Micro-fluidic chip for isolating and capturing single cell and application of micro-fluidic chip

Abstract

The embodiment of the invention provides a micro-fluidic chip for isolating and capturing a single cell. The micro-fluidic chip comprises a chip substrate; the chip substrate is provided with a sampleinjection hole and a plurality of sampling holes; the sampling injection hole is located in the center of the chip substrate, wherein the sample injection hole communicates with at least two groups of multistage flow channels, each group of multistage flow channel comprises a main flow channel, a first branch flow channel and a plurality of second branch flow channels; one end of the main flow channel communicates with the sample injection hole, and the other end of the main flow channel communicates with the first branch flow channel; and the plurality of second branch flow channel are symmetrically arranged at two sides of the first branch flow channel, one end of each of the plurality of second branch flow channels communicates with the first branch flow channel, the other end of eachof the second branch flow channels is connected with at least one of the sampling holes, and each of the sampling holes communicates with one of the second branch flow channels. By using the micro-fluidic chip, the single chip can be rapidly and simply isolated and captured with low cost and low threshold. The invention further provides a preparation method and application of the micro-fluidic chip.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip for separating and capturing single cells, a preparation method and application thereof. Background technique [0002] Studies in recent years have found that the study of cell biology of a single cell is of great significance. It can reveal the gene structure and gene expression status of a single cell, reflect the heterogeneity between cells, and be useful in the early diagnosis of major diseases, tumors, and development. Biology, microbiology, neuroscience and other fields play an important role and are becoming the focus of life science research. Single-cell separation and capture technology is the basis for research at the single-cell level. At present, the methods that can achieve single cell sorting mainly focus on flow cytometry or single cell sorting instrument. Flow cytometry can achieve multi-channel sorting of living cells; single cell sort...

AI Technical Summary

Problems solved by technology

However, the existing flow cytometer is expensive, bulky, and requires special personnel to operate, so it cannot be popularized in a large area

Single-cell sorters are also expensive, time-consuming and labor-intensive to operate, and require high technology for operators

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