus cereus MA23 on a nutrient agar plate medium for activation and culture, and culture at 30°C for 20 hours; then pick a single colony and inoculate it on a nutrient agar slant medium for expansion. The cells were further cultured at 30°C for 20 hours; the bacteria were eluted with sterile water, and the obtained eluate was used as the inoculum for seed culture.
[0079] In this example, Bacillus cereus strain MA23 was stored in an environment of -80°C.
[0080] The pH value of the nutrient agar plate medium and the nutrient agar slant medium is 7.0 to 7.4, and the components and their mass percentages are: peptone 1.0%, beef extract 0.5%, NaCl 0.5%, agar 2.0%, and the balance is Water, the preparation method can be prepared with reference to the national standard GB / T 4789.28-2003.
[0081] Step 2. Preparation ...
Embodiment 2
[0087] The preparation method of microbial inoculum, the concrete steps are as follows:
[0088] Step 1. Strain activation:
[0089] The cryopreserved Bacillus cereus strain MA23 was inoculated on a nutrient agar plate medium for activation and culture, and cultured at 30 °C for 24 hours; then a single colony was picked and inoculated on a nutrient agar slant medium for expansion culture, and the culture was carried out at 30 °C. Cultivated for 20h; then eluted the bacteria with sterile water, and the obtained eluate was used as the inoculum for seed culture.
[0090] In this example, Bacillus cereus strain MA23 was stored in an environment of -80°C.
[0091] The pH value of the nutrient agar plate medium and the nutrient agar slant medium is 7.0 to 7.4, and the components and their mass percentages are: peptone 1.0%, beef extract 0.5%, NaCl 0.5%, agar 2.0%, and the balance is water , the preparation method can be prepared with reference to the national standard GB / T 4789.28...
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