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Production method of isopropyl-beta-D-thiogalactopyranoside

A technology of thiogalactopyranose and thiogalactopyranoside, which is applied in the production field of isopropyl-beta-D-thiogalactopyranoside, can solve the problems of strong odor and discomfort, and achieve the effect of easy production

Pending Publication Date: 2020-01-17
湖南汇百益新材料有限公司
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  • Application Information

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Problems solved by technology

The IPTG products produced by it have a very strong odor due to isopropyl mercaptan. Even a small amount of leakage will cause a strong odor in the production area and the surrounding 2km range, causing discomfort to the human body

Method used

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  • Production method of isopropyl-beta-D-thiogalactopyranoside

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Embodiment Construction

[0016] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0017] see figure 1 , the invention provides a kind of production method of isopropyl-beta-D-thiogalactopyranoside (CAPS), the method comprises the steps:

[0018] Step S1. Dissolve 13 g of 2-bromopropane in 200 g of methylene chloride by weight, then add 8 g of thiourea, keep warm at 30° C., and react for 5 hours.

[0019] In this step, 2-bromopropane and thiourea are used to replace isopropyl mercaptan in related production methods. Isopropyl mercaptan has ...

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Abstract

The invention discloses a production method of isopropyl-beta-D-thiogalactopyranoside. The method includes the following steps: dissolving 13g of 2-bromopropane in 200g of dichloromethane according toweight ratio, adding 8g of thiourea, keeping the temperature at 30 DEG C, and reacting for 5 hours; adding 40g of beta-D-galactose pentaacetate, and reducing the temperature to 5-10 DEG C; adding 20gof concentrated sulfuric acid at a preset speed, keeping heat preservation reaction for N hours after the addition, adding clear water for washing, and then using a sodium bicarbonate solution for washing to neutrality; and drying with anhydrous sodium sulfate, filtering, and preforming alcoholysis to obtain the isopropyl-beta-D-thiogalactopyranoside. Compared with the related technology, the production method of isopropyl-beta-D-thiogalactopyranoside has no peculiar smell and is simple and easy to obtain.

Description

technical field [0001] The invention relates to the technical field of chemical production, in particular to a production method of isopropyl-beta-D-thiogalactopyranoside. Background technique [0002] IPTG, the Chinese name isopropyl-beta-D-thiogalactopyranoside, is a very strong inducer, which is not metabolized by bacteria and is very stable, so it is widely used in laboratories. IPTG is commonly used in cloning experiments that require induction of β-galactosidase activity. It is often used in combination with X-Gal or Bluo-Gal for blue-white screening of recombinant bacterial colonies that induce the expression of the lac operon in E. coli. IPTG acts by binding to the lacI repressor protein and changing its conformation, preventing repression of the β-galactosidase-encoding gene lacZ. [0003] In the production of IPTG in the related art, dissolve 40g of β-D-galactose pentaacetate in 200g of dichloromethane, add 40g of boron trifluoride ether, cool down to about 5-10 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/00C07H15/14
CPCC07H1/00C07H15/14
Inventor 肖光汉徐宁
Owner 湖南汇百益新材料有限公司
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