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Tissue-culture rapid propagation method for Cremastra appendiculata seedlings

A technology of tissue culture rapid propagation and seedlings, applied in the field of plant breeding, can solve the problems of low induction rate, unsatisfactory large-scale planting of Rhododendron orchids, and low survival rate of seedlings, so as to achieve good growth, solve plant resources and seedling resources Effects of shortage, differentiation rate, and increased survival

Inactive Publication Date: 2020-02-14
遵义市龙驰生物科技有限公司
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AI Technical Summary

Problems solved by technology

The induction rate in the process of this method is too low, and the survival rate of the obtained seedlings is not high, which does not meet the needs of large-scale planting of Rhododendron orchids. It is urgent to find a method that can improve the survival rate and can be planted on a large scale.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Seed treatment: Take mature uncracked fruit clips, wipe the surface with 75% alcohol, soak in 2% NaClO for 10 minutes, then wash 5 times with sterile water, dry the surface water in the shade, cut open the sterilized fruit clips, take out seeds and inoculate In the seed germination medium, during the process of taking seeds, take 1g of seeds with an analytical balance to obtain the number of seeds, and then weigh about 1000 seeds according to the proportion.

[0020] Seed germination: Sow the treated seeds in 1 / 4MS+6-BA0.1mg / L+NAA0.05mg / L+peptone 5g / L+potato 30g / L+banana 60g / L+agar 5g / L+sugar 30g / L, the pH is 6.0 seed germination medium, cultured for 5 months, in which the first month was in complete darkness, and after 1 month, it was cultured in an environment with a light of 1200 Lx and 12h / d.

[0021] Round bulb induction: transfer germinated seeds into improved MS+6-BA3.0mg / L+NAA1.0mg / L+vitamin C5g / L+peptone 10g / L+potato 30g / L+banana 60g / L+agar 2g / L+sugar 60g / L+a...

Embodiment 2

[0026] On the basis of Example 1, carry out plant domestication: ① preparation of differentiated seedlings: cut off the differentiated seedlings, soak in 500 times of carbendazim + 1000 times of eutrophicin for 10-15 minutes for later use; ② seedbed preparation: In a simple greenhouse, a small arched shed with a height of 1m and a width of 1m is made of bamboo chips, and a special substrate for seedling cultivation with a thickness of 10-15cm is laid in the arched shed to make a domesticated seedbed. The substrate was disinfected; ③Transplanting: transplant the differentiated seedlings into the domesticated seedbed with a row-to-plant spacing of 3cm, and cover the bamboo shed with a plastic film after transplanting; ④Management: keep the humidity in the seedbed at 80 -90%, the temperature is 26-28°C, and the self-made nutrient solution is sprayed every 10 days. After 3 months, high-quality seedlings can be transplanted into the field. The domestication process period is relativ...

Embodiment 3

[0028] In the round corm induction process of Example 1, the improved MS is that the KNO in the MS medium 3 , NH 4 NO 3 、KH 2 PO 4 , VB1, and VB6 were adjusted to 1500mg / L, 1300mg / L, 200mg / L, 1mg / L, and 1mg / L, respectively, and the remaining components and concentrations were unchanged.

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Abstract

The invention discloses a tissue-culture rapid propagation method for Cremastra appendiculata seedlings and belongs to the technical field of plant propagation. The method includes: sowing mature seeds into germination culture media, culturing for 5 months, transferring the germinate seeds into protocorm-inducing culture media to perform culture for 3 months to obtain a large number of protocorms,culturing the protocorms in differentiation culture media for 2 months to obtain plants with roots through differentiation, and taking out the plants to perform acclimation for 3 months to obtain high-quality seedlings which can be transplanted in a field. The method has the advantages that Cremastra appendiculata propagation rate is improved, reliable technical support is provided for the large-scale industrial production of the Cremastra appendiculata seedlings, a new approach is provided for the large-scale Cremastra appendiculata propagation, and the current problem of Cremastra appendiculata plant resource and Cremastra appendiculata seedling resource shortage is solved effectively.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for tissue culture and rapid propagation of Du Juanlan seedlings. Background technique [0002] Rhododendron orchid is a plant in the family Orchidaceae. It is the basic plant of the Chinese medicinal material Arrowhead mushroom. The pseudobulb is used as medicine. It has the effects of clearing away heat and detoxification, moistening the lungs and relieving cough, promoting blood circulation and relieving pain, reducing swelling and dispelling stagnation, and is an important and scarce Chinese herbal medicine. Rhododendron orchid likes a cool and humid environment and has strict requirements on the ecological environment. At present, it mainly relies on ramets for reproduction, and its reproduction coefficient is extremely low. [0003] With the increasing demand for Chinese herbal medicine Arrowhead in the market of Chinese medicinal materials, people have car...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 刘红美龚晓林梁大勇石建龙
Owner 遵义市龙驰生物科技有限公司
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