Preparation method of probiotics compound freeze-dried powder
A technology of probiotics and freeze-dried powder, which is applied in the preservation of microorganisms, the function of food ingredients, food science, etc., can solve the problems of low number of viable bacteria in freeze-dried powder, unstable effect, and easy death of probiotics
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Embodiment 1
[0021] The present embodiment provides a preparation method of probiotic compound freeze-dried powder, comprising the following steps:
[0022] Step 1. Preparation of fruit and vegetable powder: Select 4 parts of sweet potato, 5 parts of corn, 3 parts of yam, 5 parts of apple, 8 parts of strawberry and 4 parts of kiwi fruit that are free from pests and diseases, without mechanical damage, and of the same size, and then cleaned, cored and sliced in sequence , placing the sliced fruits and vegetables in a dehydration device for drying and dehydration for 30 minutes, placing the dried and dehydrated fruits and vegetables in a grinder for grinding, and passing through a 200-400 mesh sieve to obtain fruit and vegetable powder;
[0023] Step 2. Probiotic culture: Weigh a certain amount of grape bran, peptone, meat extract and yeast extract, mix and add a small amount of hot water to completely dissolve to prepare MRS medium, and then add lactic acid bacteria medium booster to the...
Embodiment 2
[0027] The present embodiment provides a preparation method of probiotic compound freeze-dried powder, comprising the following steps:
[0028] Step 1. Preparation of fruit and vegetable powder: Select 5 parts of sweet potato, 8.5 parts of corn, 6 parts of yam, 7 parts of apple, 11 parts of strawberry and 6 parts of kiwi fruit without pests and diseases, without mechanical damage, and of the same size, and then wash, core and slice in turn. , placing the sliced fruits and vegetables in a dehydration device for drying and dehydration for 45 minutes, placing the dried and dehydrated fruits and vegetables in a grinder for grinding, and passing through a 200-400 mesh sieve to obtain fruit and vegetable powder;
[0029] Step 2. Probiotic culture: Weigh a certain amount of grape bran, peptone, meat extract and yeast extract, mix and add a small amount of hot water to completely dissolve to prepare MRS medium, and then add lactic acid bacteria medium booster to the medium Stir and ...
Embodiment 3
[0033] The present embodiment provides a preparation method of probiotic compound freeze-dried powder, comprising the following steps:
[0034] Step 1. Prepare fruit and vegetable powder: select 6 parts of sweet potato, 12 parts of corn, 9 parts of yam, 9 parts of apple, 14 parts of strawberry and 8 parts of kiwi fruit without pests and diseases, without mechanical damage, and of the same size, and then wash, core and slice them in turn. , placing the sliced fruits and vegetables in a dehydration device for drying and dehydration for 60 minutes, placing the dried and dehydrated fruits and vegetables in a grinder for grinding, passing through a 200-400 mesh sieve to obtain fruit and vegetable powder;
[0035] Step 2. Probiotic culture: Weigh a certain amount of grape bran, peptone, meat extract and yeast extract, mix and add a small amount of hot water to completely dissolve to prepare MRS medium, and then add lactic acid bacteria medium booster to the medium Stir and dissolv...
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