Application of NADH and salt thereof to preparation of skin pigment inhibitor
A skin pigment and inhibitor technology, applied in the application field of NADH in the preparation of skin pigment inhibitors, can solve problems such as the research on the influence of NADH skin pigment, to prevent or treat skin diseases, increase skin pigmentation, or prevent skin pigmentation Effects of DNA damage
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Embodiment 1
[0025] In vitro experiments on the effect of NADH on the production of melanin in cells:
[0026] Adjust the concentration of human melanoma cell A375 (purchased from Shenzhen University) to 1×10 5 cells / mL, seeded in a 6-well plate, 2 mL per well. After adherence, the cells were grouped with 25-200 μmol·L -1 NADH treatment (sample group), control group with 200μmol L -1 Natural human type I collagen was treated, and the blank group was treated with normal saline. After 48 hours, it was washed 3 times with pre-cooled PBS and scraped off to collect them in sterile EP tubes. The method of Tsuboi (1998) was used for the determination of melanin content: centrifuge at 1500rpm for 5min, discard the supernatant, lyse the cells with 0.5mL of 1mol L-1NaOH solution containing 10% DMSO, ultrasonically break for 30min, and bathe in water at 90°C for 2h to make the cell clumps completely After lysis, centrifuge at 3000rpm for 15min, take the supernatant to a 96-well plate, measure the ...
Embodiment 2
[0032] Human trials of NADH in inhibiting skin melanin and reducing skin damage:
[0033] Taking 18 healthy women aged 30-40 as objects, they were randomly divided into 6 groups with 3 people in each group. Experimental group 4: smear 25-200 μmol·L on the measurement site within one hour before the measurement -1 NADH in range, once a day. Control group 1: Apply 200 μmol L in the same way -1 of kojic acid. Blank group 1: only smear normal saline. An opaque adhesive plaster with a diameter of 1.5 cm was attached to the smeared part of the subject's upper arm, and then 1.5 to 2 times the minimum erythema dose (MED; Minimum erythma dose) of each subject was irradiated with ultraviolet B to induce darkening of the skin. The changes of melanin index and erythema index over time after UV irradiation were measured with a spectrophotometer CM-1000.
[0034] According to the method of Mao Peikun et al. "Cosmetic Functional Evaluation and Analysis Method" (China Light Industry Pres...
Embodiment 3
[0045] A pharmaceutical composition that uses NADH as an active component to inhibit skin pigmentation is disclosed:
[0046] Mix 5 parts of Atractylodes macrocephala extract, 1 part of Poria cocos extract, 3 parts of Cornus officinalis extract, 4 parts of Polyporus terrestris extract, 1 part of Shayuan Tribulus extract and 1 part of licorice extract, add 8 times the amount of water in terms of mass After dissolving and stirring evenly, add 5 parts of NADH and 5 parts of NMN, fully stir and mix at room temperature, then raise the temperature to 50°C and concentrate in vacuo to obtain the composition. The composition is made into tablets according to conventional methods and pharmaceutical auxiliary materials.
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