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Mesoporous silica drug-loaded nanoparticle and preparation method and application thereof

A technology of mesoporous silica and drug-loaded nanometers is applied in the field of medicine to achieve good photothermal treatment effect, good anti-tumor effect, and feasible and reliable operation.

Active Publication Date: 2020-01-17
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, using PDA as a reaction platform to construct a mesoporous silica drug-loading system (MSNs@PDA-PEOz-iRGD) co-modified by the tumor-directed penetration peptide iRGD and the pH-responsive material PEOz, is expected to achieve tumor penetration, triggered release and photothermal The purpose of treatment, the current MSNs@PDA-PEOz-iRGD system and its application in the field of tumor treatment have not been reported

Method used

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  • Mesoporous silica drug-loaded nanoparticle and preparation method and application thereof
  • Mesoporous silica drug-loaded nanoparticle and preparation method and application thereof
  • Mesoporous silica drug-loaded nanoparticle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A preparation method of mesoporous silica drug-loaded nanoparticles, specifically implemented according to the following steps:

[0052] Step 1, Synthesis of MSN

[0053] Weigh 250 mg of CTAB and dissolve it in 120 mL of deionized aqueous solution, then add 875 μL of 2M NaOH, and stir vigorously at 60°C for 30 min. After CTAB is completely dissolved, slowly add 1.25mL TEOS and continue to stir for 2h. After the reaction, the product was left to cool down to room temperature, centrifuged at 10000rpm for 5min, washed three times with deionized water and ethanol respectively, and finally extracted and refluxed with an ethanol solution containing ammonium nitrate (4g / L) for 24h to remove the template agent. After obtaining the sample, Washed three times with deionized water and absolute ethanol respectively, dried in a vacuum oven at 60°C for 12 hours, and marked as MSN.

[0054] Step 2, MSN-NH 2 Synthesis

[0055] Weigh 200mg of MSN powder into a 500mL round bottom fla...

Embodiment 2

[0063] A preparation method of mesoporous silica drug-loaded nanoparticles, specifically implemented according to the following steps:

[0064] Step 1, Synthesis of MSN

[0065]Weigh 300 mg of CTAB and dissolve it in 150 mL of deionized aqueous solution, then add 1000 μL of 2M NaOH, and stir vigorously at 70°C for 40 min. After CTAB was completely dissolved, 1.5mL TEOS was added slowly, and stirring was continued for 3h. After the reaction, the product was cooled to room temperature, centrifuged at 12000 rpm for 8 min, and washed four times with deionized water and ethanol respectively. Finally, the ethanol solution containing ammonium nitrate (5g / L) was extracted and refluxed for 36h to remove the template agent. After the sample was obtained, it was washed 4 times with deionized water and absolute ethanol, and dried in a vacuum oven at 70°C for 12h, marked as MSN .

[0066] Step 2, MSN-NH 2 Synthesis

[0067] Weigh 300mg of MSN powder into a 1000mL round bottom flask, a...

Embodiment 3

[0075] A preparation method of mesoporous silica drug-loaded nanoparticles, specifically implemented according to the following steps:

[0076] Step 1, Synthesis of MSN

[0077] Weigh 400 mg of CTAB and dissolve it in 200 mL of deionized aqueous solution, then add 1200 μL of 2M NaOH, and stir vigorously at 80 °C for 45 min. After CTAB is completely dissolved, slowly add 2.0mL TEOS and continue to stir for 3h. After the reaction, the product was cooled to room temperature, centrifuged at 15,000 rpm for 10 min, and washed five times with deionized water and ethanol respectively. Finally, the ethanol solution containing ammonium nitrate (6g / L) was extracted and refluxed for 48 hours to remove the template agent. After the sample was obtained, it was washed 5 times with deionized water and absolute ethanol, and dried in a vacuum oven at 80°C for 24 hours, marked as MSN .

[0078] Step 2, MSN-NH 2 Synthesis

[0079] Weigh 250mg of MSN powder into a 500mL round bottom flask, ad...

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Abstract

The invention discloses a mesoporous silica drug-loaded nanoparticle. Mesoporous silica is used as a drug carrier, and is wrapped with a polydopamine layer; through a Schiff base addition reaction, the raw material is connected to tumor-directed osmotic peptide iRGD and poly (2-ethyl-2-oxazoline) so as to obtain the mesoporous silica drug-loaded nanoparticle MSN (at) PDA-PEOz-iRGD. The invention also discloses a preparation method of the above drug-loaded nanoparticle. By loading the prepared drug-loaded nanoparticle on an anti-cancer drug, the drug has obvious pH-responsive release, obvious tumor cell targeting ability and good photothermal treatment effect and good antitumor effect in vivo and in vitro, and provides theoretical basis for the research of novel targeted drug delivery system.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a drug-loaded nanoparticle of mesoporous silicon dioxide, and also relates to a preparation method and application of the drug-loaded nanoparticle. Background technique [0002] Malignant tumors seriously threaten human health and life safety. Chemotherapy (chemotherapy) is one of the important means to treat cancer clinically. The nano-delivery system of anti-tumor drugs has become a hot spot in the research of chemotherapeutic drug preparations due to its small size, which can significantly improve the solubility of chemotherapeutic drugs, improve the stability of drugs, and prolong their blood circulation time. Among them, mesoporous silica has been proven to be one of the most promising nanocarriers for drug delivery. The pore size of mesoporous silica is between 2-50nm. It has the advantages of large specific surface area, high drug loading capacity, good bioc...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/04A61K47/42A61K47/34A61K31/337A61K31/704A61K41/00A61P35/00
CPCA61K9/5115A61K9/5169A61K9/5146A61K9/5192A61K31/337A61K31/704A61K41/0052A61P35/00A61K2300/00
Inventor 王小宁梁晓燕闫梦茹马远涛高迎春赵宁
Owner XIAN MEDICAL UNIV
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