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Liquid fermentation method of trichoderma harzianum for preventing and controlling potato root rot and preparation method of preparation

A technology for liquid fermentation and Trichoderma harzianum, which is applied in the field of bioengineering, can solve problems such as the lack of obvious effects on plant disease control and production increase promotion, and the lack of bio-control Trichoderma vigor, so as to accelerate the sustainable development of agriculture, enrich the types of preparations, easy to achieve effect

Inactive Publication Date: 2020-01-17
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Patent CN200810154241.6 discloses a production process of using Chinese herbal medicine dregs to ferment biocontrol Trichoderma, using traditional Chinese medicine dregs in the pharmaceutical process of traditional Chinese medicine factory as a part of the culture medium to ferment and produce biocontrol Trichoderma. Both of the above two patents are It does not explain how the activity of the bio-control Trichoderma is obtained, and solve the problem of the production of Trichoderma in the fermentation process, and how effective it is in production, and the effect on the prevention and control of related plant diseases and the promotion of production increase is not obvious

Method used

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  • Liquid fermentation method of trichoderma harzianum for preventing and controlling potato root rot and preparation method of preparation

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Embodiment 1

[0040] The present embodiment provides a kind of Trichoderma harzianum liquid fermentation method and preparation method thereof for preventing and controlling potato root rot, comprising the following steps:

[0041] Step 1, culture on a slant, inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and cultivate it at 25°C for 7 days under dark conditions;

[0042] Step 2, seed liquid culture: inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 15g / L of sucrose, 3g / L of yeast powder, 0.01g / L of NaCl, FeSO 4 ·7H 2 O 0.1g / L, MgSO 4 ·7H 2 O 1.2g / L, KH 2 PO 4 3.5g / L, NaNO 3 6.5g / L; the medium is sterilized by moist heat at 121°C for 25 minutes; the inoculum size is 1cm 2 Inclined spores; the culture conditions were cultured in a shaker at 25°C for 6 days in the dark, with a shaker speed of 180rpm;

[0043] Step 3, liquid fermentation: inoculate the seed liquid cultivated in step 2 into...

Embodiment 2

[0049] The present embodiment provides a kind of Trichoderma harzianum liquid fermentation method and preparation method thereof for preventing and controlling potato root rot, comprising the following steps:

[0050] Step 1, culture on a slant, inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and culture at 2°C for 6 days under dark conditions;

[0051] Step 2, seed liquid culture: inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 16g / L of sucrose, 4g / L of yeast powder, 0.02g / L of NaCl, FeSO 4 ·7H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1.3g / L, KH 2 PO 4 3.8g / L, NaNO 3 6.6g / L; the medium is sterilized by moist heat at 121°C for 28 minutes; the inoculum size is 1cm 2 Inclined spores; the culture conditions were cultured in a shaker at 25°C for 5 days in the dark, with a shaker speed of 190rpm;

[0052] Step 3, liquid fermentation: inoculate the seed liquid cultivated in step 2 into a ste...

Embodiment 3

[0057] The present embodiment provides a kind of Trichoderma harzianum liquid fermentation method and preparation method thereof for preventing and controlling potato root rot, comprising the following steps:

[0058] Step 1, culture on a slant, inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and culture at 27°C for 6 days under dark conditions;

[0059] Step 2, seed liquid culture: inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 20g / L of sucrose, 4g / L of yeast powder, 0.03g / L of NaCl, FeSO 4 ·7H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1.4g / L, KH 2 PO 4 4.0g / L, NaNO 3 6.7g / L; the medium is sterilized by moist heat at 121°C for 30 minutes; the inoculum size is 1.5cm 2 Inclined spores; the culture conditions were cultured in a shaker at 27°C for 4 days in the dark, with a shaker speed of 200rpm;

[0060] Step 3, liquid fermentation: inoculate the seed liquid cultivated in step 2 into a ...

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Abstract

The invention relates to a liquid fermentation method of trichoderma harzianum for preventing and controlling potato root rot and a preparation method of a preparation. The method comprises the stepsof inclined surface culture, seed liquid culture, liquid fermentation and preparation preparing. The dosage form of the prepared preparation is a liquid preparation or wettable powder. Traditional Chinese medicine extracts are added into a liquid fermentation culture medium; after the fermentation is completed or a fermentation product is directly treated, packaging is performed to obtain the liquid preparation; or drying separation is performed to obtain trichoderma harzianum mycelia; then, traditional Chinese medicine residues after leaching extraction are crushed after being dried, and areused as carriers to be mixed with the mycelia so as to prepare a solid preparation; and the liquid preparation or the solid preparation has good inhibition effects on potato root rot pathogenic bacteria such as fusarium equiseti, fusarium sambucinum, fusarium oxysporum, fusarium solani and fusarium acuminatum, and can be applied to the prevention and control process of the potato root rot so as toreduce, prevent and control the occurrence of the potato root rot and improve the yield and the quality of the potatoes.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a liquid fermentation method of Trichoderma harzianum for preventing and controlling potato root rot and a preparation method of a preparation. Background technique [0002] As a biocontrol fungus, Trichoderma harzianum can be used to prevent plant diseases caused by pathogens such as Pythium, Rhizoctonia solani, Fusarium, Rhizopus niger, Cylindrosporium, Sclerotinia, and Sclerotinia. Among them, root rot, dry rot, and fusarium wilt caused by Fusarium infection of potatoes are worldwide soil-borne fungal diseases. Potato root rot can occur during the seedling and field growth stages. Potato fusarium root rot has caused serious damage to potato production in recent years. Generally, the affected plots have reduced production by 13% to 35%, and serious slices of them have died or even ceased production. Potato root rot is generally treated with chemical fungicides. There ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14
Inventor 王喜刚郭成瑾沈瑞清焦杨张丽荣杨波
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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