Method for determining fatty acid in rice by using gas chromatography-mass spectrometry

Abstract

The invention discloses a method for determining fatty acid in rice by using the gas chromatography-mass spectrometry. The method comprises the following steps: (1) preparation of a sample to be determined: pulverizing a rice sample, sequentially adding n-hexane, an acetyl chloride-methanol solution and butylated hydroxytoluene, mixing, and heating to perform fatty acid extraction and methyl esterification treatment; cooling to room temperature, adding a sodium carbonate solution, uniformly mixing, centrifuging, taking supernatant, and filtering to obtain a sample to be detected; and (2) determination of fatty acid components: performing quantitative or qualitative determination on 37 kinds of fatty acids of the sample obtained to be detected in the step (1) by adopting a gas chromatograph-mass spectrometer. According to the present invention, through the common cooperation of the extraction method and the determination method, the accurate qualitative and quantitative determination of37 kinds of fatty acids in rice is achieved, the difficult problem that the fatty acid components in rice cannot be accurately determined in the prior art is solved, and the determination of a variety of fatty acids can be achieved on the basis of the assurance of the determination accuracy of the fatty acid components.

Description

technical field [0001] The invention relates to the technical field of fatty acid determination, in particular to a method for determining fatty acid in rice by gas chromatography-mass spectrometry. Background technique [0002] Fat is the main source of human energy intake, and more importantly, it can provide the essential fatty acids needed by the human body. Plant fatty acid is an important component of plant nutrition. Its composition and content ratio determine the quality, nutritional value and biological effect of vegetable oil. Plant fatty acid is mainly divided into saturated fatty acid and unsaturated fatty acid. The determination of its content is very important for the analysis of plant nutrition. The quality of the value plays a crucial role. Therefore, developing a method that can accurately detect plant fatty acids will have a profound impact on understanding the composition of plant fatty acid components, improving the oil content in plants, improving human...

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Problems solved by technology

[0004] Although with the development of high-sensitivity mass spectrometry technology, gas chromatography-mass spectrometry technology has also appeared to measure fatty acids at present, but the current gas chromatography-mass spectrometry technology can only realize the determination of relatively few types of fatty acids. The determination of many and complex fatty acid components has poor accuracy. Due to the complex types of fatty acids in rice and their uneven content, there are few reports on the determination of fatty acid components in rice, let alone how to use gas chromatography-mass spectrometry. Relevant research and reports on the realization of accurate determination of fatty acid components in rice

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