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Method for measuring content of ferulylphenethylamine in C. f r u t e s c e n s, and quality detection method

A technology of feruloyl phenethylamine and feruloyl p-hydroxyphenylethylamine, which is applied in the field of chromatographic detection, can solve the problems affecting the safety of clinical medication of jiu feng, the development and utilization of jiu feng, and the complexity of components, and achieves good results. Specificity, fast speed, good effect

Active Publication Date: 2020-01-21
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although nine layers of wind are recorded in the first volume of "Flora of China" and "Quality Standards of Zhuang Medicine of Guangxi Zhuang Autonomous Region", but there is no content related to the determination of its standard content
[0003] The components in Jiu Layer Feng are complex, and the contents of Jiu Layer Feng cultivated in different regions and under different environmental conditions are different. Although Jiu Layer Feng has been used in traditional Chinese medicine for a long time, the amount of Jiu Layer Feng used The guidance is based on the weight of the dried stems and branches of the nine-layer wind, which will not only affect the development and utilization of the nine-layer wind, but also affect the clinical drug safety of the nine-layer wind

Method used

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  • Method for measuring content of ferulylphenethylamine in C. f r u t e s c e n s, and quality detection method
  • Method for measuring content of ferulylphenethylamine in C. f r u t e s c e n s, and quality detection method
  • Method for measuring content of ferulylphenethylamine in C. f r u t e s c e n s, and quality detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Obtain the linear equation of N-trans-feruloyl p-hydroxyphenethylamine reference substance

[0047] S1. Take 0.2, 0.4, 0.8, 1.6, and 2.4 mL of N-trans-feruloyl p-hydroxyphenethylamine reference substance solution with a mass concentration of 1.0 mg / mL, place them in 10 mL volumetric flasks, and add methanol The solution was dissolved and diluted to the mark and shaken evenly to obtain N-trans-feruloyl p-hydroxyphenethylamine reference substance solutions with different concentrations.

[0048] S2, carry out chromatographic analysis to the N-trans feruloyl p-hydroxyphenylethylamine reference substance solution of different concentrations respectively, and record the chromatogram of the N-trans feruloyl p-hydroxyphenethylamine reference substance solution of different concentrations obtained Peak area. Chromatographic conditions: chromatographic column: 4.6×250mm, 5μm particle size, Agilent TC-C18 chromatographic column; detection wavelength: 317nm; injection volume: ...

Embodiment 2

[0061] One, investigate the specificity of the chromatographic conditions in embodiment 1

[0062] Accurately draw N-trans-feruloyl p-hydroxyphenethylamine reference substance solution, test solution, and negative control solution respectively 10 μ L, inject liquid chromatograph, use the chromatographic conditions in the embodiment 1 to carry out chromatographic analysis, the results are shown in Figure 5-7 As shown, the results show that: N-trans-feruloyl p-hydroxyphenethylamine in the nine-layer wind test product is consistent with the retention time of the N-trans-feruloyl p-hydroxyphenethylamine reference substance chromatographic peak, and coexists with other Component separation degree is 1.58 (greater than 1.5), and theoretical plate number is 7798 (greater than 3000), and main peak and impurity peak reach baseline separation, and negative control sample does not have chromatographic peak interference at corresponding position, illustrates that in the nine-layer wind of...

Embodiment 3

[0092] 1. Obtain the linear equation of N-trans-feruloyl-5'-methoxy-6'-hydroxyphenethylamine reference substance

[0093] S1. Take 0.2, 0.4, 0.8 and 1.6 mL of N-trans-feruloyl-5'-methoxy-6'-hydroxyphenethylamine reference substance solution with a mass concentration of 1.0 mg / mL, and put them in In a 10mL volumetric flask, add methanol solution to dissolve and dilute to the mark and shake well to obtain different concentrations of N-trans-feruloyl-5'-methoxy-6'-hydroxyphenethylamine reference substance solutions.

[0094] S2. Perform chromatographic analysis on different concentrations of N-trans-feruloyl-5'-methoxy-6'-hydroxyphenethylamine reference solution respectively, and record the obtained N-trans-feruloyl in different concentrations -The chromatographic peak area of ​​the 5'-methoxy-6'-hydroxyphenethylamine reference substance solution. Chromatographic conditions: chromatographic column: 4.6×250mm, 5μm particle size, Agilent TC-C18 chromatographic column; detection wave...

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Abstract

The invention discloses a method for measuring the content of ferulylphenethylamine in C. f r u t e s c e n s. The method comprises the steps of performing chromatographic analysis on a reference substance solution of ferulylphenethylamine, and obtaining a linear equation of peak area and concentration; after a to-be-tested sample solution is prepared by using C. f r u t e s c e n s, performing chromatographic analysis to obtain a peak area of a to-be-tested sample, calculating the concentration of the to-be-tested sample according to the linear equation, and finally obtaining the content of the ferulylphenylethylamine in the C. f r u t e s c e n s. By adopting the method for quickly and accurately measuring the content of the ferulylphenethylamine in the C. f r u t e s c e n s disclosed by the invention, a foundation is laid for the quality control and the research and development of Zhuang medicine ethnodrug.

Description

technical field [0001] The invention belongs to the technical field of chromatographic detection, and in particular relates to a method for determining the content of feruloylphenethylamine in nine-layer wind and a method for using it as a quality detection method for nine-layer wind. Background technique [0002] C.frutescens (C.frutescens) is the dry stem of Cladostachysamaranthoides (Lam.) Kua., a plant of the genus Amaranthus in the family Amaranthaceae. It is a commonly used authentic medicinal material in Guangxi. [1] , is widely used in Guangxi Zhuang medicine ethnic medicine. It has the functions of clearing water, valley road, regulating fire, removing wind toxin, and dehumidification. Diarrhea), ayimi (dysentery) and other diseases [2] . It is also commonly used in the folk to prepare medicinal wine to treat rheumatoid arthritis. Although Jiucaofeng is recorded in the first volume of "Flora of China" and "Quality Standards of Zhuang Medicine of Guangxi Zhuang Au...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/86Y02A50/30
Inventor 姚彩云宋姗珊闫炳雄宋志军刘喜慧
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS