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Preparation method and application of an electrochemiluminescent bisphenol A biosensor based on nitrogen-doped titanium dioxide nanosheets

A biosensor, titanium dioxide technology, applied in the direction of chemiluminescence/bioluminescence, analysis through chemical reaction of materials, scientific instruments, etc., can solve the problems of low sensitivity, unfavorable practical application, and poor conductivity of electrochemiluminescence sensors , to achieve the effects of increasing the intensity of electrochemiluminescence, improving the ability of resonance energy transfer, and improving the intensity and stability

Inactive Publication Date: 2018-03-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the poor conductivity of titanium dioxide also limits the sensitivity of electrochemiluminescence sensors constructed from a single titanium dioxide nanomaterial, which is not conducive to practical applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 FeMn-N@TiO 2 preparation of

[0038] First, add 0.8 mmol of iron salt, 0.8 mmol of manganese salt and 1 mmol of ammonium salt into 5 mL of tetrabutyl titanate, slowly add 0.5 mL of hydrofluoric acid during stirring, and react in the reactor at 160 °C for 24 hours , cooled to room temperature, centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50 °C; then, the ground powder was put into a muffle furnace with a heating rate of 1 °C / min at 480 °C Calcined for 60 min; finally, the calcined powder was cooled to room temperature to obtain FeMn-N@TiO 2 ;

[0039] Described iron salt is ferric sulfate;

[0040] Described manganese salt is manganese sulfate;

[0041] Described ammonium salt is ammonium sulfate.

Embodiment 2

[0042] Example 2 FeMn-N@TiO 2 preparation of

[0043] First, add 0.8 mmol of iron salt, 1.0 mmol of manganese salt and 1 mmol of ammonium salt into 5 mL of tetrabutyl titanate, slowly add 0.65 mL of hydrofluoric acid during stirring, and react in the reactor at 180 °C for 21 hours , cooled to room temperature, centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50 °C; then, the ground powder was put into a muffle furnace with a heating rate of 2 °C / min, at 520 °C Calcined for 30 min; finally, the calcined powder was cooled to room temperature to obtain FeMn-N@TiO 2 ;

[0044] Described iron salt is ferric chloride;

[0045] Described manganese salt is manganese chloride;

[0046] Described ammonium salt is ammonium chloride.

Embodiment 3

[0047] Example 3 FeMn-N@TiO 2 preparation of

[0048] First, add 0.8 mmol of iron salt, 1.2 mmol of manganese salt and 1 mmol of ammonium salt into 5 mL of tetrabutyl titanate, slowly add 0.8 mL of hydrofluoric acid during stirring, and react in the reactor at 200 °C for 18 hours , after cooling to room temperature, centrifuged and washed three times with ultrapure water and absolute ethanol, and then vacuum-dried at 50°C; then, the ground powder was put into a muffle furnace with a heating rate of 3°C / min, at 560°C Calcined for 10 min; finally, the calcined powder was cooled to room temperature to obtain FeMn-N@TiO 2 ;

[0049] Described iron salt is ferric nitrate;

[0050] Described manganese salt is manganese nitrate;

[0051] Described ammonium salt is ammonium nitrate.

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Abstract

The invention discloses a preparation method of an electrochemiluminescent bisphenol A biosensor based on nitrogen-doped titanium dioxide nanosheets. It belongs to the technical field of new nanometer functional materials and biosensors. The present invention firstly prepares a novel sheet-shaped magnetic nano-photosensitive material FeMn-N@TiO2, utilizes the good biocompatibility, large specific surface area and ferromagnetism of the material, loads bisphenol A antibody, and then The cross-linking effect of dialdehyde horseradish peroxidase, when detecting, because horseradish peroxidase can catalyze hydrogen peroxide to generate oxygen in situ, and then electrochemically react with the bottom liquid to produce high and stable Electrochemiluminescent signal, and then use the effect of the specific quantitative binding of antibody and antigen on the electron transport ability, so that the electrochemiluminescent signal is reduced accordingly, and finally a low-cost, high-sensitivity, good-specificity, rapid detection, preparation A simple electrochemiluminescent biosensor for the detection of bisphenol A.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescent bisphenol A biosensor. It belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Environmental estrogen refers to a class of compounds that interfere with the synthesis, release, transport, binding, and metabolism of normal endocrine substances in the body after entering the body, activate or inhibit the function of the endocrine system, and thereby destroy the stability and regulation of the body. There are many kinds of environmental estrogens, including synthetic compounds and plant natural estrogens, which are widely distributed in nature. Bisphenol A is a bisphenol-like synthetic estrogen. [0003] At present, the methods for detecting bisphenol A mainly include chromatography and mass spectrometry. Such methods require expensive instruments and complex operations, and laboratory personnel need professional tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N33/543
CPCG01N21/76G01N33/5438
Inventor 张勇吴丹王耀光范大伟魏琴
Owner UNIV OF JINAN
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