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Ultrasound-assisted extraction method for xanthine oxidase inhibitors in perilla leaves

A xanthine oxidase, ultrasonic-assisted extraction technology, applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc. High, easy-to-use effects

Pending Publication Date: 2020-02-28
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports on the extraction of xanthine oxidase inhibitors from perilla leaves at home and abroad, but there are no in-depth and detailed studies and reports on the process of extracting xanthine oxidase inhibitors from perilla leaves.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Dry the harvested perilla leaves naturally, remove impurities, crush them, pass through a 60-mesh sieve, and store in a sealed bag for later use; accurately weigh 1.00g of perilla leaf powder, put it into a 50mL conical flask, and press 1:30 Ratio of solid to liquid Add 50% ethanol solution, mix thoroughly, ultrasonically assist extraction for 20min under the conditions of extraction temperature of 40°C and ultrasonic power of 165W, centrifuge the extract at 5000r / min for 15min, collect the supernatant, and concentrate under reduced pressure , freeze-drying, to obtain the crude product of the xanthine oxidase inhibitor in perilla leaves, which is detected by ultraviolet spectrophotometry, and its inhibition rate to xanthine oxidase is 68.99%. Dissolve the crude product of xanthine oxidase inhibitor in deionized water, prepare a 15mg / mL sample solution, weigh 7g of NKA-9 macroporous resin for wet packing, and carry out the sample solution at a flow rate of 3BV / h Load the...

Embodiment 2

[0026] Dry the harvested perilla leaves naturally, remove impurities, crush them, pass through a 60-mesh sieve, and store them in sealed bags for later use. Accurately weigh 1.00g perilla leaf powder, put it into a 50mL Erlenmeyer flask, add 40% ethanol solution according to the ratio of solid to liquid of 1:30, mix well, and ultrasonically assist at the extraction temperature of 40°C and ultrasonic power of 165W Extract for 10 minutes, centrifuge the extract at 5000r / min for 15 minutes, collect the supernatant, concentrate under reduced pressure, and freeze-dry to obtain the crude product of xanthine oxidase inhibitor in perilla leaves. The inhibition rate was 66.76%. Dissolve the crude product of xanthine oxidase inhibitor in deionized water, prepare a 15 mg / mL sample solution, weigh 7 g of NKA-9 macroporous resin, and carry out wet packing, and load the sample at a flow rate of 3 BV / h. Wash with 5 column volumes of deionized water to remove impurities, elute with 70% ethan...

Embodiment 3

[0028] Harvest the perilla leaves, dry them naturally, remove impurities, crush them through a 60-mesh sieve, and store them in sealed bags for later use. Accurately weigh 1.00g perilla leaf powder, put it into a 50mL Erlenmeyer flask, add 50% ethanol solution at a ratio of 1:30 to liquid, mix thoroughly, and extract with ultrasonic assistance at an extraction temperature of 30°C and an ultrasonic power of 165W 30min, the extract was centrifuged at 5000r / min for 15min, the supernatant was collected, concentrated under reduced pressure, and freeze-dried to obtain the crude product of xanthine oxidase inhibitor in perilla leaves, which was detected by ultraviolet spectrophotometry, and its inhibitory effect on xanthine oxidase The inhibition rate was 50.84%. Dissolve the crude product of xanthine oxidase inhibitor in deionized water, prepare a 15 mg / mL sample solution, weigh 7 g of NKA-9 macroporous resin, and carry out wet packing, and load the sample at a flow rate of 3 BV / h. ...

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PUM

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Abstract

The invention discloses an ultrasound-assisted extraction method for xanthine oxidase inhibitors in perilla leaves. The ultrasound-assisted extraction method includes the following steps: subjecting the harvested perilla leaves to natural drying, impurity removal and crushing, dissolving the crushed leaves in an ethanol solution, performing ultrasonic extraction at the temperature of 30-70 DEG C for 10-50 minutes, removing residues through centrifugal filtration, collecting supernatant, concentrating the supernatant under reduced pressure, performing freeze-drying, dissolving crude products ofthe xanthine oxidase inhibitors in deionized water to prepare 5-20 mg / mL of loading solutions, performing loading at the flow rate of 2-5B V / h, removing impurities by 5 column volumes of deionized water, eluting with 40-70% of ethanol at the flow rate of 1-4 BV / h, collecting eluent, concentrating the eluent under reduced pressure, and performing freeze-drying to obtain the xanthine oxidase inhibitors. The ultrasound-assisted extraction method is simple to operate, high in efficiency and low in cost, the obtained xanthine oxidase inhibitors have the advantages of high purity, high inhibitory activity and the like, and a certain theoretical and technical support is provided for treatment of gout or hyperuricemia.

Description

technical field [0001] The invention relates to the field of natural plant extraction, in particular to a method for ultrasonically assisted extraction of xanthine oxidase inhibitors from perilla leaves. Background technique [0002] Hyperuricemia and gout are common and frequently-occurring diseases in today's society, and there is no cure so far. In recent years, the incidence of gout has continued to increase, and it is gradually becoming younger. Hyperuricemia is a purine metabolic disease caused by genetic or acquired factors. It is caused by increased production or decreased excretion of uric acid in the human body. It has been identified as a risk factor for cardiovascular diseases. High concentrations of serum uric acid can directly damage the kidneys, leading to renal fibrosis, renal failure, and hypertension. When uric acid in patients with hyperuricemia is deposited in the joints and soft tissues in the form of sodium salt, it will cause tissue inflammation and ...

Claims

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Application Information

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IPC IPC(8): A61K36/535A61P19/06A61K127/00
CPCA61K36/535A61K2236/15A61K2236/17A61K2236/333A61K2236/51A61K2236/55A61P19/06
Inventor 李会珍张志军王亚楠崔丽霞陈铁
Owner ZHONGBEI UNIV