Metarhizium anisopliae MANGS71814 and application of metarhizium anisopliae MANGS71814 in prevention and treatment of phthorimaea operculella
A technology of MANGS71814 and Metarhizium anisopliae, which is applied in the field of microorganisms, can solve problems such as human and animal safety hazards, pesticide residues, ecological environment impacts, etc., and achieve the effects of high spore germination rate, difficult production, and large spore production
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0019] Example 1: Isolation and identification of Metarhizium anisopliae MANGS71814
[0020] 1.1 Material
[0021] 1.1.1 Test strains
[0022] The strain was collected on August 8, 2019 from the pupae of diseased lepidopteran pests in Nangong Mountain, Mengyuan Town, Xishuangbanna Autonomous Prefecture, Yunnan Province.
[0023] 1.1.2 Isolation and cultivation of strains
[0024] The collected third-instar larvae of the diseased potato tuber moth were placed in a 5mL sterile EP tube and brought back to the laboratory. Using the streaking method, they were placed in SDAY medium (40g glucose, 10g peptone, and 10g yeast extract. Agar 20g, pH natural), cultured in a light incubator at (25±1)°C, L / / D=16h / / 8h, humidity 80%. After 7 days of cultivation, the conidia were transferred to a new medium for purification. The purified strain was stored in a refrigerator at 4°C using SDAY slant medium.
[0025] 1.1.3 Observation of colony morphology
[0026] The isolated and purified fungi were conne...
Example Embodiment
[0030] Example 2: Determination of the pathogenicity of Metarhizium anisopliae MANGS71814 to potato tuber moth eggs, larvae and pupae
[0031] 1 material
[0032] 1.1.1 Tested insects
[0033] Potato tuber moths were collected from potato fields in Xuanwei City, Yunnan Province, and kept indoors for 5 consecutive generations. The test was carried out under the conditions of temperature 25±1℃, relative humidity (RH) 60±5%, and photoperiod L:D=16:8 Under the intelligent artificial incubator (RG-300). Potato tuber moths are reared with fresh potato pieces and leaves.
[0034] 1.1 Preparation of spore suspension
[0035] Inoculate the isolated strain on SDAY medium, and after it produces a large number of conidia (about 20 days), scrape the conidia on the surface of the medium with a sterile scalpel and place it in 0.02% sterile Tween-80 , Made into 2×10 3 –2×10 8 Pcs / mL spore suspension.
[0036] 1.1.2 Dipping method
[0037] Select healthy third-instar larvae of the potato tuber moth wit...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap