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Preparation method of grape seed extract with high antimicrobial activity

A grape seed and extract technology is applied in skin care preparations, medical preparations with inactive ingredients, medical preparations containing active ingredients, etc. Effect

Active Publication Date: 2020-02-07
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to overcome the fact that grape seeds are rich in polyphenols in the prior art, have certain bacteriostasis, and can be widely used in food, medicine, cosmetics and other fields for bacteriostasis, but their bacteriostasis performance needs to be improved , the antibacterial effect needs to be compounded with other components to meet the use requirements. A preparation method of grape seed extract with high antibacterial property is provided, and the grape seed extract is modified by low-temperature plasma technology, which effectively improves the Its antibacterial activity

Method used

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  • Preparation method of grape seed extract with high antimicrobial activity

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Grape seeds are pulverized, and 80 mesh sieves are obtained to obtain grape seed powder;

[0025] (2) Adding ethanol with a volume fraction of 50% to the grape seed powder, the solid-liquid ratio is 1:8, 40° C., ultrasonic extraction at 40 Hz for 45 min to obtain the grape seed extract;

[0026] (3) vacuum-drying the grape seed extract at 70°C to obtain the grape seed extract;

[0027] (4) Treat the grape seed extract with a low-temperature plasma atmosphere to obtain a highly bacteriostatic grape seed extract. The processing power is 700W, and the processing time is 13min. The working gas that produces a low-temperature plasma atmosphere is O with a volume ratio of 2:1. 2 and N 2 .

Embodiment 2

[0029] (1) Grape seeds are pulverized, and the grape seed powder is obtained through a 60-mesh sieve;

[0030] (2) Adding ethanol with a volume fraction of 40% to the grape seed powder, the solid-liquid ratio is 1:7, 35° C., ultrasonic extraction at 45 Hz for 50 min to obtain the grape seed extract;

[0031] (3) vacuum-drying the grape seed extract at 60°C to obtain the grape seed extract;

[0032] (4) Treat the grape seed extract with a low-temperature plasma atmosphere to obtain a highly bacteriostatic grape seed extract. The processing power is 600W, and the processing time is 15min. The working gas that produces a low-temperature plasma atmosphere is O with a volume ratio of 1:1. 2 and N 2 .

Embodiment 3

[0034] (1) Grape seeds are pulverized, and the grape seed powder is obtained through a 100-mesh sieve;

[0035] (2) Adding ethanol with a volume fraction of 60% to the grape seed powder, the solid-liquid ratio is 1:9, 55° C., ultrasonic extraction at 35 Hz for 40 min to obtain the grape seed extract;

[0036] (3) vacuum-drying the grape seed extract at 80°C to obtain the grape seed extract;

[0037] (4) Treat grape seed extract with low-temperature plasma atmosphere to obtain highly bacteriostatic grape seed extract, processing power 800W, processing time 10min, the working gas that produces low-temperature plasma atmosphere is the O that volume ratio is 3:1 2 and N 2 .

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Abstract

The invention relates to the technical field of plant extract modification, and discloses a preparation method of a grape seed extract with high antimicrobial activity. The preparation method comprises the steps: grape seeds are screened and sieved to obtain grape seed powder; ethyl alcohol is added into the grape seed powder, and a grape seed extracting solution is obtained through ultrasonic extraction; after the grape seed extracting solution is subjected to vacuum drying, a grape seed extract is obtained; and the grape seed extract is treated through a low-temperature plasma atmosphere, and the grape seed extract with the high antimicrobial activity is obtained. High-energy particles and active components of low-temperature plasmas act on the surface of the grape seed extract so that the surfaces of components of polyphenols in the grape seed extract can be modified, and thus the antimicrobial activity of the grape seed extract is effectively improved.

Description

technical field [0001] The invention relates to the technical field of plant extract modification, in particular to a preparation method of grape seed extract with high antibacterial properties. Background technique [0002] Grapes are one of the most commonly cultivated fruits in the world. China produces about 1.4 million tons of grapes annually, of which about 80% are used for winemaking. Grape seeds are a by-product of the wine-making industry. During the wine-making process, 3% of the total grape seeds are produced. [0003] Grape seeds are rich in polyphenols, which have certain antibacterial and antioxidant properties. Therefore, grape seed extracts rich in polyphenols have been widely used in food, medicine, cosmetics and other fields for antibacterial. For example, "A method for antibacterial and fresh-keeping of fresh pork by compound plant polyphenols" disclosed in the Chinese patent literature, its announcement number CN109042830A, the invention uses tea polyphe...

Claims

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Application Information

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IPC IPC(8): A23L3/3472A61K8/9789A61Q19/00A61K47/46
CPCA23L3/3472A61K8/9789A61Q19/00A61K47/46A23V2002/00A61K2800/524A61K2800/82A23V2200/10A23V2250/21166
Inventor 缪文华汪舟娜
Owner ZHEJIANG OCEAN UNIV
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