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A method for the determination of tobacco sweeteners by high performance liquid chromatography

A high-performance liquid chromatography and sweetener technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of low detection efficiency and sensitivity of cigarette sweeteners, and achieve high sensitivity, short analysis time, and simple operation. Effect

Active Publication Date: 2022-04-19
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for the determination of tobacco sweeteners by high performance liquid chromatography, to solve the problem of low detection efficiency and sensitivity of tobacco sweeteners in the prior art

Method used

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  • A method for the determination of tobacco sweeteners by high performance liquid chromatography
  • A method for the determination of tobacco sweeteners by high performance liquid chromatography
  • A method for the determination of tobacco sweeteners by high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0053] 1. Instruments and reagents

[0054] The 5 sweeteners and methanol are all chromatographic grade reagents; they meet the requirements of first-grade water in GB / T 6682.

[0055] High performance liquid chromatography (Agilent 1260Infinity LC, American Agilent Company); diode array detector (Agilent 1260Infinity DAD, American Agilent Company); Hukang centrifuge (TG16-WS, Hunan Hukang Centrifuge Co., Ltd.); MS303S electronic balance (Sensitivity 0.0001g, Mettler Toledo, Switzerland); full-temperature oscillator (HZQ-F160, Okura Experimental Equipment Factory).

[0056] 2. Extraction of samples

[0057] Weigh an appropriate amount of tipping paper sample, after pretreatment, add an appropriate amount of ultrapure water for mechanical vibration extraction, let it stand still, take an appropriate amount of extract to pass through a 0.22 μm organic filter membrane, and perform HPLC-DAD analysis.

[0058] 3. Prepare the standard working solution; see the preparation of the sta...

example 2

[0065] 1. Instruments and reagents

[0066] The 5 sweeteners and methanol are all chromatographic grade reagents; they meet the requirements of first-grade water in GB / T 6682.

[0067] High-performance liquid chromatography (Agilent 1260Infinity LC, Agilent Corporation of the United States); diode array detector (Agilent 1260Infinity DAD, Agilent Corporation of the United States); ultrasonic extraction apparatus (SB-3200DT, Ningbo Xinzhi Biotechnology Co., Ltd.).

[0068] 2. Extraction of gum-based cigarette samples

[0069] Weigh an appropriate amount of gum-based tobacco sample, after pretreatment, add an appropriate amount of extract solution for ultrasonic vibration extraction, absorb an appropriate amount of extract solution and filter it through a 0.22 μm water phase filter membrane into a chromatographic bottle, and use it as a sample solution for HPLC-DAD analysis.

[0070] 3. Prepare standard working solution

[0071] 4. HPLC-DAD determination and analysis.

[0072...

example 3

[0077] 1. Instruments and reagents

[0078] The 5 sweeteners and methanol are all chromatographic grade reagents; they meet the requirements of first-grade water in GB / T 6682.

[0079] High-performance liquid chromatography (Agilent 1260Infinity LC, Agilent Corporation of the United States); diode array detector (Agilent 1260Infinity DAD, Agilent Corporation of the United States); ultrasonic extraction apparatus (SB-3200DT, Ningbo Xinzhi Biotechnology Co., Ltd.).

[0080] 2. Extraction of bagged snus samples

[0081] Weigh an appropriate amount of snuff sample, after pretreatment, add an appropriate amount of extract solution for ultrasonic oscillation, draw an appropriate amount of extract solution and filter it through a 0.22 μm water phase filter membrane into a chromatographic bottle, and use it as a sample solution for HPLC-DAD analysis.

[0082] 3. Prepare standard working solution

[0083] 4. HPLC-DAD determination and analysis.

[0084] 5. The content of each sweete...

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Abstract

The invention relates to a method for determining tobacco sweeteners by high performance liquid chromatography. The technical scheme adopts a pretreatment method of solvent extraction, and establishes HPLC-DAD to simultaneously measure cigarette tipping paper and oral smokeless tobacco products. , tobacco flavor, tobacco popping beads, the detection and analysis methods of five sweeteners in electronic cigarette liquid, including acesulfame-K, aspartame, neotame, alitame and saccharin sodium, and use the established method to analyze some Samples were tested. The method is simple in operation, short in analysis time and high in sensitivity, and provides a technical means for detecting sweeteners in smoking materials and tobacco products.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical inspection of food additive residues in tobacco materials and tobacco products, and mainly relates to tipping paper for cigarettes, oral smokeless tobacco products, flavors for tobacco, popping beads for cigarettes, and 5 A method for determining sweeteners, specifically a method for extracting sweeteners by means of solvent extraction and directly determining by high performance liquid chromatography-diode array method. Background technique [0002] Sweeteners are usually used in food to achieve the purpose of improving flavor and increasing sweetness, and can be divided into natural sweeteners and artificial sweeteners according to the source. Commonly used natural sweeteners include steviol glycoside, mogroside V, etc. Commonly used synthetic sweeteners mainly include acesulfame potassium, sodium saccharin, alitame, cyclamate, sucralose, etc., and their sweetness is generally sucr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/88G01N30/06G01N2030/062G01N2030/8809
Inventor 李文伟赵海娟王晖张文洁张峻松李瑞丽贾林涛张瑞涛靳毅索楠
Owner CHINA TOBACCO HENAN IND