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A Chlorella exopolysaccharide with antioxidant activity

A technology of antioxidant activity and extracellular polysaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, fuels, etc., can solve the difficulty of separation and extraction, increase the cost of separation and extraction, and produce little effect on the cost of microalgae oil production, etc. problems, to achieve significant social and economic benefits and reduce production costs

Active Publication Date: 2022-03-01
XIANGTAN UNIV
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Problems solved by technology

Although the above-mentioned strategy of using microalgae oil to co-produce other intracellular components simultaneously expands the application range of microalgae and reduces the production cost of microalgae oil to a certain extent, the above-mentioned co-production components are all intracellular components. It greatly increases the cost of separation and extraction in the subsequent separation and preparation process, and some components such as astaxanthin exist in algae cells in the form of esterification, which is difficult to separate and extract, and has little effect on reducing the production cost of microalgae oil

Method used

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  • A Chlorella exopolysaccharide with antioxidant activity
  • A Chlorella exopolysaccharide with antioxidant activity
  • A Chlorella exopolysaccharide with antioxidant activity

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Embodiment 1

[0027] 1) Liquid culture of Chlorella under salt stress in conjunction with light-dark alternating mode

[0028] In a conventional aseptic operation method, Chlorella protothecoides CS-41 was inoculated on Basal solid medium, and cultivated under light for 5 days to obtain rejuvenated Chlorella protothecoides. A single colony of the rejuvenated Chlorella species was picked and inoculated in liquid Basal medium for 5 days of light culture to obtain a first-grade Chlorella seed liquid. The primary Chlorella seed solution was inoculated into the liquid Basal medium with 5% inoculation amount, and cultured in the dark for 5 days to obtain the secondary seed solution. Then inoculate 1% secondary seed solution in Basal liquid medium containing sodium chloride (10‰ salinity), and cultivate it for 7 days under light-dark alternation (8h light: 16h dark) mode to obtain Chlorella culture liquid.

[0029] 2) Separation, purification and preparation of chlorella exopolysaccharide

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Abstract

The invention discloses a chlorella exopolysaccharide with antioxidant activity. The exopolysaccharide of chlorella of the present invention is obtained by the secretion and separation and purification of chlorella in liquid culture of salt stress in conjunction with light-dark alternating mode. The compositions were 19.81%, 79.93% and 0.25% xylose, mannose and ribose, respectively. The present invention is the first time to isolate the polysaccharide secreted from the outside of the cell from the chlorella fermentation medium environment, and the obtained chlorella exopolysaccharide has obvious scavenging effects on DPPH free radicals, superoxide anions, hydroxyl free radicals, and ABTS free radicals. dose effect. The use of exopolysaccharides secreted to the medium during microalgae cultivation can not only fully tap the high added value of these by-products, but also provide new ideas for reducing the production cost of microalgae oils for biodiesel production. Its social and economic benefits significantly.

Description

technical field [0001] The invention relates to the field of development and application of food resources, in particular to a chlorella exopolysaccharide with antioxidant activity. Background technique [0002] Microalgae have become one of the ideal raw materials for the preparation of biofuels due to their advantages such as short growth cycle, no occupation of agricultural land, and high oil synthesis efficiency. Their research and development investment has positive significance for alleviating my country's energy pressure, controlling air pollution, and sustainable social and economic development. . However, microalgae-based biofuel refining has not been industrialized due to its low economic feasibility, and one of the main reasons is the high production cost of microalgae oil. Therefore, how to reduce the cost of microalgae oil is one of the effective ways to realize the commercialization of microalgae biodiesel. [0003] At present, many researchers have expanded m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C10L1/02C12P19/04C12R1/89
CPCC08B37/006C10L1/02C12P19/04Y02E50/10
Inventor 李玉芹雷铮宇周蓉唐裕芳沈晓静贾淑婷
Owner XIANGTAN UNIV
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