Method for improving enzymatic saccharification effect by xylose residue pretreatment

A technology of pretreatment and xylose residue, which is applied in climate change adaptation, fertilizer made from biological waste, agriculture, etc., can solve the problem of low hydrolysis efficiency of cellulase, and improve hydrolysis yield and reducing sugar yield, The effect of reducing discharge and reducing the discharge of waste liquid

Pending Publication Date: 2020-01-31
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Purpose of the invention: Aiming at the problems existing in the prior art, the present invention provides a method for improving the effect of enzymatic hydrolysis and saccharification by pretreatment of xylose residues. The clean and efficient pretreatment method of xylose residues of the present invention can improve the low efficiency of cellulase hydrolysis , so as to reduce the amount of cellulase and improve the conversion efficiency of reducing sugar

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  • Method for improving enzymatic saccharification effect by xylose residue pretreatment
  • Method for improving enzymatic saccharification effect by xylose residue pretreatment
  • Method for improving enzymatic saccharification effect by xylose residue pretreatment

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Embodiment 1

[0037] Weigh 30.0±0.1g of xylose residue, add 300.0±0.1g of DES (choline chloride: lactic acid (molar ratio 1:2)) for pretreatment at 90°C for 1 hour, and perform solid-liquid separation after the reaction. Then take the solid and add 300.0±0.1mL KOH solution with a mass fraction of 7%, pretreat at 70°C for 90min, and separate the solid from the liquid after the reaction; wash the solid part with tap water until it is neutral, and perform enzymatic hydrolysis Saccharification experiment. Enzymatic hydrolysis reaction volume is 20mL, add 3g of pretreated xylose residue (in dry basis) with 15wt.% solid loading, add 0.05mol / L citric acid buffer solution to adjust pH to 4.8, add 0.2mL of Sodium azide solution (0.02 g / mL), and then cellulase was added, the enzyme dosage was 5 FPU / g-substrate, and the reaction was carried out at 50° C. at 120 rpm for 72 h. Then HPLC was used to measure the glucose concentration in the enzymolysis solution. The results showed that the glucose conce...

Embodiment 2

[0039] Weigh 30.0±0.1g of xylose residue, add 300.0±0.1g of DES (choline chloride: lactic acid (1:2)) for pretreatment at 90°C for 1 hour, perform solid-liquid separation after the reaction, and then take Add 300.0±0.1mL of 10% KOH solution to the solid, pretreat at 70°C for 90min, and perform solid-liquid separation after the reaction. The solid part was washed to neutral with tap water, and the enzymatic hydrolysis and saccharification experiment was carried out. Enzymatic hydrolysis reaction volume is 20mL, add 3g of pretreated xylose residue (in dry basis) with 15wt.% solid loading, add 0.05mol / L citric acid buffer solution to adjust pH to 4.8, add 0.2mL of Sodium azide solution (0.02 g / mL), and then cellulase was added, the enzyme dosage was 5 FPU / g-substrate, and the reaction was carried out at 50° C. at 120 rpm for 72 h. Then HPLC was used to measure the glucose concentration in the enzymolysis solution. The results showed that the glucose concentration in the enzymol...

Embodiment 3

[0041] Weigh 30.0±0.1g of xylose residue, add 300.0±0.1g of DES (choline chloride: lactic acid (1:2)) for pretreatment at 90°C for 1 hour, perform solid-liquid separation after the reaction, and then take Add 300.0±0.1mL of KOH solution with a mass fraction of 13% to the solid, pretreat at 70°C for 90min, and perform solid-liquid separation after the reaction. The solid part was washed to neutral with tap water, and the enzymatic hydrolysis and saccharification experiment was carried out. Enzymatic hydrolysis reaction volume is 20mL, add 3g of pretreated xylose residue (in dry basis) with 15wt.% solid loading, add 0.05mol / L citric acid buffer solution to adjust pH to 4.8, add 0.2mL of Sodium azide solution (0.02 g / mL), and then cellulase was added, the enzyme dosage was 5 FPU / g-substrate, and the reaction was carried out at 50° C. at 120 rpm for 72 h. Then HPLC was used to measure the glucose concentration in the enzymolysis solution. The results showed that the glucose conc...

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Abstract

The invention discloses a method for improving an enzymatic saccharification effect by xylose residue pretreatment. The method comprises the following steps: weighing xylose residues, adding deep eutectic solvent (DES) to carry out pretreatment, and carrying out solid-liquid separation on the pretreated xylose residues after the reaction is finished; adding KOH solution into the separated solid for pretreatment, carrying out solid-liquid separation after the reaction is finished, and separately collecting solid and liquid parts; and washing the solid part to be neutral, thereby obtaining pretreated xylose residues, carrying out enzymatic saccharification, and determining the glucose concentration in the enzymatic hydrolysate. According to the invention, the method for clean and highly-efficient pretreatment of the xylose residues can solve a problem of low cellulose hydrolysis efficiency, thereby reducing the use level of the cellulose and improving the conversion efficiency of reducing sugar, thus providing a certain theoretical basis for the comprehensive utilization of the xylose residues.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for improving the effect of enzymatic hydrolysis and saccharification by pretreatment of xylose residue. Background technique [0002] Energy is the material basis for the sustainable development of the human economy and society. With the rapid development of the economy and the continuous improvement of people's living standards, the contradiction between the increasing energy demand and the increasingly depleted non-renewable resources has gradually become prominent. At the same time, problems such as ecological damage and environmental pollution caused by the development and utilization of fossil energy have gradually emerged. The massive emission of greenhouse gases has had a huge impact on the global climate. The frequent occurrence of various natural disasters has made ecological security and The sustainable development of human society has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C05F5/00
CPCC12P19/14C12P19/02C05F5/006C12P2201/00Y02A40/20
Inventor 王朝宇迟雪文毕艳红卢艺惠赵祥杰李明依
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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