A method for preparing porous starch by using alternating electric field

A technology of porous starch and alternating electric field, applied in the field of starch processing, can solve the problems of large amount of enzymes and long time.

Active Publication Date: 2021-04-27
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the shortcomings of the existing biological method for preparing porous starch such as long time and large amount of enzyme, the applicant of the present invention provides a method for efficiently preparing porous starch

Method used

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  • A method for preparing porous starch by using alternating electric field
  • A method for preparing porous starch by using alternating electric field
  • A method for preparing porous starch by using alternating electric field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use broken rice flour with a 0.3mm sieve as raw material, potassium phosphate buffer solution with pH 6.0 and conductivity 25.8mS / cm as reaction medium, high temperature resistant α-amylase as catalyst, 80mL buffer solution, 8.0g broken rice flour and After the high-temperature-resistant α-amylase (enzyme activity 20000U / mL) with 1% dry weight of starch was mixed evenly under the action of magnetic stirring, it was treated for 120min under the conditions of electric field strength 5V / cm, frequency 60Hz, temperature 50°C, and the pH was adjusted to 10.0 After inactivating the enzyme overnight, adjust the pH to be neutral, wash with ultrapure water, centrifuge, and freeze-dry to obtain porous starch with different pore structures.

Embodiment 2

[0028] The discarded potato peels and residues from industrially produced potato chips passed through a 0.3mm sieve were used as raw materials, potassium phosphate buffer solution with pH 6.0 and conductivity 12.1mS / cm was used as the reaction medium, and medium-temperature α-amylase (50U / mg) was used as the reaction medium. Catalyst, 80mL of buffer solution, 16.0g of potato waste, and medium-temperature α-amylase with 1% dry weight of starch were mixed evenly under the action of magnetic stirring, and then treated for 30min under the conditions of electric field strength 10V / cm, frequency 50Hz, and temperature 45°C Finally, adjust the pH to 10.0 and inactivate the enzyme overnight, adjust the pH to 7.0-8.0, wash with ultrapure water, centrifuge, and freeze-dry to obtain porous starch with different pore structures.

Embodiment 3

[0030] Common cornstarch passed through a 0.3mm sieve was used as raw material, potassium phosphate buffer solution with pH 6.0 and conductivity 25.8mS / cm was used as reaction medium, and high temperature resistant α-amylase (20000U / mL) and β-amylase (50U / mg) as catalyst, 80mL of buffer solution, 8.0g of cornstarch, and high temperature-resistant α-amylase with 1% dry basis weight of starch were mixed evenly under the condition of 50°C with magnetic stirring, and the After 10 minutes of treatment, 0.5% β-amylase on a dry basis of starch was added, and the treatment was continued for 20 minutes under the conditions of an electric field intensity of 10 V / cm and a frequency of 50 Hz. Afterwards, adjust the pH to 10.0 to inactivate the enzyme, adjust the pH to 7.0-8.0, wash with ultrapure water, centrifuge, and freeze-dry to obtain porous starch with different pore structures.

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Abstract

The invention discloses a method for efficiently preparing porous starch by using an electric field. The method uses starch-based materials as raw materials, uses amylase as a catalyst, and uses phosphate buffer as a reaction medium. Process 10-120min to obtain the described porous starch. The invention uses an alternating electric field to induce a current to generate heat through the starch-enzyme mixture, and has the advantages of fast heating rate and uniform heating compared with traditional water bath heating, and can save 40%-80% of energy.

Description

technical field [0001] The invention belongs to the technical field of starch processing and relates to a method for efficiently preparing porous starch by using an alternating electric field. Background technique [0002] Porous starch is a new type of modified starch, which is mostly prepared by biological methods, that is, using amylase to hydrolyze the surface of starch below the starch gelatinization temperature to produce small pores. Compared with native starch, porous starch has larger porosity, specific surface area and better adsorption performance, and has been widely used in food, environmental protection, agriculture, medical treatment, papermaking, cosmetics and other industries. However, the dense semi-crystalline structure of starch limits its interaction with enzyme reagents. The traditional enzymatic preparation of porous starch usually requires increasing the amount of enzyme or prolonging the reaction time to increase the yield. Alternating electric fiel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/00C12P19/04C12P19/22C12P19/14B01J20/24C02F1/28C08L3/02
CPCB01J20/24C02F1/286C08J9/00C08J2303/02C12P19/04C12P19/14C12P19/22
Inventor 李丹丹蒋黎明韩永斌陶阳侯佳曼邓笳李玟珊杨哪徐学明
Owner NANJING AGRICULTURAL UNIVERSITY
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