Cell microcarrier separating device

A technology of cell microcarrier and separation device, which is applied in the field of cell microcarrier separation device, which can solve the problems that antibody filtration and clarification methods cannot be used, separation filter membranes are clogged, and centrifugation methods cannot achieve ideal separation effects, etc.

Pending Publication Date: 2020-01-31
上海乘黄纳米抗体科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different harvested products, the filtration and clarification method in the antibody production process cannot be used. Centrifugation can be used to simply collect cells. However, when cells need to be separated after co-cultivation with microcarriers, simple centrifugation often cannot achieve the ideal separation effect. And increase the complexity of the process. In addition, the cell density co-cultured with microcarriers is high, which can reach 1×10 7 / ml or more, when the filter method is used to pass through the filter membrane, it is easy to clog the separation filter membrane

Method used

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Embodiment 1

[0055] This embodiment describes a microcarrier separation device of the present invention with reference to the accompanying drawings.

[0056] Such as figure 1 As shown, the separation device includes a tank body 1, a filter 2, a stirring system and a liquid inlet and outlet. The tank body 1 can contain the microcarrier cell mixture to be separated. The tank body 1 has an end cover 11 which can seal the tank body 1 . The tank body 1 is made of stainless steel.

[0057]The filter 2 comprises a baffle support 3 and a vessel frame structure, optionally also comprising a first filter membrane and a second filter membrane. The container frame structure includes a structure forming the upper opening of the container, a filter membrane fixing member 4 formed around the container for fixing the filter membrane connected with the structure forming the upper opening of the container, and a bottom container connected with the fixing member. The material of the retainer supporting m...

Embodiment 2

[0062] This embodiment describes the operation process of using the microcarrier separation device in Embodiment 1 to separate microcarriers and cells with reference to the accompanying drawings.

[0063] When the separation device is working, the microcarrier cell mixture and separation enzyme liquid to be separated are firstly added through the liquid inlet 8 and mixed thoroughly. A filter membrane (with a pore size of 50-70 μm) is placed in both the filter membrane fixing part 4 and the stopper support part 3 of the filter 2, and the filter 2 is fixed on the end cap 11. Cover the end cover 11, turn on the stirring system, the motor 5 drives the stirring paddle 7 to rotate rapidly through the stirring shaft 6, and forms a vortex flow field in the liquid. At this time, the microcarriers and cells outside the filter 2 all flow towards the center of the vortex, and when they flow to the first filter membrane on the filter membrane holder 4 of the filter 2, the cells can normall...

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Abstract

The invention provides a cell microcarrier separating device. The cell microcarrier separating device comprises a tank body, a filter, a stirring system and a liquid inlet and outlet, wherein the filter is mounted in the tank body; a filtering film can be fixed to the filter; the stirring system is used for forming a swirling flow field of which the vortex center is located in the filter in liquid; and after the stirring system is started, a microcarrier and cells outside the filter flow to the vortex center in the filter, when the microcarrier and the cells flow to the filtering film on the filter, the cells of which the dimension is smaller than the aperture of the filtering film can normally pass, and the microcarrier of which the dimension is larger than the aperture of the filtering film is blocked, so that the microcarrier and the cells in the microcarrier-cell mixing fluid can be separated. According to the cell microcarrier separating device disclosed by the invention, the microcarrier and the cells can be separated from the swirling flow field and the filtering film, so that the operation time is low, the branching efficiency is high, and the cell microcarrier separating device is suitable for cellular fluid treatment of various volumes and various scales.

Description

technical field [0001] The invention belongs to the technical field of biological cell engineering, and in particular relates to a cell microcarrier separation device. Background technique [0002] Microcarrier culture is a common technology in the field of cell culture at present. It uses fine particles as carriers (about 100 μm in size) and cell suspension (about 10 μm in size) to fully mix, and the cells can attach to the surface of the carrier for culture and proliferation. A cell culture technique. Microcarrier culture has the advantages of both monolayer culture and suspension culture, and has the advantages of fast cell proliferation and wide adaptability. In the field of vaccine production, virus production or some immune cell culture, microcarrier culture can be widely used in the culture of some adherent cells, without the need for culture and acclimatization, maintaining the adherent characteristics of cells, and can make full use of cell culture medium, quickly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/02
CPCC12M47/04C12M33/14C12M27/02
Inventor 周进师传胤钱其军
Owner 上海乘黄纳米抗体科技有限公司
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