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Application of qi-tonifying and pulse-restoring (freeze-drying) or ruscogenin for injection in prevention of pharmaceutical organ hemorrhage

A technology of ruscogenin, nourishing qi and Fumai, which can be applied in the directions of drug delivery, drug combination, and drug formulation, etc., can solve the problems of increasing endothelial cell permeability, vascular leakage, etc.

Inactive Publication Date: 2020-03-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dasatinib is usually associated with hemorrhagic complications. It phosphorylates MLC through the ROCK pathway, induces the polymerization of F-actin structure, and remodels the cytoskeleton; Dasatinib induces changes in the structure of VE-cadherin between endothelial cells , increase the permeability of endothelial cells, resulting in blood vessel leakage, so side effects such as gastrointestinal bleeding occur

Method used

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  • Application of qi-tonifying and pulse-restoring (freeze-drying) or ruscogenin for injection in prevention of pharmaceutical organ hemorrhage
  • Application of qi-tonifying and pulse-restoring (freeze-drying) or ruscogenin for injection in prevention of pharmaceutical organ hemorrhage
  • Application of qi-tonifying and pulse-restoring (freeze-drying) or ruscogenin for injection in prevention of pharmaceutical organ hemorrhage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Improvement effect of Yiqi Fumai for injection (lyophilized) and its active ingredient (ruscogenin) on dasatinib-induced gastrointestinal bleeding in mice.

[0039] experimental method:

[0040] a) Experimental animals

[0041] The experimental animals are C57BL / 6J mice, weighing 18-22g, SPF grade, purchased from the Comparative Medicine Center of Yangzhou University, which meet the quality standards of ordinary experimental animals. License number: SCXK (Su) 2012-0001. The mice were reared in separate cages, free to drink and eat, the room temperature was 23-25°C, the relative humidity was 40%-80%, and the experiment was started after about one week of adaptive feeding.

[0042] b) Preparation of experimental drugs

[0043] Weigh 33.6mg of YQFM, add 1mL of normal saline solution, fully dissolve, this is the drug concentration of the low dose group; weigh 67.1mg of YQFM, add 1mL of normal saline solution, fully dissolve, this is the drug concentration of the middle d...

Embodiment 2

[0050] Yiqi Fumai injection (freeze-dried) and its active ingredient ruscogenin in improving the barrier damage of human umbilical vein endothelial cells induced by dasatinib.

[0051] experimental method:

[0052] a) Cell culture: HUVECs were cultured with 10% FBS RPMI 1640 medium after recovery. When the cells are about 90% confluent, discard the medium and rinse with PBS. Add 0.25% trypsin solution, digest in a 37°C incubator, observe the roundness of the cells under a microscope, discard the digestion solution and add RPMI 1640 culture solution containing 10% FBS to terminate the digestion. Blow down the cells and transfer to a centrifuge tube, and collect the cells by centrifugation. Discard the supernatant, add RPMI 1640 culture medium containing 10% FBS and blow it into a cell suspension, pass on a new cell culture dish, and culture at 37°C in a 5% CO2 incubator.

[0053] b) Measurement of transendothelial cell resistance: 200 μl (3-5x10 4 pcs / well) into the Millice...

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Abstract

The invention discloses a new application of qi-tonifying and pulse-restoring (freeze-drying) or ruscogenin for injection in prevention of pharmaceutical organ hemorrhage. A dasatinib-induced mouse gastrointestinal hemorrhage model is used for verifying the improvement effect of qi-tonifying and pulse-restoring (freeze-drying) or ruscogenin for injection on dasatinib-induced mouse gastrointestinalhemorrhage. In addition, a dasatinib damaged HUVEC cell model is also used for verifying the improvement effect of the qi-tonifying and pulse-restoring (freeze-drying) or luscogenin for injection onthe cell permeability.

Description

technical field [0001] The invention relates to the fields of traditional Chinese medicine compound prescriptions and natural medicines, in particular to the injection of Yiqifumai (freeze-dried) or ruscogenin for preventing side effects of drug sexual organ bleeding. Background technique [0002] Ruscogenin (Ruscogenin, RUS) is the main saponin and one of the active ingredients in the traditional Chinese medicine Ophiopogon japonicus, also known as pseudophylloside. It has significant anti-inflammatory, anti-thrombotic, anti-tumor, and reduced capillary permeability effects ((1) KouJ, Sun Y, Lin Y, et al.Anti-inflammatory Activities of Aqueous Extract from Radix Ophiopogon japonicus and Its Two Constituents[ J].Biol Pharm Bull.2005,28(7):1234-1238.(2)Cheng ZH,Wu T,Yu BY.Steroidal glycosides from tubers ofOphiopogon japonicus[J].J Asian Nat Prod Res.2006,8( 6):555-559.(3)Kou J,TianY,Tang Y.Antithrombotic Activities of Aqueous Extract from RadixOphiopogonjaponicus and Its Tw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61P7/04
CPCA61K31/58A61K9/0019A61P7/04
Inventor 寇俊萍张媛媛刘元恺余伯阳徐晗
Owner CHINA PHARM UNIV
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