Functional mixed feed additive and preparation method thereof
A technology for mixing feed and additives, applied in the field of feed additives, can solve the problems of difficult absorption and utilization of hemicellulose, affecting the range of corncob feed utilization, etc., and achieve high market prospects and economic benefits, low cost and good effect. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] Preparation of Cordyceps militaris solution
[0037] Boil 120g of potatoes, 5g of silkworm chrysalis powder, and 5g of malt for 30 minutes to filter the residue to obtain a supernatant liquid, add 10g of glucose, 7.5g of peptone, 6g of yeast extract, 1.2g of potassium dihydrogen phosphate, 1.2g of magnesium sulfate, and 0.03g of vitamin B1, Add water to make up to 1000ml, mix evenly, pH 7.2, and sterilize at 121°C for 30min to obtain Cordyceps militaris seed culture medium.
[0038] Inoculate 3 bacterial blocks with a diameter of about 4mm into a Erlenmeyer flask with a loading capacity of 200mL / 1000mL of seed medium and inoculate Cordyceps militaris in the seed medium, and culture it on a shaker at 165rpm at 26°C in a dark environment for 4 days; The seed liquid is inserted into the fermentation medium according to the volume percentage of 12%, and the rotation speed is 180rpm for 105h; when the pH value is 3.5, the fermentation is stopped to obtain the bacterial liqui...
Embodiment 2
[0041] Preparation of Cordyceps militaris solution
[0042] Boil 120g of potatoes, 5g of silkworm chrysalis powder, and 5g of malt for 30 minutes to filter the residue to obtain a supernatant liquid, add 10g of glucose, 7.5g of peptone, 6g of yeast extract, 1.2g of potassium dihydrogen phosphate, 1.2g of magnesium sulfate, and 0.03g of vitamin B1, Add water to make up to 1000ml, mix evenly, pH 7.2, and sterilize at 121°C for 30min to obtain Cordyceps militaris seed culture medium.
[0043] Inoculate 3 bacterial blocks with a diameter of about 4mm into a Erlenmeyer flask with a loading capacity of 200mL / 1000mL of seed medium and inoculate Cordyceps militaris in the seed medium, and culture it on a shaker at 150rpm at 25°C in a dark environment for 3 days; The seed liquid is inserted into the fermentation medium according to the volume percentage of 10%, and the rotation speed is 160rpm for 96 hours; when the pH value is 3.5, the fermentation is stopped to obtain the bacterial l...
Embodiment 3
[0046] Preparation of Cordyceps militaris solution
[0047] Boil 120g of potatoes, 5g of silkworm chrysalis powder, and 5g of malt for 30 minutes to filter the residue to obtain a supernatant liquid, add 10g of glucose, 7.5g of peptone, 6g of yeast extract, 1.2g of potassium dihydrogen phosphate, 1.2g of magnesium sulfate, and 0.03g of vitamin B1, Add water to make up to 1000ml, mix evenly, pH 7.2, and sterilize at 121°C for 30min to obtain Cordyceps militaris seed culture medium.
[0048] Inoculate 3 bacterial blocks with a diameter of about 4mm into a Erlenmeyer flask with a loading capacity of 200mL / 1000mL of seed medium and inoculate Cordyceps militaris in the seed medium, and culture on a shaker at 180rpm at 25°C in a dark environment for 5 days; The seed liquid is inserted into the fermentation medium according to the volume percentage of 15%, and the rotation speed is 220rpm for 120h; when the pH value is 3.5, the fermentation is stopped to obtain the bacterial liquid o...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More