Unlock instant, AI-driven research and patent intelligence for your innovation.

Bacillus subtilis producing gamma-poly(glutamic acid) and application thereof

A Bacillus subtilis, polyglutamic acid technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as low yield, and achieve a simple process, suitable for industrialization promotion, good market prospects and economic benefits. Effect

Inactive Publication Date: 2019-12-31
NANJING SHINEKING BIOTECH CO LTD
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a kind of Bacillus subtilis producing γ-polyglutamic acid, so as to solve the problems such as low yield in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Utilize the fermentation of Bacillus subtilis XK producing γ-polyglutamic acid to produce γ-polyglutamic acid, the specific steps are as follows:

[0032] (1) Preparation of seed liquid: inoculate Bacillus subtilis into a mixture containing sodium glutamate (45g / L), citric acid (50g / L), bean cake powder (40g / L), potassium dihydrogen phosphate (1g / L) In the fermentation medium with water, the shaker speed is 120-200rpm, and cultured at 28-36°C for 10-18h to OD 660 Greater than 5.0;

[0033] (2) Fermentation culture: the seed liquid in the step (1) is inoculated with sodium glutamate (45g / L), citric acid (50g / L), bean cake powder (40g / L), In the fermentation medium of potassium dihydrogen phosphate (1g / L), manganese sulfate (0.05g / L), and magnesium sulfate (1.2g / L), the liquid volume of the fermentation medium in a 7.5L fermenter is 4L, and the aeration ratio is controlled at 1.0vvm, the initial pH is 6.5, cultured at 28°C for 48h;

[0034] (3) The fermentation broth i...

Embodiment 2

[0036] The preparation of the seed solution was the same as in Example 1.

[0037](1) Fermentation culture: the seed liquid is inoculated in the fermentation medium with 4% inoculation amount, contains sodium glutamate (65g / L), citric acid (34g / L), bean cake powder (20g / L) in the medium ), potassium dihydrogen phosphate (1g / L), manganese sulfate (0.09g / L), magnesium sulfate (1.8g / L) in the fermentation medium, the liquid volume of the 7.5L fermentation tank fermentation medium is 4L, and the aeration ratio Control at 1.5vvm, initial pH is 6.9, culture at 32°C for 52h;

[0038] (2) The fermentation broth is diluted (concentration is below 1g / L), and then the molecular weight and yield of γ-polyglutamic acid in the fermentation broth are detected by high performance liquid chromatography. The molecular weight of the produced γ-polyglutamic acid was about 89kDa, and the yield of γ-polyglutamic acid was 42.7g / L.

Embodiment 3

[0040] The preparation of the seed solution was the same as in Example 1.

[0041] (1) Fermentation culture: the seed liquid is inoculated in the fermentation medium with the inoculum of 3%, contains sodium glutamate (51g / L), citric acid (29g / L), bean cake powder (37g / L) in the medium ), potassium dihydrogen phosphate (1g / L), manganese sulfate (0.02g / L), magnesium sulfate (1.1g / L) in the fermentation medium, the liquid volume of the 7.5L fermentation tank fermentation medium is 4L, and the aeration ratio Controlled at 1.2vvm, the initial pH is 6.5, cultured at 34°C for 36h;

[0042] (2) The fermentation broth is diluted (concentration is below 1g / L), and then the molecular weight and yield of γ-polyglutamic acid in the fermentation broth are detected by high performance liquid chromatography. The molecular weight of the produced γ-polyglutamic acid is about 38kDa, and the yield of γ-polyglutamic acid is 36.8g / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses bacillus subtilis producing gamma-poly(glutamic acid). The bacillus subtilis producing the gamma-poly(glutamic acid) is preserved in China Center for Type Culture Collection under the classification name Bacillus Subtilis with the bacterial strain number XK and the preservation number CCTCC M 2016605 from November 1, 2016 on. The invention further discloses application of the bacillus subtilis XK in production of gamma-poly(glutamic acid). Compared with the prior art, gamma-poly(glutamic acid) with three different molecular weight ranges (namely lower than 100kda, 100-700kda and higher than 700kda) can be obtained by taking the bacillus subtilis XK as a starting bacterial strain as well as adjusting concentrates of magnesium sulphate and magnesium sulfate in fermentation media; and moreover, yields of the gamma-poly(glutamic acid) with the three different molecular weight ranges are all up to 35 g / L or above. The bacillus subtilis producing gamma-poly(glutamic acid) can be well applied in the fields of fertilizers, cosmetics, sewage processing and the like. In addition, preparation technology of the bacillus subtilis producing gamma-poly(glutamic acid) is simple and suitable for industrial promotion; and thus, good market prospects and economic benefits are ensured.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a gamma-polyglutamic acid-producing bacillus subtilis and an application thereof. Background technique [0002] γ-polyglutamic acid is an anionic polymer connected by D-glutamic acid monomers and L-glutamic acid monomers through amide bonds formed by α-amino groups and γ-carboxyl groups. Because of the water solubility, biodegradability, edibility and non-toxicity of γ-polyglutamic acid to humans and the environment, γ-polyglutamic acid and its derivatives have been widely used in various fields in recent years , can be used as a flocculant in water treatment, an ingredient in cosmetics and medicines, and as a fertilizer additive, etc. [0003] At present, the commercial production of γ-polyglutamic acid is mainly obtained through microbial submerged liquid fermentation. The commonly used production strain Bacillus subtilis uses monosodium glutamate as the main substrate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/02C12R1/125
CPCC12N1/20C12P13/02
Inventor 徐虹冯小海许宗奇梁金丰詹伊婧
Owner NANJING SHINEKING BIOTECH CO LTD