Method for determining content of volatile N-nitrosamine compound in plasma by gas chromatography-mass spectrometry
A technique for gas chromatography and mass spectrometry, applied in the field of determining the content of volatile N-nitrosamine compounds in plasma
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[0025] The invention is a method for determining the content of volatile N-nitrosamine compounds in blood plasma by gas chromatography-mass spectrometry, comprising the following steps:
[0026] (1) Take the blood sample and centrifuge to separate the plasma; take the plasma, add dichloromethane and NDPA-D14 (N-nitrosodi-n-propyl-d 14 -amine) internal standard standard solution, vortex in a vortex mixer, mix well and then ultrasonically extract; centrifuge again after completion; take the lower layer of liquid and inject. Preferably, the blood sample is centrifuged at 3000r / min and 4°C for 2.5-3.5min to separate the plasma. Preferably, calculated by volume fraction, take 1 part of plasma, add 0.45-0.55 part (most preferably 0.5 part) of methylene chloride and 0.045-0.055 part (most preferably 0.05 part) concentration of 500ug / L NDPA-D14 internal standard standard The solution, that is, the volume ratio of plasma, dichloromethane and NDPA-D14 internal standard solution is 1:0....
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