Unlock instant, AI-driven research and patent intelligence for your innovation.

A strain of Trichoderma guizhou and its application

A technology of Trichoderma and mycelium, applied in fungi, fungicides, chemicals for biological control, etc., can solve problems such as excessive heavy metals, destruction of ecological balance, and decline in soil fertility

Active Publication Date: 2021-03-05
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Plant diseases caused by plant pathogenic bacteria pose a great threat to plant health, causing crop yield reduction or even failure
Although chemical control has always been the main measure to prevent and control plant diseases, the long-term use of chemical pesticides has caused serious decline in soil fertility, excessive heavy metals, and damage to ecological balance.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Trichoderma guizhou and its application
  • A strain of Trichoderma guizhou and its application
  • A strain of Trichoderma guizhou and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the isolation, purification and identification of Trichoderma strain

[0044] 1. Isolation and Purification of Trichoderma

[0045] Take soil samples from the forest area of ​​Milin County, Nyingchi Prefecture, Tibet, weigh 1g of soil samples, and dilute to 10 with sterile water -1 、10 -2 and 10 -3 Three concentration gradients, each concentration gradient takes 200 μl and spreads on PDA medium (potato 200g, glucose 20g, agar 20g, distilled water to 1000ml, sterilized at 121 ℃ and added to the medium at a final concentration of 300mg / l chloramphenicol) on the surface of a 9cm diameter petri dish. After inverting the plate and incubating at 25°C for 1–3 days, pick a single colony and transfer it to PDA slant medium.

[0046] 2. Species identification of Trichoderma strains

[0047] Biological features: see figure 1 , on CMD medium, after 3 days of culture at 25 ° C, the colony radius is 70–73 mm, and the plate is full in 3 days. The colonies are gr...

Embodiment 2

[0066] Embodiment 2, the application of Trichoderma

[0067] 1. Confrontation with plant pathogenic bacteria

[0068] Guizhou Trichoderma TC952 and Botrytis cinerea (Zhao Juan, Liu Ting, Liu Weicheng, et al. were selected from the preserved slant medium respectively. Screening and identification of Streptomyces against Botrytis cinerea [J]. Microbiology Bulletin, 2019,46 (10): 2548-2558), Fusarium graminearum (the fungus is stored in the China Agricultural Microorganism Culture Collection Center, the preservation number is: ACCC 31053) and Rhizoctonia solani (Rhizoctonia solani ACCC 36124) 3 plant pathogenic bacteria in Activate on the PDA medium, then use a 5mm diameter puncher to cut the bacterial block at the edge of the activated colony as the inoculum, and inoculate it on a 9cm petri dish containing PDA. The inoculum of TC952 and plant pathogenic bacteria are separated on the PDA plate 5 cm, and a plate inoculated with only plant pathogenic bacteria at one end was used a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Guizhou Trichoderma strain and its application. The Trichoderma strain of the present invention has strong habitat adaptability, fast growth rate and abundant conidia, and is effective against Botrytis cinerea, Fusarium graminearum and Rhizoctonia solani. It has obvious growth inhibitory effect, and the inhibitory rates of plate confrontation culture are 78.8%, 100% and 100% respectively. The strain can re-parasitize the above three plant pathogenic bacteria, and effectively kill the pathogenic bacteria by winding and invading the hyphae of the plant pathogenic bacteria. The bacterial strain controls cucumber blight caused by Rhizoctonia solani, and the control effect is 52.9%, and the control effect is high and stable. Guizhou Trichoderma TC952 is suitable for the effective control of crop diseases caused by the above pathogenic bacteria.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases, and in particular relates to a Trichoderma guizhou strain and application thereof. Background technique [0002] Plant diseases caused by phytopathogenic bacteria pose a great threat to plant health and cause crop yield reduction or even failure. Although chemical control has always been the main measure to prevent and control plant diseases, the long-term use of chemical pesticides has caused serious decline in soil fertility, excessive heavy metals, and damage to ecological balance. Therefore, using biological control, the strategy of "using bacteria to control bacteria" is widely recognized and has become a research hotspot at home and abroad. [0003] Trichoderma has the characteristics of fast growth, wide distribution and strong environmental adaptability, and has antagonistic effects on many plant pathogens. Since Weindling first discovered in 1932 that Trichoderma lig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P3/00C12R1/885
CPCC12N1/14C12N1/145C12R2001/885
Inventor 庄文颖张意陈凯
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI