A kind of extraction method of collagen in cowhide

A collagen and extraction method technology, applied in the field of collagen extraction from cowhide, can solve the problems of long extraction time and increased protein impurities, achieve good biological activity, improve thermal denaturation temperature, and shorten the extraction process time

Active Publication Date: 2021-07-02
JINAN PANSHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is mainly to reduce the loss rate of collagen by using complex enzymes to extract at a lower temperature. Although it can reduce the denaturation of collagen to a certain extent, the extraction time is too long, and it is easy to cause excessive hydrolysis of the dissolved collagen. while increasing the impurities in the protein

Method used

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  • A kind of extraction method of collagen in cowhide

Examples

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Effect test

Embodiment 1

[0026] A method for extracting collagen in cowhide, comprising the following steps:

[0027] (1) Cowhide pretreatment: Put the cowhide in twice its mass at a temperature of 22 o Soak in the compound enzyme solution A of C for 150 minutes at a constant temperature, drain, rinse with clean water, cut the cowhide into small pieces of 3-5 cm; put the cowhide into the protection solution for 50 minutes, drain out, and dry in the air;

[0028] (2) Miscellaneous protein removal: put the cowhide block treated in step (1) into 30 o After being treated in the compound alkali solution of C for 15 minutes, put it into the slightly acidic solution for soaking treatment for 5 minutes, drain it, rinse it with clean water, dry it, and set it aside;

[0029] (3) Preparation of kraft pulp: mix the processed cowhide block, ice water, and protective agent in step (2), and send it to a homogenizer for homogenization for 10 minutes to prepare kraft pulp;

[0030] (4) Collagen Extraction: Add 0.00...

Embodiment 2

[0041] A method for extracting collagen in cowhide, comprising the following steps:

[0042] (1) Cowhide pretreatment: Put the cowhide in a temperature 2.4 times its mass at 24 o Soak in the compound enzyme solution A of C for 120 minutes at constant temperature, drain, rinse with clean water, cut the cowhide into small pieces of 3-5 cm; put the cowhide into the protection solution for 55 minutes, drain out, and dry in the air;

[0043] (2) Miscellaneous protein removal: put the cowhide block processed in step (1) into 32 o After being treated in the compound alkali solution of C for 12 minutes, put it into the slightly acidic solution for soaking treatment for 5 minutes, drain it, rinse it with clean water, dry it, and set it aside;

[0044] (3) Preparation of kraft pulp: mix the processed cowhide block in step (2), ice water, and protective agent, and send it to a homogenizer for homogenization for 8 minutes to prepare kraft pulp;

[0045] (4) Collagen extraction: Add 0.00...

Embodiment 3

[0056] A method for extracting collagen in cowhide, comprising the following steps:

[0057] (1) Cowhide pretreatment: put the cowhide into 3 times its mass at a temperature of 25 o Soak the compound enzyme solution A of C at a constant temperature for 120 minutes, drain, rinse with clean water, cut the cowhide into small pieces of 3-5 cm; put the cowhide pieces into the protection solution and soak for 60 minutes, drain out, and dry in the air;

[0058] (2) Miscellaneous protein removal: put the cowhide block processed in step (1) into 35 o After being treated in the compound alkali solution of C for 10 minutes, put it into the slightly acidic solution for soaking treatment for 5-6 minutes, drain it, rinse it with clean water, dry it, and set aside;

[0059] (3) Preparation of kraft pulp: mix the processed cowhide in step (2), ice water, and protective agent, and send it to a high-speed homogenizer for homogenization for 10 minutes to prepare kraft pulp;

[0060] (4) Collag...

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Abstract

The invention relates to the technical field of collagen extraction, in particular to a method for extracting collagen from cowhide, comprising the following steps: (1) cowhide pretreatment: placing cowhide in compound enzyme solution A for constant temperature soaking treatment, leaching, rinsing, Cut into small pieces; put the cowhide block into the protective solution for soaking treatment, drain it out, and dry it in the air; (2) Removal of impurity protein: put the cowhide block into the compound alkali solution for processing, and then put it into the slightly acid solution Soak treatment, drain out, rinse with air, dry for use; (3) Kraft pulp preparation: mix the cowhide block, ice water, and protective agent, and send it into a high-speed homogenizer to homogenize to obtain Kraft pulp; (4) Collagen extraction: adding compound enzyme B to the obtained kraft pulp, stirring and extracting, and inactivating; solid-liquid separation, to obtain an extract for use; (5) collagen separation. The extraction method provided by the invention has high purity and extraction rate of collagen, and has good biological activity; and the process time is short.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a method for extracting collagen from cowhide. Background technique [0002] my country is a big meat production and consumption country in the world. Beef accounts for a large proportion of meat consumption, while by-products such as cowhide are mainly used in the leather industry, resulting in a low utilization rate. Cowhide is rich in collagen, and collagen has high application value in the field of food and medical first-class cosmetics. [0003] Collagen has a molecular weight of about 300kDa and is composed of three peptide chains with similar molecular weights. The three peptide chains are intertwined and form a stable triple helix structure through hydrogen bonds. Collagen is rich in glycine (Gly), proline and hydroxyproline, forming a typical (Gly-X-Y) structure (X, Y represent other amino acids), on each peptide chain (Gly-X-Y ) repeat structure appears, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14
CPCC07K14/78C12P21/06
Inventor 谭春萍程忠黄广君黄福标黄春兰
Owner JINAN PANSHENG BIOTECH
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