MicroRNA328 regulating tert gene expression and its applications

Abstract

The invention discloses microRNA328 regulating TERT gene expression and its application. The present invention provides the use of microRNA328 or a substance that inhibits its expression. The present invention finds that miR328 has a significant inhibitory effect on the expression level of TERT mRNA in Hela cells, and an oligonucleotide inhibitor of miR-328 has a significant up-regulation effect on the expression level of TERT gene mRNA in Hela cells. The inhibitor of miR-328 significantly increased the angiogenic capacity of EPCs. EPCs transfected with the inhibitor of miR-328 significantly inhibited the differentiation of NSCs into glial cells. Studies have shown that using miRNA as a target or tool to enhance the activity of telomerase provides a theoretical basis for the treatment of neurological diseases such as vascular dementia (VD), and has potential clinical application value.

Description

technical field

[0001] The invention belongs to the field of biotechnology, in particular to microRNA328 regulating TERT gene expression and its application. Background technique

[0002] Vascular dementia (VD) is a clinical syndrome caused by brain dysfunction caused by cerebrovascular disease, resulting in brain intelligence and cognitive dysfunction, mainly manifested as learning, memory, thinking and other obstacles. It is the second leading cause of dementia after Alzheimer's disease (AD). With the acceleration of the aging process of the world population, the number of patients with VD has increased significantly. According to epidemiological statistics, the prevalence of elderly people over 65 years old is 1%-4%, and the prevalence of elderly people aged 85 years is as high as 14%- 16%. Alzheimer's disease including VD has become the fourth leading cause of death among the elderly after tumor, heart disease and stroke. Therefore, it is an urgent task for medical wo...

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