Pruns cistena tissue culture seedling cultivation method

Abstract

The invention discloses a pruns cistena tissue culture seedling cultivation method, relates to plant propagation and belongs to seedling production technology in forestry biotechnology. The method ischaracterized by including: fumigating an intelligent seedling culture greenhouse for disinfection, selecting pruns cistena stems, cutting the same into apical-bud-containing stems of 2-3cm after surface sterilization, putting the stems onto a reciprocating shaking seedbed, and using an adventitious bud culture medium added with 6-BA (1.0mg/l) and NAA (0.05mg/l) for culture; when plants grow to 2.5-3.5cm, transferring onto another half-reciprocating shaking seedbed, using a rooting culture medium added with IBA (1.0mg/l) and IAA (1.0mg/l) to induce rooting, hardening seedlings for 5d after 35d, and transferring the seedlings into a nutrition bowl for propagation. Conventional seedling culture and tissue culture seedling culture methods are combined together, and shallow shake culture is performed through the reciprocating shaking seedbed, the weakness that liquid culture media are prone to causing culture death due to oxygen deficiency and the weakness that solid culture media are slowin growth are avoided, and the objective of large-scale quick propagation is achieved; the method is simple in process and easy for popularization.

Description

technical field [0001] The invention relates to the production technology of rapid propagation of seedlings in the forestry biotechnology, in particular to a method for cultivating seedlings of the purple leaf cherry group. Background technique [0002] Purple leaf dwarf cherry ( Prunus×cistena N.E.Hansen ex Koehne ) belongs to Rosaceae Prunus deciduous small trees or shrubs, plant height 1.5-2.6m, crown width 1.2-2.9m, young branches are purple-brown, usually glabrous, old branches have lenticels, distributed throughout the branches, single leaves alternate, The leaves are oblong-ovate or ovate-oblong, 4-8cm long, apex acuminate, leaf base broadly cuneate, leaf margins have irregular fine teeth, leaf surface is red or purple, and the top of new leaves is bright purple. The flowers are solitary, small, pale pink, with 5 petals, slightly fragrant, with many stamens and single pistil, and the flowering period is from April to May. [0003] Purple leaf dwarf cherry is a hybri...

AI Technical Summary

Problems solved by technology

[0004] Purple leaf dwarf cherry is generally propagated by grafting and cuttings, cutting grafting in spring and autumn or budding in summer and autumn, and mass propagation is very inconvenient

Moreover, because the seedlings of purple-leaved dwarf cherry are relatively slow, they can be applied to projects at least three years, and it takes four to five years for them to grow. Obviously, traditional grafting and cutting propagation methods are far from being able to meet large-scale landscaping projects. construction needs