Method for quickly separating RBCs used for immunochromatography kit

An immunochromatographic detection and red blood cell technology, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of flow influence of chromatographic reaction, background interference, influence of immunochromatographic reaction, etc., to ensure biological activity and good biological phase. The effect of capacitive and good binding ability

Active Publication Date: 2020-02-21
GUANGZHOU KEFEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in these methods, on the one hand, red blood cells still exist in the reaction system on the immunological reagent strip, which has a significant impact on the flow of the chromatographic reaction
On the other hand, when there is hemolysis in the whole blood sample to b...

Method used

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  • Method for quickly separating RBCs used for immunochromatography kit
  • Method for quickly separating RBCs used for immunochromatography kit
  • Method for quickly separating RBCs used for immunochromatography kit

Examples

Experimental program
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Embodiment 1

[0034] The characteristics of the separation of red blood cells in this implementation include: carboxyl polystyrene microspheres at 3.0%, polylysine at 0.05%, and anti-human RBC antibodies at 0.05 mg / mL. The preparation method is as follows:

[0035] S1. Preparation of polystyrene microspheres modified with polylysine

[0036] ① Take 1.0 mL of 3.0% carboxyl polystyrene microspheres, add it to 9.0 mL of microsphere diluent, and ultrasonically disperse for 10 minutes to obtain a uniformly dispersed microsphere suspension;

[0037] ② Under rapid stirring, add 10-fold concentration of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) / N to the above uniformly dispersed microsphere suspension -Hydroxysuccinimide (NHS) activator to activate carboxyl polystyrene microspheres for 10-120min. In the activation reaction system, the reaction concentration used by EDC is 100 mM, and the reaction concentration used by NHS is 100 mM.

[0038] ③After the activation, the reaction sol...

Embodiment 2

[0047] The only difference between this embodiment and Embodiment 1 is that the concentration of polylysine used in this embodiment is 0.1%.

Embodiment 3

[0049] The only difference between this example and Example 1 is that the active group dendrimer used in this example is dendrimer polyamidoamine, and its concentration is 0.1%.

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PUM

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Abstract

The invention provides a method for separating RBCs (Red Blood Cell) from whole blood, wherein the core of the method is an RBC separating agent which comprises the following components: carboxyl polystyrene microspheres, active group dendritic polymers and anti-human RBC antibodies; wherein the active group dendritic polymer is polylysine, dendritic polyamidoamine or polypropylenimine n-dotriacontane amine. The RBC separating agent is realized by utilizing a polylysine modified polystyrene microsphere-antihuman RBC antibody compound, the RBC separating agent is added into a sample diluent ofan immunochromatography kit or a specific blood processing tube thereof in advance, the RBC separating agent can be quickly combined with RBCs in a test sample in the testing process to quickly form ahuge reticular structure, and quick sedimentation is realized under the action of gravity, so that a supernatant required by reaction is obtained.

Description

Technical field [0001] The present invention relates to the technical field of biological preparations, in particular to a method for rapidly separating and removing red blood cells from whole blood. Background technique [0002] Point of care testing (POCT) refers to rapid testing that can be performed at the patient's medical field. Its core is to meet the rapid diagnosis requirements required for clinical treatment or home monitoring, and provide accurate and reliable test results in a timely manner. With its advantages of simple operation and rapid response, POCT has gradually become an important means for clinical doctors to judge the condition. [0003] With the continuous development of clinical testing technology, in order to meet the needs of rapid bedside testing, testing reagents cover more and more types of blood samples, not only for serum and plasma samples, but also for whole blood samples. To achieve rapid detection. However, the red blood cells in the whole blood...

Claims

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Application Information

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IPC IPC(8): G01N33/531C08J7/16C08L25/06
CPCG01N33/531C08J7/16C08J2325/06
Inventor 孙子洪
Owner GUANGZHOU KEFEN BIOTECH CO LTD
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