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Chlorella fermentation extract and preparation method thereof

The technology of extract and chlorella is applied in the field of chlorella fermented extract and its preparation, which can solve the problems of lack of research and reports on the application in the field of cosmetics, and achieves the improvement of type I collagen synthesis ability and improvement of anti-aging. , the effect of improving the survival rate

Pending Publication Date: 2020-02-18
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the research on the application of chlorella is mainly concentrated in the fields of food, medicine, energy and aquaculture, while there are few researches and reports on the application of chlorella in the field of cosmetics.

Method used

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  • Chlorella fermentation extract and preparation method thereof
  • Chlorella fermentation extract and preparation method thereof
  • Chlorella fermentation extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Preparation of chlorella fermented extract

[0031] Adopt the following method to prepare the chlorella fermented extract:

[0032] (1) Seed preparation: inoculate the preserved Lactobacillus plantarum into the seed medium, and culture at 30°C for 24 hours to obtain a bacterial suspension;

[0033] The composition and content of the seed medium are: M17 medium, glucose 20g / l, sterilized at 115°C for 30min.

[0034] (2) Liquid fermentation culture: inoculate the bacterium suspension cultivated in step (1) in the fermentation medium with an inoculum size of 10% by volume, and cultivate it statically at 30°C for 36h, and the glucose content is 0, and the fermentation is completed to obtain fermentation broth;

[0035] The composition and content of the fermentation medium are: chlorella powder 10g / l, glucose 15g / l, the rest is distilled water, sterilized at 115°C for 30min.

[0036] (3) Adjust pH: Use 1mol / L NaOH solution to adjust the pH of the fermentati...

Embodiment 2

[0042] Embodiment 2: Preparation of chlorella fermented extract

[0043] Adopt the following method to prepare the chlorella fermented extract:

[0044] (1) Seed preparation: inoculate the preserved Lactobacillus plantarum into the seed medium, and culture it at 30°C for 28 hours to obtain a bacterial suspension;

[0045] The composition and content of the seed medium are: M17 medium, glucose 15g / l, sterilized at 115°C for 30min.

[0046] (2) Liquid fermentation culture: inoculate the bacterium suspension cultured in step (1) in the fermentation medium with an inoculum size of 15% by volume, and cultivate it statically at 30°C for 30h, the glucose content is 0, and the fermentation is finished to obtain fermentation broth;

[0047] The composition and content of the fermentation medium are: chlorella powder 10g / l, glucose 12g / l, the rest is distilled water, sterilized at 115°C for 30min.

[0048] (3) Adjust pH: Use 1mol / L NaOH solution to adjust the pH of the fermentation b...

Embodiment 3

[0050] Embodiment 3: Preparation of chlorella fermented extract

[0051] Adopt the following method to prepare the chlorella fermented extract:

[0052] (1) Seed preparation: inoculate the preserved Lactobacillus plantarum into the seed medium, and culture at 30°C for 20 hours to obtain a bacterial suspension;

[0053] The composition and content of the seed medium are: M17 medium, glucose 10g / l, sterilized at 115°C for 30min.

[0054] (2) Liquid fermentation culture: inoculate the bacterium suspension cultured in step (1) in the fermentation medium with an inoculum size of 20% by volume, and cultivate it statically at 30°C for 38h, the glucose content is 0, and the fermentation is finished to obtain fermentation broth;

[0055] The composition and content of the fermentation medium are: chlorella powder 10g / l, glucose 20g / l, the rest is distilled water, sterilized at 115°C for 30min.

[0056] (3) Adjust pH: Use 1mol / L NaOH solution to adjust the pH of the fermentation broth ...

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Abstract

The invention discloses a chlorella fermentation extract and a preparation method thereof. According to the method, a culture medium of strains such as Lactobacillus plantarum is used to carry out liquid fermentation culture of a chlorella powder to obtain a fermentation product, and the fermentation product is subjected to centrifugation, filtration, and sterilization to obtain the chlorella powder fermentation extract. The method has mild reaction conditions, simple preparation process, and no any added chemical components. The experiments prove that the chlorella powder fermentation extractprepared by the method is rich in protein, crude polysaccharides, total amino acids, xanthophyll and other functional ingredients, has the effects such as free radical resistance, skin irritation improvement, and anti-aging, has high biological safety, and can be widely used in various cosmetics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a chlorella fermented extract and a preparation method thereof. Background technique [0002] Chlorella vulgaris (Chlorella vulgaris) Chlorophyceae, Chlorellaceae. Unicellular algae, often solitary, but also multicellular aggregates. One of the earliest life on earth is a highly efficient photosynthetic plant, which grows and reproduces by photoautotrophy, and is widely distributed. Due to its high application value, Chlorella, together with the development and utilization of microalgae such as Spirulina, Scenedesmus, and Salina, constitutes microalgae biotechnology, which has broad development prospects. [0003] Chlorella is rich in protein, with a content as high as 50-60%. It can be used as an important source of single-cell protein. It also contains various amino acids, dietary fiber, vitamins and other nutrients necessary for humans and animals. Chlorella also ha...

Claims

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Application Information

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IPC IPC(8): A61K8/9722A61Q19/08A61Q19/00C12P1/04C12R1/25C12R1/245C12R1/225C12R1/01
CPCA61K8/9722A61Q19/08A61Q19/00C12P1/04A61K2800/85C12R2001/245C12N1/205C12R2001/225C12R2001/25C12R2001/46
Inventor 马双双李海军苏移山李珍爱张英华王兆兰王庆波刘芳李松
Owner SHANDONG FREDA BIOTECH
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