Glycosylated protein-polyphenol Pickering emulsion and preparation method thereof
The technology of glycosylated protein and polyphenol is applied in the field of glycosylated protein-polyphenol Pickering emulsion and its preparation, and can solve the problems of large particle size difference of Pickering emulsion, low storage stability, small protein particle and the like, Achieve the effect of improving storage stability, improving thermal stability and good uniformity
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[0030] This embodiment provides a preparation method of glycosylated protein-polyphenol Pickering emulsion, the preparation method comprising:
[0031] 1) Preparation of glycosylation reaction product
[0032] Mix and dissolve whey protein isolate and D-(+) lactose with a mass ratio of 1:1 in water to obtain a mixed solution, the total content of protein and D-(+) lactose in the mixed solution is 10wt%, and adjust the mixed solution pH to 6.5-7.5; the mixed solution was freeze-dried to obtain whey protein isolate-lactose mixed powder; the whey protein isolate-lactose mixed powder was subjected to Maillard reaction, and the condition of Maillard reaction was: the air humidity was 75 %, the temperature is 68°C, and the reaction time is 18h to obtain glycosylated whey protein isolate powder;
[0033] 2) Preparation of glycosylated whey protein isolate-polyphenol complex
[0034] Mix glycosylated whey protein isolate powder with water to obtain 5 mg / mL glycosylated whey protein ...
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