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Preparation method and application of a kind of artificial exosome complex

A compound and exosome technology, applied in the field of biochemistry, can solve the problems of hyaluronic acid with large molecular weight, limited skin care durability, and ineffective deep skin cell absorption, etc., and achieve the effect of controllable operation steps and promotion of absorption

Active Publication Date: 2021-06-04
上海澄穆实业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hyaluronic acid, also known as hyaluronic acid, is a natural moisturizing lubricant. Due to the large molecular weight of hyaluronic acid, it cannot be effectively absorbed by deep skin cells, and the durability of skin care is limited.

Method used

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  • Preparation method and application of a kind of artificial exosome complex
  • Preparation method and application of a kind of artificial exosome complex
  • Preparation method and application of a kind of artificial exosome complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Detection of skin irritation by artificial exosome complex

[0037] Detection method:

[0038] Recruit 10 volunteers, use the newly prepared artificial exosomes with a concentration of 1mg / mL to evenly smear on the inner side of the left wrist, and apply ddH2O on the inner side of the right wrist as a control group, and let it stand for 20 minutes after application, and continue to observe the changes on the inner side of the wrist , and recorded whether the volunteers had irritating sensations such as pain and itching.

[0039] Experimental results such as figure 1 shown.

[0040] Table 1. Statistical results of artificial exosomes on skin test

[0041]

[0042] Experimental results:

[0043] The 10 volunteers did not observe redness and swelling after skin irritation within 20 minutes after applying the artificial exosomes, and none of the 10 volunteers felt irritating pain or itching.

Embodiment 2

[0044] Example 2. Cytotoxicity detection of artificial exosomes

[0045] Detection method:

[0046] Human immortalized epidermal cells Hacat were cultured, cells were digested and plated in 96-well plates, 5000 cells / well. Set the gradient concentration of artificial exosomes to incubate Hacat cells, continue to culture for 48 hours, observe the morphology of the cells during this period, and finally use the MTT method to detect the effect on cell proliferation.

[0047] Experimental results such as figure 2 , 3 shown.

[0048] Experimental results:

[0049] Artificial exosomes did not show obvious cytotoxicity at the cellular level, and had no effect on cell proliferation.

Embodiment 3

[0050] Example 3. Detection of the delivery efficiency of active substances by artificial exosomes

[0051] Detection method:

[0052] Using artificial exosomes as carriers, the activity of green fluorescent plasmid pCDNA3.1-eGFP delivered into cells was detected on Hacat cells.

[0053] Experimental results such as Figure 4 shown.

[0054] Experimental results:

[0055] Artificial exosomes have a good effect of coating active substances, and can effectively deliver active substances into cells.

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Abstract

The invention belongs to the technical field of biochemistry, and in particular relates to a preparation method and application of an artificial exosome complex. The preparation raw materials of the complex are linear polyethyleneimine (PEI) and hyaluronic acid (HA). The HA-PEI preparation process of the present invention can obtain linear polyethyleneimine, but most of the polyethyleneimines are branched at present; the HA-PEI preparation scheme can quantitatively prepare HA-PEI with a specific molecular weight; The steps are controllable and large-scale industrial production is possible; HA‑PEI can promote the absorption of the encapsulated active substance by deep skin cells through the ligand activity of hyaluronic acid and the membrane permeability of linear polyethyleneimine.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a preparation method and application of an artificial exosome complex. Background technique [0002] The effectiveness of skin care products mainly lies in the absorption efficiency of active substances. Currently, there are few effective active substance delivery media on the market. Because linear polyacetylimide has both hydrophilic groups and lipophilic groups, it can form a bimolecular layer through the form of hydrophilic groups inside and outside lipophilic groups, and wrap substances to form microcapsules. Microcapsules It can use its own membrane fusion to fuse with the cell membrane and deliver inclusions into the cell. However, since the microcapsules utilize membrane fusion properties, they do not have cell selectivity. Hyaluronic acid, also known as hyaluronic acid, is a natural moisturizing lubricant. Due to the large molecular weight of hyaluroni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/84A61K8/73A61Q19/00
CPCA61K8/84A61K8/735A61Q19/00A61K2800/592A61K8/14A61K2800/57A61K9/0014A61K9/5192A61K9/5107A61K9/1275A61K47/36A61K47/34A61K31/785A61K31/728C08G73/0206A61K2300/00
Inventor 李俊翔朱才彬
Owner 上海澄穆实业发展有限公司
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