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Glycosylated protein-polyphenol Pickering emulsion and preparation method thereof

The technology of glycosylated protein and polyphenol is applied in the field of glycosylated protein-polyphenol Pickering emulsion and its preparation, and can solve the problems of large particle size difference of Pickering emulsion, low storage stability, small protein particle and the like, Achieve the effect of improving storage stability, improving thermal stability and good uniformity

Pending Publication Date: 2020-02-21
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the formed protein particles are small, and the stable Pickering emulsion has a large difference in particle size, and the storage stability is low, which cannot meet the needs of shelf life.

Method used

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  • Glycosylated protein-polyphenol Pickering emulsion and preparation method thereof
  • Glycosylated protein-polyphenol Pickering emulsion and preparation method thereof
  • Glycosylated protein-polyphenol Pickering emulsion and preparation method thereof

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Embodiment 1

[0030] This embodiment provides a preparation method of glycosylated protein-polyphenol Pickering emulsion, the preparation method comprising:

[0031] 1) Preparation of glycosylation reaction product

[0032] Mix and dissolve whey protein isolate and D-(+) lactose with a mass ratio of 1:1 in water to obtain a mixed solution, the total content of protein and D-(+) lactose in the mixed solution is 10wt%, and adjust the mixed solution pH to 6.5-7.5; the mixed solution was freeze-dried to obtain whey protein isolate-lactose mixed powder; the whey protein isolate-lactose mixed powder was subjected to Maillard reaction, and the condition of Maillard reaction was: the air humidity was 75 %, the temperature is 68°C, and the reaction time is 18h to obtain glycosylated whey protein isolate powder;

[0033] 2) Preparation of glycosylated whey protein isolate-polyphenol complex

[0034] Mix glycosylated whey protein isolate powder with water to obtain 5 mg / mL glycosylated whey protein ...

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Abstract

The invention discloses a glycosylated protein-polyphenol Pickering emulsion and a preparation method thereof. The preparation method comprises the following steps: 1) preparation of a glycosylation reaction product; 2) preparation of a glycosylated protein-polyphenol complex; and 3) preparation of the glycosylated protein-polyphenol Pickering emulsion. The thermal stability of oil-water interfaceprotein is improved by Maillard reaction, and the stability of the Pickering emulsion is mainly provided by an interface particle layer, so that the purpose of improving acid heat stability of the emulsion is achieved; and meanwhile, epigallocatechin gallate is selected to effectively regulate the particle size of a glycosylated whey protein isolate, the prepared glycosylated protein-polyphenol complex with proper particle size can effectively inhibit the coalescence phenomenon among oil drops, emulsion drops show good homogeneity, the homogeneity of the emulsion drops is directly related tostorage stability, so that the purpose of improving the storage stability of the emulsion is achieved.

Description

technical field [0001] The invention belongs to the field of protein-based emulsion carriers, and more specifically relates to a glycosylated protein-polyphenol Pickering emulsion and a preparation method thereof. Background technique [0002] Pickering emulsions have received considerable attention in the pharmaceutical, cosmetic, and food industries due to their high stability, low toxicity, and controllable particle size. Pickering emulsions are stabilized by adsorbing particles at the oil-water interface and form a thin shell structure to prevent coalescence. The particles that stabilize Pickering emulsions are mainly derived from natural and synthetic materials. However, synthesizing materials involves toxic chemical agents and is restricted in the food and pharmaceutical industries. Therefore, natural, environmentally friendly edible material protein can be used as a good pickering stabilizer. Whey protein isolate (WPI) is a by-product of cheese manufacturing and ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L29/00C08J3/09C07K1/107C08L89/00
CPCA23L29/03A23L29/045A23L29/035C08J3/095C07K1/1077C08J2389/00
Inventor 刘刚王麒胡中泽秦新光王学东李宛蓉
Owner WUHAN POLYTECHNIC UNIVERSITY