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A kind of polypeptide synthetic method

A technology for peptide synthesis and synthesis method, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of pollution, reverse flow of waste liquid, entering nitrogen pipes, etc. Effect

Active Publication Date: 2021-08-06
广州市雅彩盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, in order to solve the problems that the waste liquid in the polypeptide synthesis equipment flows backwards, enters the nitrogen pipeline, causes pollution, and the solid phase of the polypeptide adheres to the polypeptide synthesis equipment, the present invention provides a polypeptide synthesis method

Method used

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  • A kind of polypeptide synthetic method
  • A kind of polypeptide synthetic method
  • A kind of polypeptide synthetic method

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Experimental program
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Effect test

preparation example Construction

[0029] like Figure 1-5 As shown, a polypeptide synthesis method according to the present invention has a synthesis method comprising the steps of:

[0030] S1: First, dry the alfalfa, then break the wall, maintain the granular particle size between 120-150, followed by filtration, and add cellulase, and use ultrasound extraction equipment, The pulverization of the microporous filter bag body is subjected to continuous extraction;

[0031] S2: Transfer the ultrasound extraction product in S1 into the fermentation apparatus, add proteolytic enzyme to the fermentation apparatus, and control the fermentation temperature between 50-70 ° C, and the decomposition time is controlled within 3-4 hours;

[0032] S3: After the decomposition is completed, the reaction to decompose fermentation is also transferred to the centrifugal apparatus, and the decomposition liquid in the centrifugal separation apparatus is separated by a layered biofilm to obtain an amino acid product;

[0033] S4: The ...

Embodiment approach

[0035] As an embodiment of the present invention, the control unit 3 includes a control valve 31, a controller 32, a controller wheel 33, a control spool outlet 34, and a control spool inlet 35; the control spool 32 center position The control valve 31 front end controls the hand wheel 33, and the inside of the control spool 32 is provided with a cavity 321, and the cavity 321 is provided with a seal ball 322, and the sealing ball 322 is connected to the cavity 321 inner wall connection, control. The outer wall of the spool 32 has a control spool outlet 34, and the control spool outlet 34 penetrates to the inside cavity 321 of the control spool 32, and the external wall of the control spool 32 has a control spool inlet 35, and the control spool inlet 35 penetrates into the Control the inner cavity 321 of the spool 32, and control the direction of the spool inlet 35 and the control spool outlet 34 is perpendicular; through the control unit 3, the flow of nitrogen gas flow into the ...

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Abstract

The invention belongs to the technical field of polypeptide synthesis, and specifically relates to a polypeptide synthesis method. The synthesis equipment used in the synthesis method includes a bracket and a synthesis tank; The output shaft is connected to one end of the rotating shaft provided in the synthetic tank, and the other end of the rotating shaft is connected to multiple stirring plates; a heating chamber is provided between the outer wall of the synthetic tank and the inner wall of the synthetic tank; the inner wall of the synthetic tank is provided with a No. A number of air outlets are opened, and the air outlets communicate with the No. 1 pipeline on the lower end of the No. 1 plate through the No. 1 plate, and the control unit is used to control the passage of nitrogen into the No. 1 pipeline and the waste liquid in the synthesis tank to flow out from the No. 1 pipeline , the left end of the control unit is provided with the No. 2 pipe for waste liquid outflow, and the right end of the control unit is provided with the No. 3 pipe for nitrogen gas inlet; through the forward and reverse motor, the synthesis tank, the No. 1 pipe, the control unit, the No. 2 pipe and the No. 3 pipe , to realize the synthesis of peptides in the synthesis tank.

Description

Technical field [0001] The present invention belongs to the field of polypeptide synthesis, and is specifically a polypeptide synthesis method. Background technique [0002] The polypeptide is a compound formed by the alpha-amino acid to be formed together with a peptide bond, and is an intermediate product of protein hydrolysis. Compounds formed by dehydration of two amino acid molecules are called anticipeptides, and there are tri peptides, tetrapeptides, pentin peptides, and the like. The compound typically consisted of 10-100 amino acid molecules is called a polypeptide. [0003] The polypeptide synthesis is a solid phase synthesis sequence generally synthesize from the N-terminal (amino end) to the C-terminal (carboxy). The past polypeptide synthesis is called a liquid phase synthesis method in solution. Since the development of Merrifield in 1963, the solid phase polypeptide synthesis process has been continuously improved and improved. Today, the solid phase method has bec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/04C07K1/06F17D1/00F17D3/01
CPCC07K1/04C07K1/06F17D1/005F17D3/01
Inventor 沈红超孔伟伟苏生鹏
Owner 广州市雅彩盛生物科技有限公司