A method for constructing a three-dimensional vascular network hydrogel

Abstract

The method for constructing the three-dimensional vascular network hydrogel provided by the embodiment of the present invention firstly pours the vascular filler into the blood vessels of the isolated organ, and after the vascular filler hardens, peels off the isolated organ tissue to obtain the isolated organ tissue. The three-dimensional blood vessel model of the organ, and then put the three-dimensional blood vessel model into the mold, pour the hydrogel prepolymer into the mold after low-temperature polymerization, and obtain the hydrogel model, and extract the three-dimensional blood vessel in the hydrogel model The three-dimensional vascular network is obtained by the method, which is simple in technology, does not require complex processing, and has low requirements for equipment. The structure of the three-dimensional blood vessel model constructed is the same as that of the biological microvascular network, and has the characteristics of complete bionics, and the constructed three-dimensional blood vessels The model has high tensile strength, good elasticity, is easy to pull out, does not remain in the gel, has good biocompatibility, and solves the problems existing in the construction of a three-dimensional vascular network in the prior art.

Description

technical field [0001] The invention belongs to the technical field of tissue biomanufacturing, and in particular relates to a method for constructing a three-dimensional blood vessel network hydrogel. Background technique [0002] With the development of tissue engineering, research related to tissue engineering, such as the manufacture of skin, ear, and cartilage, etc., has developed significantly in recent years. However, there are still many problems in the construction of large-volume tissues, among which vascularization is one of the main difficulties currently faced. [0003] In the constructed engineered tissue, cells must be close enough to the vascular network (100–200 μm) to obtain oxygen and nutrient supply, thereby preventing the formation of necrotic cores. However, when the engineered tissue is implanted into the host, host capillary sprouting and invasion into the engineered scaffold are slow, so the most important issue in the tissue fabrication process is ...

AI Technical Summary

Problems solved by technology

However, the microchannels formed by these methods are usually limited to a two-dimensional plane, and rely on multiple layer-by-layer assembly steps, the preparation process is complicated, and it is easy to cause poor alignment of the interface in engineered tissues.

It is also possible to use bioprinting technology to manufacture microchannels, for example, print a designed three-dimensional vascular network model with Pluronic F127, sodium alginate and agarose, and then immerse the template in the hydrogel prepolymer, and wait for the hydrogel to After molding, the sacrificial template is removed to form a scaffold with a three-dimensional vascular network. However, the use of bioprinting technology not only requires high printer parameters but also high requirements for printing materials, and the three-dimensional structure of the manufactured vascular network is large in size, which is not suitable for When the small-scale three-dimensional vascular network of the human body has multiple branches, it is not easy to pull out, and it is easy to remain in the glue

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