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Method for extracting collagen from cowhide

A collagen and extraction method technology, applied in the field of collagen extraction from cowhide, can solve problems such as long extraction time and increase protein impurities, and achieve the effects of improving thermal denaturation temperature, preventing excessive hydrolysis and reducing the probability of thermal denaturation

Active Publication Date: 2019-12-31
JINAN PANSHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is mainly to reduce the loss rate of collagen by using complex enzymes to extract at a lower temperature. Although it can reduce the denaturation of collagen to a certain extent, the extraction time is too long, and it is easy to cause excessive hydrolysis of the dissolved collagen. while increasing the impurities in the protein

Method used

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  • Method for extracting collagen from cowhide

Examples

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Effect test

Embodiment 1

[0026] A method for extracting collagen in cowhide, comprising the following steps:

[0027] (1) Cowhide pretreatment: put the cowhide into compound enzyme solution A whose temperature is 22°C which is 2 times its mass, and soak it at a constant temperature for 150 minutes, drain it, rinse it with clean water, and cut the cowhide into small pieces of 3-5cm ;Put the cowhide block into the protective solution and soak for 50min, drain and dry in the air;

[0028] (2) Miscellaneous protein removal: Put the cowhide piece treated in step (1) into a compound alkali solution at 30°C for 15 minutes, then soak it in a slightly acidic solution for 5 minutes, drain it, and rinse it with clean water Afterwards, dry and set aside;

[0029] (3) Preparation of kraft pulp: mix the processed cowhide block, ice water and protective agent in step (2), and send it into a homogenizer for homogenization for 10 minutes to obtain kraft pulp;

[0030] (4) Collagen extraction: add its mass 0.005% com...

Embodiment 2

[0041] A method for extracting collagen in cowhide, comprising the following steps:

[0042] (1) Cowhide pretreatment: put the cowhide into compound enzyme solution A which is 2.4 times its mass and the temperature is 24°C, soak it at a constant temperature for 120 minutes, drain it, rinse it with clean water, and cut the cowhide into small pieces of 3-5cm ;Put the cowhide block into the protective solution and soak for 55min, drain and dry in the air;

[0043] (2) Miscellaneous protein removal: Put the cowhide piece treated in step (1) into a compound alkali solution at 32°C for 12 minutes, then soak it in a slightly acidic solution for 5 minutes, drain it, and rinse it with clean water Afterwards, dry and set aside;

[0044] (3) Preparation of kraft pulp: mix the processed cowhide block, ice water and protective agent in step (2), and send it into a homogenizer for homogenization for 8 minutes to obtain kraft pulp;

[0045] (4) Collagen extraction: add its mass 0.005% comp...

Embodiment 3

[0056] A method for extracting collagen in cowhide, comprising the following steps:

[0057] (1) Cowhide pretreatment: put the cowhide into the compound enzyme solution A whose temperature is 25°C which is 3 times its mass, and soak it at a constant temperature for 120 minutes, drain it, rinse it with clean water, and cut the cowhide into small pieces of 3-5cm ;Put the cowhide block into the protective solution and soak for 60min, drain and dry in the air;

[0058] (2) Miscellaneous protein removal: Put the cowhide piece treated in step (1) into a compound alkali solution at 35°C for 10 minutes, then soak it in a slightly acidic solution for 5-6 minutes, drain it, and wash it with clean water After rinsing, dry and set aside;

[0059] (3) Preparation of kraft pulp: mix the processed cowhide block, ice water, and protective agent in step (2), and send it into a high-speed homogenizer for homogenization for 10 minutes to obtain kraft pulp;

[0060] (4) Collagen extraction: add...

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Abstract

The invention relates to the technical field of collagen extraction and particularly relates to a method for extracting collagen from cowhide. The method comprises the following steps: (1) pretreatingcowhide, namely putting cowhide into a complex enzyme solution A, performing constant-temperature soaking treatment, draining and flushing the soaked cowhide, and cutting the cowhide into pieces; andputting the cowhide pieces into a protection solution, performing soaking treatment, draining the cowhide pieces and drying the cowhide pieces in the air; (2) removing impure protein, namely puttingthe cowhide pieces into a complex alkali solution, performing treatment, soaking the cowhide pieces in a subacid solution, draining and flushing the cowhide pieces, and drying the cowhide pieces in the air for later use; (3) preparing cowhide pulp, namely mixing the cowhide pieces, ice water and a protective agent, feeding the mixture into a high-speed homogenizer, and performing homogenizing to prepare the cowhide pulp; (4) extracting collagen, namely adding complex B into the cowhide pulp, performing extraction under a stirring status, and performing inactivation; and separating solid and liquid to obtain an extracted liquid for later use; and (5) separating the collagen. The extraction method has relatively high purity and extraction rate of collagen and excellent bioactivity; and the process time is relatively short.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a method for extracting collagen from cowhide. Background technique [0002] my country is a big meat production and consumption country in the world. Beef accounts for a large proportion of meat consumption, while by-products such as cowhide are mainly used in the leather industry, resulting in a low utilization rate. Cowhide is rich in collagen, and collagen has high application value in the field of food and medical first-class cosmetics. [0003] Collagen has a molecular weight of about 300kDa and is composed of three peptide chains with similar molecular weights. The three peptide chains are intertwined and form a stable triple helix structure through hydrogen bonds. Collagen is rich in glycine (Gly), proline and hydroxyproline, forming a typical (Gly-X-Y) structure (X, Y represent other amino acids), and there are (Gly-X-Y) on each peptide chain ) repeat struc...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCC07K14/78C12P21/06
Inventor 谭春萍程忠黄广君黄福标黄春兰
Owner JINAN PANSHENG BIOTECH
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