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Application of resveratrol and combination of resveratrol and ketoconazole in preparation of medicaments for resisting fungal infection diseases

A technology combining ketoconazole and resveratrol is applied in the direction of antifungal agents, medical preparations containing active ingredients, active ingredients of hydroxyl compounds, etc. , the effect of fast reproduction

Active Publication Date: 2020-02-21
陕西科聚美康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The object of the present invention is to: overcome the problems of current fungal drug resistance and large toxic and side effects of antifungal drugs, and provide an antifungal pharmaceutical composition, which uses resveratrol and ketoconazole as main active ingredients

Method used

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  • Application of resveratrol and combination of resveratrol and ketoconazole in preparation of medicaments for resisting fungal infection diseases
  • Application of resveratrol and combination of resveratrol and ketoconazole in preparation of medicaments for resisting fungal infection diseases
  • Application of resveratrol and combination of resveratrol and ketoconazole in preparation of medicaments for resisting fungal infection diseases

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Effect test

Embodiment 1

[0031] Example 1: Application of resveratrol combined with ketoconazole in the preparation of antifungal products

[0032] The present invention provides an application of resveratrol combined with ketoconazole in preparing antifungal products.

[0033] Preferably, the fungus is Trichophyton rubrum or Candida albicans or Microsporum gypsum.

[0034] The resveratrol is preferably obtained by fermentation production and metabolism by Myxococcus virescens.

[0035] The present invention provides an application of resveratrol combined with ketoconazole in the preparation of antifungal products. When it is used as an anti-Candida albicans product, the usage amount of resveratrol is 50-400μg / mL, and ketoconazole The usage amount is 0.09-22.5μg / mL.

[0036] Preferably, when it is used as an anti-Candida albicans product, the optimal usage amount of resveratrol is 200 μg / mL, and the usage amount of ketoconazole is 11.25 μg / mL.

[0037] The present invention provides an application of resveratro...

Embodiment 2

[0041] Example 2: Preparation of resveratrol

[0042] The experimental strain is the purchased Myxococcus virescens, which was cultured in the fermentation medium HT medium, filtered through the fermentation broth, adsorbed with HP20 macroporous resin, and washed with 10 times the resin volume of water and methanol in sequence. Take off. The methanol eluent was evaporated under reduced pressure at 50°C to remove the solvent to obtain a crude methanol extract. The crude methanol extract was subjected to silica gel column chromatography, CHCl 3 -MeOH gradient elution, each 100mL eluent is a collection unit, TLC detects the elution progress. Use ketoconazole and each component to conduct interactive activity tracking screening of Candida albicans, select several parts with better activity for separation and purification, and finally collect the active monomer compounds with the preparation liquid phase. The structure of the compound was identified by 1H-NMR. Appraisal results are...

Embodiment 3

[0046] Example 3: The half inhibitory concentration of resveratrol against Candida albicans (MIC 50 ) Detection

[0047] Based on Example 2, the compound 1 trans-resveratrol prepared above was dissolved in 100% dimethyl sulfoxide (DMSO) to prepare a resveratrol-like liquid with a concentration of 20 mg / ml. In the dilution method, the resveratrol sample solution was added to the Sabouraud broth medium until the resveratrol concentration was 8000, 4000, 2000, 1000, 500, 250, 125 μg / mL, respectively. Take a 96-well bacterial culture plate and add 100μL to each well at a concentration of 10 5 CFU / ml Candida albicans bacterial solution and 100μL resveratrol sample solution of various concentrations, and set a blank control (medium without resveratrol sample solution + bacterial solution) and solvent control (DMSO solution + bacterial solution ), and set up a control culture plate (without adding bacteria). Incubate in a 30 degree incubator for 24 hours, take out the 96-well culture p...

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Abstract

The invention discloses an application of resveratrol and combination of resveratrol and ketoconazole in preparation of medicaments for resisting fungal infection diseases. According to the invention,resveratrol and ketoconazole medicaments are combined for the first time and a synergistic antifungal effect is achieved, so that the using concentration of ketoconazole can be obviously reduced, thebacteriostatic effects on candida albicans, trichophyton rubrum and microsporum gypsum are all higher than effects of single use of resveratrol or ketoconazole, the bacteriostatic rates all reach 90%or above, and the bacteriostatic rates are obviously improved. Resveratrol and ketoconazole are combined in preparation of medicaments for resisting fungal infection diseases, and the application hasa wide development and application prospect.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to the technical field of application of resveratrol combined with ketoconazole in the preparation of antifungal products. Background technique [0002] Fungi are a type of eukaryotic cell organisms that exist in nature. They are diverse and widely distributed, and can infect different parts of humans and animals. Fungal infections can be divided into two types: superficial fungal infections and deep fungal infections: superficial fungal infections are usually caused by skin fungal infections, infecting the epidermis, hands, feet, hair, nails and other parts, usually mainly manifested as ringworm, dermatitis, etc. It is difficult to cure and easy to recur; deep fungal infections include subcutaneous tissue infections and systemic fungal infections. Systemic fungal infections can infect human subcutaneous tissues, internal organs, blood, bones and central nervous system, and can cause de...

Claims

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Application Information

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IPC IPC(8): A61K31/496A61K31/05A61P31/10
CPCA61K31/496A61K31/05A61P31/10A61K2300/00
Inventor 万一王军张绪高磊张月娟王璐
Owner 陕西科聚美康生物科技有限公司
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