Calprotectin detection kit and detection method

A technology for detecting kits and calprotectin, which is applied in the field of medical testing, can solve the problems of low accuracy of test results, high production cost of calprotectin detection kits, and low sensitivity, so as to avoid colonoscopy invasive examination and improve The effect of detection sensitivity and wide detection range

Pending Publication Date: 2020-02-28
JIANGSU INNOVATION BIOTECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] Related calprotectin detection kits are disclosed in the prior art, such as a human serum calprotectin detection kit and its preparation method with the patent number CN110161254A, and a fast and efficient

Method used

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  • Calprotectin detection kit and detection method
  • Calprotectin detection kit and detection method
  • Calprotectin detection kit and detection method

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Experimental program
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Embodiment

[0052] Calprotectin detection kit of the present invention, its composition is as shown in the following table:

[0053]

[0054]

[0055] This kit adopts the double-antibody sandwich immunoassay method. The microwell plate contains immobilized specific antibodies. Samples and enzyme-labeled antibodies are added to the reaction wells of the enzyme-labeled plate. The solid-phase immune complex is washed to remove unbound enzyme-labeled antibodies. At this time, the amount of enzyme on the solid-phase carrier is directly related to the content of the antigen to be tested in the sample. After adding a luminescent substrate, measure the luminous intensity (RLU). The RLU value of the sample increases as the concentration increases, and the sample content can be calculated through the standard curve.

[0056] Among the present invention, the configuration of various solution liquids is as follows:

[0057] Preparation of coating buffer:

[0058] Standard formula:

[0059] M...

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Abstract

The invention relates to a calprotectin detection kit and a detection method. The kit mainly comprises a microwell plate coated with calprotectin antibodies, a calprotectin calibrator, calprotectin antibodies labeled by horseradish peroxidase, an extraction buffer solution, a sample diluent, a substrate solution A (luminol solution), a substrate solution B (hydrogen peroxide solution) and a concentrated washing solution. According to the invention, a double-antibody sandwich immunodetection method is adopted; the microporous plate comprises an immobilized specific antibody; a sample and an enzyme-labeled antibody are added into a reaction hole of an enzyme-labeled plate, and a solid-phase immune complex containing a specific antibody-antigen-enzyme-labeled antibody is formed after a heat preservation reaction; the uncombined enzyme-labeled antibody is removed through washing, and the kit is suitable for large-batch detection; the kit is easy to operate, high in sensitivity, good in specificity, rapid and effective in detection and low in cost and has the application and popularization value.

Description

Technical field: [0001] The invention relates to the technical field of medical testing, in particular to a calprotectin detection kit. Background technique: [0002] Inflammatory bowel disease (IBD) is a group of chronic nonspecific inflammatory diseases of the intestinal tract with unclear etiology, including ulcerative colitis (UC) and Crohn's disease (CD). It is known that the inflammatory response caused by abnormal intestinal mucosal immune system plays an important role in the pathogenesis of IBD. Currently, the diagnosis of IBD mainly relies on medical history, clinical symptoms, colonoscopy, and pathological tissue biopsy. Although pathological tissue biopsy is the gold standard for diagnosis, it often delays diagnosis and treatment because of its pain, high cost, and inconvenient repeated examination. [0003] Calprotectin is a protein that binds calcium and zinc with a relative molecular mass of 36×10 3 , derived from neutrophils and activated macrophages, has ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/58
CPCG01N33/6893G01N33/577G01N33/581G01N2800/065G01N2800/067
Inventor 雷瑞斌刘琴刘扬吴红兵李慧成
Owner JIANGSU INNOVATION BIOTECH CO LTD
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