Liquid fermentation method of trichoderma harzianum for preventing and controlling potato root rot and preparation method of preparation
A technology for liquid fermentation and Trichoderma harzianum, which is applied in the field of bioengineering, can solve problems such as the lack of obvious effects on plant disease control and production increase promotion, and the lack of bio-control Trichoderma vigor, so as to accelerate the sustainable development of agriculture, enrich the types of preparations, easy to achieve effect
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Embodiment 1
[0040] The present embodiment provides a kind of Trichoderma harzianum liquid fermentation method and preparation method thereof for preventing and controlling potato root rot, comprising the following steps:
[0041] Step 1, culture on a slant, inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and cultivate it at 25°C for 7 days under dark conditions;
[0042] Step 2, seed liquid culture: inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 15g / L of sucrose, 3g / L of yeast powder, 0.01g / L of NaCl, FeSO 4 ·7H 2 O 0.1g / L, MgSO 4 ·7H 2 O 1.2g / L, KH 2 PO 4 3.5g / L, NaNO 3 6.5g / L; the medium is sterilized by moist heat at 121°C for 25 minutes; the inoculum size is 1cm 2 Inclined spores; the culture conditions were cultured in a shaker at 25°C for 6 days in the dark, with a shaker speed of 180rpm;
[0043] Step 3, liquid fermentation: inoculate the seed liquid cultivated in step 2 into...
Embodiment 2
[0049] The present embodiment provides a kind of Trichoderma harzianum liquid fermentation method and preparation method thereof for preventing and controlling potato root rot, comprising the following steps:
[0050] Step 1, culture on a slant, inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and culture at 2°C for 6 days under dark conditions;
[0051] Step 2, seed liquid culture: inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 16g / L of sucrose, 4g / L of yeast powder, 0.02g / L of NaCl, FeSO 4 ·7H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1.3g / L, KH 2 PO 4 3.8g / L, NaNO 3 6.6g / L; the medium is sterilized by moist heat at 121°C for 28 minutes; the inoculum size is 1cm 2 Inclined spores; the culture conditions were cultured in a shaker at 25°C for 5 days in the dark, with a shaker speed of 190rpm;
[0052] Step 3, liquid fermentation: inoculate the seed liquid cultivated in step 2 into a ste...
Embodiment 3
[0057] The present embodiment provides a kind of Trichoderma harzianum liquid fermentation method and preparation method thereof for preventing and controlling potato root rot, comprising the following steps:
[0058] Step 1, culture on a slant, inoculate the M-17 strain of Trichoderma harzianum on the slant of the PDA medium, and culture at 27°C for 6 days under dark conditions;
[0059] Step 2, seed liquid culture: inoculate the bacteria activated in step 1 into the sterilized seed medium for cultivation, the seed medium is 20g / L of sucrose, 4g / L of yeast powder, 0.03g / L of NaCl, FeSO 4 ·7H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1.4g / L, KH 2 PO 4 4.0g / L, NaNO 3 6.7g / L; the medium is sterilized by moist heat at 121°C for 30 minutes; the inoculum size is 1.5cm 2 Inclined spores; the culture conditions were cultured in a shaker at 27°C for 4 days in the dark, with a shaker speed of 200rpm;
[0060] Step 3, liquid fermentation: inoculate the seed liquid cultivated in step 2 into a ...
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