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Specific binding peptide of polycaprolactone nanoparticles as well as screening method and application thereof

A technology of polycaprolactone and nanoparticles, applied in peptides, material inspection products, instruments, etc., can solve the problems of poor biocompatibility and high toxicity of fluorescent nanophotopoles

Active Publication Date: 2020-01-14
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

[0004] The purpose of the present invention is to provide a specific binding peptide of polycaprolactone nanoparticles, its screening method and application, so as to solve the problems of high toxicity, potential carcinogenic risk and poor biocompatibility in the prior art. question

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  • Specific binding peptide of polycaprolactone nanoparticles as well as screening method and application thereof
  • Specific binding peptide of polycaprolactone nanoparticles as well as screening method and application thereof
  • Specific binding peptide of polycaprolactone nanoparticles as well as screening method and application thereof

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with specific embodiments. It should be understood that the following examples are only used to illustrate the present invention but not to limit the scope of the present invention.

[0031] 1. Experimental reagents

[0032] Polycaprolactone (PCL), surfactant (Pluronic F127) and tetrahydrofuran (THF) were purchased from Sigma-Aldrich (Germany).

[0033] The phage dodecapeptide kit used in the phage screening process was purchased from NEB Co., Ltd. (USA), the M13 phage single-stranded genomic DNA rapid extraction kit was purchased from Haoxin Biotechnology Co., Ltd. (Hangzhou), bovine serum albumin (BSA), Isopropyl-β-D-thiogalactopyranoside (IPTG), 5-bromo-4-chloro-3-indole-β-D-galactopyranoside (X-gal), and tetracycline were purchased from Yi Saint Biotechnology Co., Ltd. (Shanghai), sodium citrate, trishydroxymethylaminomethane (Tris), polyethylene glycol 8000, sodium chloride, potassium chloride, ...

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Abstract

The invention provides a specific binding peptide of polycaprolactone nanoparticles as well as a screening method and an application thereof, and the screening method comprises the following steps: 1)preparing a polycaprolactone coating in a pore plate; 2) screening out two specific binding peptides by using the pore plate with the polycaprolactone coating prepared in the step 1) by adopting a phage display technology; and 3) verifying the two specific binding peptides obtained in the step 2) by adopting the polycaprolactone nanoparticles. According to the invention, two specific binding peptides of the polycaprolactone nanoparticles are obtained through screening; the polypeptide sequences of the specific binding peptide are DGSMLNRMRGFS and YALGRPSLQGPN respectively, the specific binding peptide can be coupled with biomolecules such as polypeptide and protein, and a new thought is expected to be provided for construction of an intelligent nano sensor in a complex environment.

Description

technical field [0001] The invention relates to the field of fluorescent nano-optics, and more specifically relates to a specific binding peptide of polycaprolactone nanoparticles, a screening method and application thereof. Background technique [0002] Fluorescent nano-optodes are a type of optical sensor developed based on electrochemical sensing theory. Previously, the concentration detection of a series of ions such as potassium, sodium, and calcium has been realized, and it has been successfully used in environmental monitoring and other fields. Traditional ion-selective nano-optodes usually use plasticizers (such as diisooctyl sebacate, DOS) and plasticized polyvinyl chloride (PVC) as membrane matrix materials, and ion exchangers, ionophores, and lipophilic pH The indicator (chromogenic ionophore) is used as the sensing component, and the ionophore is used to specifically identify the ion to be detected, and the concentration of the ion to be detected is reflected acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08G01N21/64G01N33/68
CPCC07K7/08G01N21/6428G01N33/68
Inventor 刘玥伶王平高营营燕蕊
Owner EAST CHINA UNIV OF SCI & TECH
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