A kind of extraction method of collagen in cowhide
A collagen and extraction method technology, applied in the field of collagen extraction from cowhide, can solve the problems of long extraction time and increased protein impurities, achieve good biological activity, improve thermal denaturation temperature, and shorten the extraction process time
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Embodiment 1
[0026] A method for extracting collagen in cowhide, comprising the following steps:
[0027] (1) Cowhide pretreatment: Put the cowhide in twice its mass at a temperature of 22 o Soak in the compound enzyme solution A of C for 150 minutes at a constant temperature, drain, rinse with clean water, cut the cowhide into small pieces of 3-5 cm; put the cowhide into the protection solution for 50 minutes, drain out, and dry in the air;
[0028] (2) Miscellaneous protein removal: put the cowhide block treated in step (1) into 30 o After being treated in the compound alkali solution of C for 15 minutes, put it into the slightly acidic solution for soaking treatment for 5 minutes, drain it, rinse it with clean water, dry it, and set it aside;
[0029] (3) Preparation of kraft pulp: mix the processed cowhide block, ice water, and protective agent in step (2), and send it to a homogenizer for homogenization for 10 minutes to prepare kraft pulp;
[0030] (4) Collagen Extraction: Add 0.00...
Embodiment 2
[0041] A method for extracting collagen in cowhide, comprising the following steps:
[0042] (1) Cowhide pretreatment: Put the cowhide in a temperature 2.4 times its mass at 24 o Soak in the compound enzyme solution A of C for 120 minutes at constant temperature, drain, rinse with clean water, cut the cowhide into small pieces of 3-5 cm; put the cowhide into the protection solution for 55 minutes, drain out, and dry in the air;
[0043] (2) Miscellaneous protein removal: put the cowhide block processed in step (1) into 32 o After being treated in the compound alkali solution of C for 12 minutes, put it into the slightly acidic solution for soaking treatment for 5 minutes, drain it, rinse it with clean water, dry it, and set it aside;
[0044] (3) Preparation of kraft pulp: mix the processed cowhide block in step (2), ice water, and protective agent, and send it to a homogenizer for homogenization for 8 minutes to prepare kraft pulp;
[0045] (4) Collagen extraction: Add 0.00...
Embodiment 3
[0056] A method for extracting collagen in cowhide, comprising the following steps:
[0057] (1) Cowhide pretreatment: put the cowhide into 3 times its mass at a temperature of 25 o Soak the compound enzyme solution A of C at a constant temperature for 120 minutes, drain, rinse with clean water, cut the cowhide into small pieces of 3-5 cm; put the cowhide pieces into the protection solution and soak for 60 minutes, drain out, and dry in the air;
[0058] (2) Miscellaneous protein removal: put the cowhide block processed in step (1) into 35 o After being treated in the compound alkali solution of C for 10 minutes, put it into the slightly acidic solution for soaking treatment for 5-6 minutes, drain it, rinse it with clean water, dry it, and set aside;
[0059] (3) Preparation of kraft pulp: mix the processed cowhide in step (2), ice water, and protective agent, and send it to a high-speed homogenizer for homogenization for 10 minutes to prepare kraft pulp;
[0060] (4) Collag...
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