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Metarhizium anisopliae MANGS71814 and application of metarhizium anisopliae MANGS71814 in prevention and treatment of phthorimaea operculella

A technology of MANGS71814 and Metarhizium anisopliae, which is applied in the field of microorganisms, can solve problems such as human and animal safety hazards, pesticide residues, ecological environment impacts, etc., and achieve the effects of high spore germination rate, difficult production, and large spore production

Active Publication Date: 2020-01-10
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, chemical control methods are mainly used for the control of potato tuber moth at present, and the insect has developed strong resistance to many pesticides
In addition, problems such as pesticide residues caused by the use of chemical control methods have hidden dangers to human and animal safety and risks to the ecological environment.

Method used

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  • Metarhizium anisopliae MANGS71814 and application of metarhizium anisopliae MANGS71814 in prevention and treatment of phthorimaea operculella
  • Metarhizium anisopliae MANGS71814 and application of metarhizium anisopliae MANGS71814 in prevention and treatment of phthorimaea operculella
  • Metarhizium anisopliae MANGS71814 and application of metarhizium anisopliae MANGS71814 in prevention and treatment of phthorimaea operculella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Isolation and identification of Metarhizium anisopliae MANGS71814

[0020] 1.1 Materials

[0021] 1.1.1 Tested strains

[0022] The strain was collected from pupae of diseased Lepidoptera pests in Nangong Mountain, Mengyuan Town, Xishuangbanna Autonomous Prefecture, Yunnan Province on August 8, 2019.

[0023] 1.1.2 Isolation and cultivation of strains

[0024] The collected 3rd instar larvae of the diseased potato tuber moth were placed in a 5mL sterile EP tube and brought back to the laboratory. Using the streaking method, in the SDAY medium (glucose 40g, peptone 10g, yeast extract powder 10g, Agar 20g, pH natural), cultivated in a light incubator at (25±1)°C, L / / D=16h / / 8h, humidity 80%. After 7 days of culture, the conidia were transferred to a new medium for purification. Purified strains were stored in a 4°C refrigerator using SDAY slant medium.

[0025] 1.1.3 Colony morphology observation

[0026] Inoculate the isolated and purified fungi into SDAY...

Embodiment 2

[0030] Example 2. Determination of pathogenicity of Metarhizium anisopliae MANGS71814 to potato tuber moth eggs, larvae and pupae

[0031] 1 material

[0032] 1.1.1 Tested insects

[0033] Potato tuber moths were collected in the potato field of Xuanwei City, Yunnan Province, and were continuously raised indoors for 5 generations. The experiment was carried out under the conditions of temperature 25±1°C, relative humidity (RH) 60±5%, and photoperiod L:D=16:8. carried out in the intelligent artificial incubator (RG-300). Potato tuber moths are reared on fresh potato cubes and leaves.

[0034] 1.1 Preparation of spore suspension

[0035] Inoculate the isolated bacterial strains on the SDAY medium, and after it produces a large number of conidia (about 20 days), scrape the conidia on the surface of the medium with a sterile scalpel and place them in 0.02% sterile Tween-80 , formulated as 2 x 10 3 – 2×10 8 individual / mL spore suspension.

[0036] 1.1.2 Dipping method

[00...

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Abstract

The invention provides metarhizium anisopliae MANGS71814 and an application of the metarhizium anisopliae MANGS71814 in prevention and treatment of phthorimaea operculella. The metarhizium anisopliaeMANGS71814 is preserved in China General Microbiological Culture Collection Center on August 21, 2019, the preservation number is CGMCC No.18121, and the preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing. The obtaining process of the metarhizium anisopliae MANGS71814 comprises the following steps: performing separation on collected three-year-old larvae of the phthorimaea operculella suffering from diseases to obtain wild metarhizium anisopliae strains, performing culture on an SDAY culture medium, transferring conidia to a new culture medium, performing purification, and performing single spore separation to obtain the purified metarhizium anisopliae MANGS71814. The metarhizium anisopliae MANGS71814 provided by the invention is a biocontrol fungus capableof being used for preventing and treating the phthorimaea operculella, has the characteristics of strong pathogenicity to the phthorimaea operculella, environmental protection, no pollution and difficulty in generating drug resistance, and can be widely used for preventing and treating eggs, larvae and pupae of the phthorimaea operculella.

Description

technical field [0001] The application belongs to the technical field of microorganisms, and in particular relates to a Metarhizium anisopliae MANGS71814 and its application in the control of potato tuber moth. Background technique [0002] Potato tuber moth [Phthorimaea operculella (Zeller)] belongs to the order Lepidoptera, and belongs to the family of Lepidoptera. It is a plant pest of Solanaceae mainly distributed in the tropics and subtropics, and it is especially harmful to potatoes. In recent years, with global climate change, the distribution range of the insect has gradually spread, and the damage to tomato and tobacco, which are economic crops of Solanaceae, has also increased year by year. [0003] In the prior art, chemical control methods are mainly used for the prevention and control of potato tuber moth at present, and the insect has developed strong resistance to many pesticides. In addition, problems such as pesticide residues caused by the use of chemical ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02A01P7/04C12R1/645
CPCC12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 肖关丽陈斌郑亚强杜广祖和淑琪桂富荣李正跃
Owner YUNNAN AGRICULTURAL UNIVERSITY
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