Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of dna nano controlled release drug delivery molecule that can be used for breast cancer and other tumors and its application

A drug delivery and molecular technology, applied in the direction of antineoplastic drugs, drug combinations, pharmaceutical formulations, etc., can solve the problems of unseen patents, etc., and achieve the effects of good biocompatibility, avoiding damage, and controllable release

Active Publication Date: 2022-04-05
NANJING MEDICAL UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant patent on the use of telomerase as a trigger for DNA biomacromolecular materials as nucleic acid drug carriers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of dna nano controlled release drug delivery molecule that can be used for breast cancer and other tumors and its application
  • A kind of dna nano controlled release drug delivery molecule that can be used for breast cancer and other tumors and its application
  • A kind of dna nano controlled release drug delivery molecule that can be used for breast cancer and other tumors and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] figure 1 Schematic diagram of the construction of the DNA nano-controlled release siRNA delivery system. Take 1 μL single-stranded DNA (10 μM) each for Prism-1, Prism-2, Prism-3, Prism-4, Prism-5, Prism-6, and 1 μL single-stranded siRNA (10 μM) with P-7 sequences linked at both ends , and 1 μL of another complementary siRNA single strand (10 μM) were added to 12 μL of PBS (8 mM Na 2 HPO 4 , 0.137mM NaCl, 2mM NaH 2 PO 4 , pH7.2-7.4) solution. When fluorescent observation is required for subsequent experiments, the corresponding single strands with fluorescent modifications can be used. The mixed solution was placed in a PCR machine, and the temperature was slowly and uniformly cooled from 95°C to 4°C within 6.5 hours, that is, the temperature was lowered by 1°C in about 4 minutes and 18s, so as to realize the self-assembly of nucleic acids. A single strand of DNA can self-assemble into a three-dimensional DNA hexahedron through complementary base pairing. image 3...

Embodiment 2

[0038] figure 1 Schematic diagram of the construction of the DNA nano-controlled release siRNA delivery system. Each take 1 μL of single-stranded DNA (10 μM) Prism-1-PEG, Prism-2-PEG, Prism-3-PEG, Prism-4-PEG, Prism-5-PEG, Prism-6-PEG, and, and 1 μL of two The siRNA single strand (10 μM) with the P-7 sequence connected at the end, and 1 μL of another complementary siRNA single strand (10 μM) were added to 12 μL PBS (8 mM Na 2 HPO 4 , 0.137mM NaCl, 2mM NaH 2 PO 4 , pH7.2-7.4) solution. When fluorescent observation is required for subsequent experiments, the corresponding single strands with fluorescent modifications can be used. The mixed solution was placed in a PCR machine, and the temperature was slowly and uniformly cooled from 95°C to 4°C within 6.5 hours, that is, the temperature was lowered by 1°C in about 4 minutes and 18s, so as to realize the self-assembly of nucleic acids. A single strand of DNA can self-assemble into a three-dimensional DNA hexahedron through ...

Embodiment 3

[0043] figure 1 Schematic diagram of the construction of the DNA nano-controlled release siRNA delivery system. Take 1 μL each of single-stranded DNA (10 μM) Prism-1-S, Prism-2-PEG, Prism-3-S, Prism-4-S, Prism-5-PEG, Prism-6-S, and 1 μL of both ends ligation siRNA single strand (10 μM) with P-7 sequence, and 1 μL of another complementary siRNA single strand (10 μM) were added to 12 μL PBS (8 mM Na 2 HPO 4 , 0.137mM NaCl, 2mM NaH 2 PO 4 , pH7.2-7.4) solution. When fluorescent observation is required for subsequent experiments, the corresponding single strands with fluorescent modifications can be used. The mixed solution was placed in a PCR machine, and the temperature was slowly and uniformly cooled from 95°C to 4°C within 6.5 hours, that is, the temperature was lowered by 1°C in about 4 minutes and 18s, so as to realize the self-assembly of nucleic acids. A single strand of DNA can self-assemble into a three-dimensional DNA hexahedron through complementary base pairing....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DNA nanometer controlled-release drug delivery molecule that can be used for breast cancer and other tumors and its application. The drug delivery molecule is a DNA single-strand combination that can self-assemble into a DNA hexahedral frame structure through complementary base pairing and two ends DNA hexahedrons loaded with nucleic acid drugs and having telomerase extension primers are obtained after the nucleic acid drugs connected with drug-loaded single strands are mixed and annealed. In the present invention, a DNA chain complementary to a DNA hexahedron frame structure sequence is connected to both ends of an RNA chain of the siRNA, thereby realizing the loading of the siRNA in the DNA hexahedron. At the same time, the present invention connects a telomerase extension primer to the DNA hexahedral frame structure, so that it can be recognized and extended by telomerase, which is generally highly expressed in tumor cells such as breast cancer, and is complementary to the siRNA binding position on the frame structure, so that the siRNA The controllable release of nucleic acid drugs in response to telomerase can be achieved by detaching from the DNA hexahedral framework due to the small free energy. The targeted drug-carrying molecule has very high specific recognition function, low cytotoxicity and good structural stability.

Description

technical field [0001] The invention belongs to the field of life medicine, and relates to a DNA nano-controlled drug delivery molecule for breast cancer and other tumors and its application. Background technique [0002] Whether in the world or in China, cancer has become the leading cause of population death, a killer that endangers human health and an important public health problem. siRNA has important application prospects in the clinical treatment of tumors, but due to its difficult to enter cells and easy to degrade, it needs to be delivered intracellularly with the help of carriers. [0003] The research on nano-drug carriers for siRNA delivery has matured, and the current mainstream siRNA drug carriers mainly include the following categories: natural or synthetic lipids (Chinese invention patent: a siRNA targeting HBx and its liposome preparation, Publication number: CN104031917B; Chinese invention patent: a siRNA-PLGA / CSO conjugate micelle and its preparation meth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/26A61K31/7105A61K48/00A61P35/00
CPCA61K9/5123A61K31/7105A61P35/00
Inventor 管晓翔陆妍
Owner NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com