Theaflavin muscle-strengthening dietary nutritional supplement and preparation method thereof
A technology of nutritional supplements and theaflavins, which is applied in the field of dietary nutritional supplements of fermented tea extract theaflavins TFs and its preparation, can solve the problems of no specific disclosure, etc., so as to delay muscle-related diseases, delay aging, enhance The effect of muscle strength
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[0031] (1) Preparation of polyphenol oxidase: Take 100g of fresh tea leaves, add NaHCO3 buffer solution with double volume, 3~5°C, pH7.5, mix well, then beat, add NaHCO3 with double volume, pH7.5 Buffer solution, mixed evenly, then gauze filtration to obtain the filtrate, high-speed centrifugation at a speed of 4000r / min at a temperature of 4 to 5°C to obtain a supernatant that is polyphenol oxidase, which is stored in a freezer at -20°C for subsequent use;
[0032](2) Fermentation: Weigh 1000g of catechin components in a certain proportion, including epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (epicatechin, , ECG) and epigallocatechin gallate (epigallocatechin gallate, EGCG), the selected catechin monomers accounted for 18%, 8%, 22%, and 52% by weight, respectively. After fully dissolving 10 times the weight of deionized water, adjust the pH to 5.0~6.0 with 10% citric acid, add the polyphenol oxidase obtained in step (1), and ferment with oxygen at 20~24°C f...
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