Fruit fly trapping agent containing grape terpenoids, and application thereof
A technology of terpenoids and traps, applied in the field of agricultural science, can solve the problems of poor practicality, limited trapping effect, poor effect, etc., and achieve the effect of obvious control effect and reduction of pesticide usage.
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Embodiment 1
[0041] (1) Weigh 85g of corn flour, 10g of agar, 70g of sucrose, 18g of yeast powder, 5mL of propionic acid, and 1000mL of distilled water;
[0042](2) Boil 500mL of water, dissolve agar in a small amount of water and add it into the boiling water. After boiling, stir the sucrose, corn flour and 500mL of distilled water into the pot, and keep stirring until it becomes thick. Add yeast powder and stir evenly , after cease-fire, add propionic acid and stir thoroughly to obtain the basal medium required for the experiment;
[0043] (3) Dissolving linalool, geraniol, citral or geranic acid in distilled water as an attractant (both purchased from McLean Biochemical Technology Co., Ltd., with a content greater than 90%) to prepare a 125 μL / L aqueous attractant solution ;
[0044] (4) Take 5mL of the basal medium prepared in step (2) and put it into a clean and sterilized Petri dish. When the temperature of the basal medium drops to about 60°C, add 1mL of different kinds of attracta...
Embodiment 2
[0046] Prepare different kinds of attractant aqueous solutions with a concentration of 250 μL / L (the kind of attractant is the same as in Example 1), and add 1 mL of each to the prepared 5 mL of basal medium.
Embodiment 3
[0048] Prepare different kinds of attractant aqueous solutions with a concentration of 500 μL / L (the kind of attractant is the same as in Example 1), and add 1 mL of each to the prepared 5 mL of basal medium.
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