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A method for enhancing the luminescence intensity of squamous cell carcinoma antigen in electrochemiluminescence detection

A squamous cell carcinoma and luminescent detection technology, which is applied in the direction of chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, and measurement devices, can solve the problems of unfavorable rapid analysis, tedious, high-tech requirements, etc., and achieve improvement Electrochemiluminescent intensity, accelerated electron transfer rate, and good selectivity

Active Publication Date: 2021-04-30
GUANGXI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the existing analysis methods, some methods require expensive experimental equipment and high technical requirements, while others are more cumbersome, which is not conducive to rapid analysis
However, there is no Fe-MIL-88-NH 2 Related reports on the construction of electrochemiluminescent sensors by combining metal-organic frameworks loaded with ZnSe quantum dots and electrodeposition molecular imprinting technology to enhance the luminescence intensity of squamous cell carcinoma antigens in electrochemiluminescent detection

Method used

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  • A method for enhancing the luminescence intensity of squamous cell carcinoma antigen in electrochemiluminescence detection
  • A method for enhancing the luminescence intensity of squamous cell carcinoma antigen in electrochemiluminescence detection
  • A method for enhancing the luminescence intensity of squamous cell carcinoma antigen in electrochemiluminescence detection

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Experimental program
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Embodiment 1

[0035] 1. Experimental method

[0036] 1.1 Reagents

[0037] Selenium powder (Aladdin Reagent Company); Sodium borohydride (Shanghai Tianlian Fine Chemical Co., Ltd.); 3-mercaptopropionic acid (MPA), 1-ethyl-3-(3-dimethylaminopropylcarbotriimide) ( EDC), polyvinylpyrrolidone (PVP), FeCl 3 ·6H 2 O, 2-aminoterephthalic acid (2-NH 2 -BDC), dopamine (DA) (Aladdin reagent company), zinc chloride, sodium hydroxide, disodium hydrogen phosphate, sodium dihydrogen phosphate, N,N-dimethylformamide (DMF), ethanol (Shantou West, Guangdong Long Chemical Industry Co., Ltd.); sodium lauryl sulfate, bovine serum albumin (BSA) (Beijing Suo Laibao Technology Co., Ltd.); acetic acid (Guangdong Guanghua Technology Co., Ltd.); squamous cell carcinoma antigen (SCCA), mouse anti-SCCA antibody, alpha-fetoprotein (AFP), CA15-3, carcinoembryonic antigen (CEA) (Beijing Boaosen Biotechnology Co., Ltd.).

[0038] 1.2 Instruments

[0039] MPI-B multi-parameter chemical analysis detection system (Xi'a...

experiment example 1

[0072] Experimental Example 1: The method of the present invention is applied to the analysis of actual specimen samples In order to investigate whether the electrochemiluminescence sensor constructed by the present invention can be used for the analysis of complex samples, the applicant used the sensor to analyze human serum samples. The human serum samples came from the Fifth People’s Hospital of Guilin City, and were directly analyzed according to the above-mentioned immunoassay method without any pretreatment. The RSD is less than 7%, indicating that the electrochemiluminescent sensor constructed in the present invention is suitable for complex biological sample analysis.

[0073] Table 2: Determination results and recovery rates of SCCA in human serum samples (n=3)

[0074]

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Abstract

The invention discloses a method for enhancing the luminescence intensity of squamous cell carcinoma antigen in electrochemiluminescence detection, which mainly includes the construction step of electrochemiluminescence sensor. Add dropwise to the polymer molecularly imprinted modified electrode for incubation, wash with water after incubation, and then add Fe‑MIL‑88‑NH dropwise on the electrode 2 @ZnSe / Ab / BSA complex and incubate, wash with water after incubation, and dry to form an electrochemiluminescence sensor; use the electrochemiluminescence sensor prepared above to place in PBS buffer containing co-reactants for electrochemiluminescence Detection can effectively enhance the luminescence intensity of SCCA in electrochemiluminescence detection. The electrochemiluminescence sensor constructed by the invention has good selectivity and high sensitivity, and when it is used for electrochemiluminescence detection of SCCA, the linear range of the method is effectively increased and the detection sensitivity is improved.

Description

technical field [0001] The invention relates to a method for enhancing the luminescence intensity of a squamous cell carcinoma antigen during electrochemiluminescence detection, and belongs to the technical field of biological detection. Background technique [0002] Cervical cancer is the most common female cancer worldwide. Squamous cell carcinoma antigen (SCCA) is a subtype of glycoprotein with a molecular weight of about 45 to 55 kDa, which was isolated from cervical squamous cell carcinoma tissue for the first time (Wu L, Hu Y F, Sha Y H, Li W R, Yan T T, Wang S, Li X, Guo Z Y, Zhou J, Su XR. An “in-electrode”-type immunosensing strategy for the detection of squamouscell carcinoma antigen based on electrochemiluminescent AuNPs / g-C 3 N 4 nanocomposites [J]. Talanta, 2016, 160:247-255.). Initially, SCCA was found to be a good specific antigen for the diagnosis and monitoring of cervical squamous cell carcinoma. Later, SCCA was found to be associated with other types o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N21/76
CPCG01N21/76G01N27/308G01N27/3277G01N27/3278
Inventor 邓必阳莫桂春郑向菲冯金素
Owner GUANGXI NORMAL UNIV
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