Method for preparing oil rich in saturated and unsaturated fatty acids by using Penicillium ascidianum asc2-4
A technology of unsaturated fatty acid and sea squirt orange, applied in the field of biological extraction, can solve the problem of high content
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 2
[0115] A method for preparing oil rich in saturated and unsaturated fatty acids by using Penicillium citrinum Asc2-4, comprising the following steps:
[0116] S1. Inoculate Penicillium citrinum Asc2-4 into the seed medium, and culture it in a shaker at 26°C and 200rpm for 3 days; the composition of the seed medium is: potato extract powder 8g / L, glucose 20g / L, sea essence salt 15g / L, ammonium sulfate 10g / L;
[0117] S2. inoculate the cultivated seeds in the fermentation medium, and ferment for 5 days in a shaker at 26° C. to obtain the Penicillium citrinum mycelium under the condition that the liquid loading is 40% of the volume of the culture vessel and the rotating speed is 220 rpm. The composition of the fermentation medium is: glucose 100g / L, potato 200g / L, sodium citrate 0.1g / L, sea essence salt 15g / L, peptone 0.5g / L, yeast extract 0.5g / L, KH 2 PO 4 4g / L, MgSO 4 150mg / L, FeCl 3 2.0μg / L; the mass ratio of yeast extract to peptone is 1:1;
[0118] S3. Extract oil by...
Embodiment 3
[0120] A method for preparing oil rich in saturated and unsaturated fatty acids by using Penicillium citrinum Asc2-4, comprising the following steps:
[0121] S1. Inoculate Penicillium citrinum Asc2-4 into the seed medium, and culture it in a shaker at 28°C and 180rpm for 2 days; the composition of the seed medium is: potato extract powder 6g / L, glucose 18g / L, sea essence salt 13g / L, ammonium sulfate 8g / L;
[0122] S2. inoculate the cultivated seeds in the fermentation medium, and ferment for 5 days in a shaker at 28° C. to obtain the Penicillium citrinum mycelium under the condition that the liquid loading is 40% of the volume of the culture vessel and the rotating speed is 180 rpm. The composition of the fermentation medium is: glucose 50g / L, potato 150g / L, sodium citrate 0.1g / L, sea essence 10g / L, peptone 0.1g / L, yeast extract 0.1g / L, KH 2 PO 4 2g / L, MgSO 4 50mg / L, FeCl 3 2.0μg / L; the mass ratio of yeast extract to peptone is 1:1;
[0123] S3. Extract oil by acid he...
Embodiment 4
[0125] A method for preparing oil rich in saturated and unsaturated fatty acids by using Penicillium citrinum Asc2-4, comprising the following steps:
[0126] S1. Inoculate Penicillium citrinum Asc2-4 into the seed medium, and culture it in a shaker at 28°C and 180rpm for 2 days; the composition of the seed medium is: potato extract powder 6g / L, glucose 18g / L, sea essence salt 13g / L, ammonium sulfate 8g / L;
[0127] S2. Inoculate the cultivated seeds in the fermentation medium, and ferment for 3 days in a shaker at 26° C. to obtain the Penicillium citrinum mycelium under the condition that the liquid loading is 80% of the volume of the culture vessel and the rotating speed is 180 rpm. The composition of the fermentation medium is: glucose 150g / L, potato 200g / L, sodium citrate 0.5g / L, sea essence 20g / L, peptone 1.5g / L, yeast extract 1.5g / L, KH 2 PO 4 6g / L, MgSO 4 150mg / L, FeCl 3 6μg / L; the mass ratio of yeast extract to peptone is 1:1;
[0128] S3. Extract oil by acid he...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More