A strain of bacillus polymyxa and its application

A technology of Bacillus polymyxa and strains, applied in the field of Bacillus polymyxa strains, can solve the problems of unsatisfactory control effects of chemical pesticides and environmental pollution

Active Publication Date: 2021-05-11
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the serious occurrence of fungal diseases of P. polymyxa and the unsatisfactory control effects of chemical pesticides and the problems of environmental pollution, a Paenibacillus polymyxa strain and its application

Method used

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  • A strain of bacillus polymyxa and its application
  • A strain of bacillus polymyxa and its application
  • A strain of bacillus polymyxa and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The Bacillus polymyxa strain of the present invention, the Bacillus polymyxa strain is PBSZ123, the preservation name is Paenibacillus polymyxa, and it is preserved in the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), and the address of the preservation unit It is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. Date of deposit: August 14, 2019; Collection number: CGMCC No.18386.

[0027] The gene sequence of the Bacillus polymyxa strain is the 16S nucleotide sequence shown in SEQ ID No.1.

[0028] The bacillus polymyxa bacterial agent prepared by the bacillus polymyxa bacterial strain of the present invention. Its active ingredient is at least one of the following (a) (b) (c):

[0029] (a) the fermentation culture of the bacillus polymyxa bacterial strain described in claim 1;

[0030] (b) ultrasonic lysing supernatant of the bacillus polymyxa bacterial strain cell described in clai...

Embodiment 2

[0038] Strain acquisition:

[0039] Bacillus polymyxa PBSZ123, preservation number CGMCC No.18386, was collected from Yunnan Province to collect healthy Pygmus polymyxa tubers, soaked in 75% ethanol for 1 min, rinsed with sterile water for 3 times, and blotted the surface moisture with sterile filter paper under conventional aseptic operation; % HgCl2 for 3 min, rinsed with sterile water for 10 times, and blotted the surface moisture with sterile filter paper. Use a sterile scalpel to peel off the epidermis of the surface-sterilized Pachyphylla tuber, cut into cubes with a size of 0.5 cm, place them in NA medium plates, and culture them at 28°C for 2 days. After bacteria grow on the plate, the plate was streaked and purified 3-5 times to obtain Bacillus polymyxa PBSZ123.

Embodiment 3

[0041] Strain identification:

[0042] 1. Observation of Morphological Characteristics

[0043] Morphological characteristics of the colony: The colony of the strain PBSZ123 is light yellow on the NA plate, with a smooth surface, raised, neat edges, and a round shape. Morphological characteristics of bacteria: Gram-positive bacteria with spores, rod-shaped bacteria, size (0.32-0.64) μm × (0.84-1.30) μm, such as figure 2 shown.

[0044] 2.16S sequence analysis

[0045] After the genomic DNA of the strain was extracted by liquid nitrogen grinding, the primer: 27F

[0046] 5'-AGAGTTTGATCCTGGCTCAG3'; 1492R

[0047] 5'-TACGGCTACCTTGTTACGACTT-3' was PCR amplified.

[0048] After gel electrophoresis, it was sent to Bioengineering (Shanghai) Co., Ltd. for sequencing. The full length of the sequence is 1404bp (see SEQ). The resulting sequence was submitted to the GenBank database for BLAST analysis and comparison, and it was found that the strain with high homology to PBSZ123 be...

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Abstract

The invention discloses a bacillus polymyxa strain and application thereof. The Bacillus polymyxa strain of the present invention, the Bacillus polymyxa strain is PBSZ123, the preserved name is Bacillus polymyxa (Paenibacillus polymyxa), and it is preserved in the General Microorganism Center (CGMCC) of China Microorganism Culture Preservation Management Committee, the depository unit The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Date of deposit: August 14, 2019; Collection number: CGMCC No.18386. The bacillus polymyxa of the present invention is isolated from the endophyte of the polyphylla, and has a good inhibitory effect on the main pathogenic bacteria of the polyphylla, Botrytis cinerea, Phoma leaf spot, and Polychaete spp.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a strain of bacillus polymyxa and its application. Background technique [0002] In recent years, with the sharp increase in the demand for traditional Chinese medicine resources, the wild resources of traditional Chinese medicine have been severely damaged, and the transformation of wild resources into cultivation has become the only way to meet market demand. Diseases rarely occur in wild Chinese medicinal materials, and even if they do, they will not cause disasters. After changing from wild to artificial cultivation, their growth environment changes. Management measures such as seed transfer have created the conditions for the occurrence of diseases, and diseases are easy to spread within the population, between fields, and between regions, resulting in aggravated diseases of cultivated medicinal materials. [0003] Parispolyphyllavar.yunnanensis (Franch.) Hand is a pl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/06A01P3/00C12R1/01
CPCC12N1/06C12N1/20C12N1/205C12R2001/01
Inventor 张仲凯郑宽瑜陈永对张洁郑雪赵立华吴阔苏晓霞
Owner 云南省农业科学院生物技术与种质资源研究所
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